Scaffolds Loaded with Dialdehyde Chitosan and Collagen—Their Physico-Chemical Properties and Biological Assessment
In this work, dialdehyde chitosan (DAC) and collagen (Coll) scaffolds have been prepared and their physico-chemical properties have been evaluated. Their structural properties were studied by Fourier Transform Infrared Spectroscopy with Attenuated Internal Reflection (FTIR–ATR) accompanied by evalua...
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MDPI AG
2022-04-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/9/1818 |
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| author | Sylwia Grabska-Zielińska Judith M. Pin Beata Kaczmarek-Szczepańska Ewa Olewnik-Kruszkowska Alina Sionkowska Fernando J. Monteiro Kerstin Steinbrink Konrad Kleszczyński |
| author_facet | Sylwia Grabska-Zielińska Judith M. Pin Beata Kaczmarek-Szczepańska Ewa Olewnik-Kruszkowska Alina Sionkowska Fernando J. Monteiro Kerstin Steinbrink Konrad Kleszczyński |
| author_sort | Sylwia Grabska-Zielińska |
| collection | DOAJ |
| description | In this work, dialdehyde chitosan (DAC) and collagen (Coll) scaffolds have been prepared and their physico-chemical properties have been evaluated. Their structural properties were studied by Fourier Transform Infrared Spectroscopy with Attenuated Internal Reflection (FTIR–ATR) accompanied by evaluation of thermal stability, porosity, density, moisture content and microstructure by Scanning Electron Microscopy—SEM. Additionally, cutaneous assessment using human epidermal keratinocytes (NHEK), dermal fibroblasts (NHDF) and melanoma cells (A375 and G-361) was performed. Based on thermal studies, two regions in DTG curves could be distinguished in each type of scaffold, what can be assigned to the elimination of water and the polymeric structure degradation of the materials components. The type of scaffold had no major effect on the porosity of the materials, but the water content of the materials decreased with increasing dialdehyde chitosan content in subjected matrices. Briefly, a drop in proliferation was noticed for scaffolds containing 20DAC/80Coll compared to matrices with collagen alone. Furthermore, increased content of DAC (50DAC/50Coll) either significantly induced the proliferation rate or maintains its ratio compared to the control matrix. This delivery is a promising technique for additional explorations targeting therapies in regenerative dermatology. The using of dialdehyde chitosan as one of the main scaffolds components is the novelty in terms of bioengineering. |
| first_indexed | 2024-03-10T03:46:50Z |
| format | Article |
| id | doaj.art-5b9bedc331044b5aa97f656bfc334bbc |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T03:46:50Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-5b9bedc331044b5aa97f656bfc334bbc2023-11-23T09:06:44ZengMDPI AGPolymers2073-43602022-04-01149181810.3390/polym14091818Scaffolds Loaded with Dialdehyde Chitosan and Collagen—Their Physico-Chemical Properties and Biological AssessmentSylwia Grabska-Zielińska0Judith M. Pin1Beata Kaczmarek-Szczepańska2Ewa Olewnik-Kruszkowska3Alina Sionkowska4Fernando J. Monteiro5Kerstin Steinbrink6Konrad Kleszczyński7Department of Physical Chemistry and Physicochemistry of Polymers, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100 Toruń, PolandDepartment of Dermatology, University of Münster, Von-Esmarch-Str. 58, 48149 Münster, GermanyDepartment of Biomaterials and Cosmetics Chemistry, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100 Toruń, PolandDepartment of Physical Chemistry and Physicochemistry of Polymers, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100 Toruń, PolandDepartment of Biomaterials and Cosmetics Chemistry, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100 Toruń, Polandi3S—Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-180 Porto, PortugalDepartment of Dermatology, University of Münster, Von-Esmarch-Str. 58, 48149 Münster, GermanyDepartment of Dermatology, University of Münster, Von-Esmarch-Str. 58, 48149 Münster, GermanyIn this work, dialdehyde chitosan (DAC) and collagen (Coll) scaffolds have been prepared and their physico-chemical properties have been evaluated. Their structural properties were studied by Fourier Transform Infrared Spectroscopy with Attenuated Internal Reflection (FTIR–ATR) accompanied by evaluation of thermal stability, porosity, density, moisture content and microstructure by Scanning Electron Microscopy—SEM. Additionally, cutaneous assessment using human epidermal keratinocytes (NHEK), dermal fibroblasts (NHDF) and melanoma cells (A375 and G-361) was performed. Based on thermal studies, two regions in DTG curves could be distinguished in each type of scaffold, what can be assigned to the elimination of water and the polymeric structure degradation of the materials components. The type of scaffold had no major effect on the porosity of the materials, but the water content of the materials decreased with increasing dialdehyde chitosan content in subjected matrices. Briefly, a drop in proliferation was noticed for scaffolds containing 20DAC/80Coll compared to matrices with collagen alone. Furthermore, increased content of DAC (50DAC/50Coll) either significantly induced the proliferation rate or maintains its ratio compared to the control matrix. This delivery is a promising technique for additional explorations targeting therapies in regenerative dermatology. The using of dialdehyde chitosan as one of the main scaffolds components is the novelty in terms of bioengineering.https://www.mdpi.com/2073-4360/14/9/1818chitosan dialdehydecollagenbioengineeringcutaneous cellsscaffolds |
| spellingShingle | Sylwia Grabska-Zielińska Judith M. Pin Beata Kaczmarek-Szczepańska Ewa Olewnik-Kruszkowska Alina Sionkowska Fernando J. Monteiro Kerstin Steinbrink Konrad Kleszczyński Scaffolds Loaded with Dialdehyde Chitosan and Collagen—Their Physico-Chemical Properties and Biological Assessment Polymers chitosan dialdehyde collagen bioengineering cutaneous cells scaffolds |
| title | Scaffolds Loaded with Dialdehyde Chitosan and Collagen—Their Physico-Chemical Properties and Biological Assessment |
| title_full | Scaffolds Loaded with Dialdehyde Chitosan and Collagen—Their Physico-Chemical Properties and Biological Assessment |
| title_fullStr | Scaffolds Loaded with Dialdehyde Chitosan and Collagen—Their Physico-Chemical Properties and Biological Assessment |
| title_full_unstemmed | Scaffolds Loaded with Dialdehyde Chitosan and Collagen—Their Physico-Chemical Properties and Biological Assessment |
| title_short | Scaffolds Loaded with Dialdehyde Chitosan and Collagen—Their Physico-Chemical Properties and Biological Assessment |
| title_sort | scaffolds loaded with dialdehyde chitosan and collagen their physico chemical properties and biological assessment |
| topic | chitosan dialdehyde collagen bioengineering cutaneous cells scaffolds |
| url | https://www.mdpi.com/2073-4360/14/9/1818 |
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