CBX7 regulates metastasis of basal-like breast cancer through Twist1/EphA2 pathway
Background: Basal-like breast cancer (BLBC) is an important subtype of breast cancer. Twist1 is a key transcription factor in BLBC metastasis, which serves a key role in tumorigenesis. The potential mechanism of Twist1 in BLBC remains to be elucidated. Here, we explored the role and molecular mechan...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Translational Oncology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1936523322001279 |
| _version_ | 1811310110194008064 |
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| author | Tao Dai Yiqi Liu Renxian Cao Jingying Cao |
| author_facet | Tao Dai Yiqi Liu Renxian Cao Jingying Cao |
| author_sort | Tao Dai |
| collection | DOAJ |
| description | Background: Basal-like breast cancer (BLBC) is an important subtype of breast cancer. Twist1 is a key transcription factor in BLBC metastasis, which serves a key role in tumorigenesis. The potential mechanism of Twist1 in BLBC remains to be elucidated. Here, we explored the role and molecular mechanism of Twist1 in BLBC. Methods: The levels of CBX7, Twist1 and EphA2 in BLBC tissues and cells were determined by Western blot. ChIP and dual-luciferase reporter assays confirmed the interaction between CBX7, Twist1 and EphA2 promoter. The cellular functions were analyzed by CCK-8, colony formation, wound healing and Transwell assays. Expression of EMT related proteins was analyzed by Western blot. IHC measured the expression of CBX7, Twist1 and EphA2 in tumor tissues. Results: CBX7 was down-regulated in BLBC tissues and cells, whereas Twist1 and EphA2 were up-regulated. Twist1 silencing inhibited the cell migration, invasion and cancer metastasis of BLBC through targeting EphA2 and regulating EphA2 expression. Additionally, CBX7 blocked the binding of Twist1 to EphA2 promoter and inhibited EphA2 expression and suppressed BLBC growth and metastasis via Twist1/EphA2 axis. Conclusion: CBX7 suppresses BLBC growth and metastasis through Twist1/EphA2 pathway. Our study may provide evidence and new therapeutic targets for the comprehensive treatment of BLBC. |
| first_indexed | 2024-04-13T09:53:45Z |
| format | Article |
| id | doaj.art-5b9e170052684b68b1648fed02a54bc0 |
| institution | Directory Open Access Journal |
| issn | 1936-5233 |
| language | English |
| last_indexed | 2024-04-13T09:53:45Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Translational Oncology |
| spelling | doaj.art-5b9e170052684b68b1648fed02a54bc02022-12-22T02:51:31ZengElsevierTranslational Oncology1936-52332022-10-0124101468CBX7 regulates metastasis of basal-like breast cancer through Twist1/EphA2 pathwayTao Dai0Yiqi Liu1Renxian Cao2Jingying Cao3Department of Urology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Hunan Cancer Hospital, Changsha 410013 Hunan Province, PR ChinaThe Second Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, PR ChinaInstitute of Clinical Medicine, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, PR ChinaDepartment of Medicine Clinical Laboratory, The Third Xiangya Hospital of Central South University, Changsha 410013 Hunan Province, PR China; Corresponding author: 138 Tongzipo Road, Changsha 410013 Hunan Province, PR ChinaBackground: Basal-like breast cancer (BLBC) is an important subtype of breast cancer. Twist1 is a key transcription factor in BLBC metastasis, which serves a key role in tumorigenesis. The potential mechanism of Twist1 in BLBC remains to be elucidated. Here, we explored the role and molecular mechanism of Twist1 in BLBC. Methods: The levels of CBX7, Twist1 and EphA2 in BLBC tissues and cells were determined by Western blot. ChIP and dual-luciferase reporter assays confirmed the interaction between CBX7, Twist1 and EphA2 promoter. The cellular functions were analyzed by CCK-8, colony formation, wound healing and Transwell assays. Expression of EMT related proteins was analyzed by Western blot. IHC measured the expression of CBX7, Twist1 and EphA2 in tumor tissues. Results: CBX7 was down-regulated in BLBC tissues and cells, whereas Twist1 and EphA2 were up-regulated. Twist1 silencing inhibited the cell migration, invasion and cancer metastasis of BLBC through targeting EphA2 and regulating EphA2 expression. Additionally, CBX7 blocked the binding of Twist1 to EphA2 promoter and inhibited EphA2 expression and suppressed BLBC growth and metastasis via Twist1/EphA2 axis. Conclusion: CBX7 suppresses BLBC growth and metastasis through Twist1/EphA2 pathway. Our study may provide evidence and new therapeutic targets for the comprehensive treatment of BLBC.http://www.sciencedirect.com/science/article/pii/S1936523322001279Basal-like breast cancerCBX7EphA2MetastasisTwist1 |
| spellingShingle | Tao Dai Yiqi Liu Renxian Cao Jingying Cao CBX7 regulates metastasis of basal-like breast cancer through Twist1/EphA2 pathway Translational Oncology Basal-like breast cancer CBX7 EphA2 Metastasis Twist1 |
| title | CBX7 regulates metastasis of basal-like breast cancer through Twist1/EphA2 pathway |
| title_full | CBX7 regulates metastasis of basal-like breast cancer through Twist1/EphA2 pathway |
| title_fullStr | CBX7 regulates metastasis of basal-like breast cancer through Twist1/EphA2 pathway |
| title_full_unstemmed | CBX7 regulates metastasis of basal-like breast cancer through Twist1/EphA2 pathway |
| title_short | CBX7 regulates metastasis of basal-like breast cancer through Twist1/EphA2 pathway |
| title_sort | cbx7 regulates metastasis of basal like breast cancer through twist1 epha2 pathway |
| topic | Basal-like breast cancer CBX7 EphA2 Metastasis Twist1 |
| url | http://www.sciencedirect.com/science/article/pii/S1936523322001279 |
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