Macrophage Depletion Protects against Cigarette Smoke-Induced Inflammatory Response in the Mouse Colon and Lung
Cigarette smoke (CS) is considered as a major risk factor for pulmonary and intestinal inflammation. CS leads to macrophage infiltration in the mucosae of the lung and colon, inducing the uncontrolled secretion of inflammatory mediators, and thus promoting inflammatory response. In this study, we in...
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Frontiers Media S.A.
2018-02-01
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| Series: | Frontiers in Physiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fphys.2018.00047/full |
| _version_ | 1818486227407470592 |
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| author | Dahae Lim Woogyeong Kim Chanju Lee Hyunsu Bae Jinju Kim |
| author_facet | Dahae Lim Woogyeong Kim Chanju Lee Hyunsu Bae Jinju Kim |
| author_sort | Dahae Lim |
| collection | DOAJ |
| description | Cigarette smoke (CS) is considered as a major risk factor for pulmonary and intestinal inflammation. CS leads to macrophage infiltration in the mucosae of the lung and colon, inducing the uncontrolled secretion of inflammatory mediators, and thus promoting inflammatory response. In this study, we investigated whether macrophage depletion modulates cigarette smoke (CS)-induced inflammatory response in both the lung and colon. The mice were exposed to CS for 30 min, after which they were rested in a fresh air environment for 30 min. The total duration of exposure to CS was 2 h per day for 4 weeks. Macrophage depletion state was made with the injection of clodronate containing liposome. Individual body weights were measured twice a week, and the mice were sacrificed on day 28. Hematoxylin and eosin (H&E) staining was performed in the lung and colon tissue to determine histological changes. Inflammatory mediators' synthesis was analyzed using ELISA and western blotting. Clodronate liposome treatment ameliorated pathological changes associated with the infiltration of immune cells in the lung and colon. Also, clodronate liposome injected mice showed significantly lower level of inflammatory mediators, including cytokines and chemokine and proteases. Our results indicated that macrophage depletion by clodronate liposome treatment attenuates CS-induced inflammatory response in both the lung and colon. |
| first_indexed | 2024-12-10T16:20:07Z |
| format | Article |
| id | doaj.art-5b9fa4c7db394f239433666e7455cb31 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-10T16:20:07Z |
| publishDate | 2018-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Physiology |
| spelling | doaj.art-5b9fa4c7db394f239433666e7455cb312022-12-22T01:41:52ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2018-02-01910.3389/fphys.2018.00047309367Macrophage Depletion Protects against Cigarette Smoke-Induced Inflammatory Response in the Mouse Colon and LungDahae Lim0Woogyeong Kim1Chanju Lee2Hyunsu Bae3Jinju Kim4Department of Korean Physiology, College of Pharmacy, Kyung Hee University, Seoul, South KoreaDepartment of Korean Physiology, College of Pharmacy, Kyung Hee University, Seoul, South KoreaDepartment of Science in Korean Medicine, Kyung Hee University, Seoul, South KoreaDepartment of Science in Korean Medicine, Kyung Hee University, Seoul, South KoreaDepartment of Korean Physiology, College of Pharmacy, Kyung Hee University, Seoul, South KoreaCigarette smoke (CS) is considered as a major risk factor for pulmonary and intestinal inflammation. CS leads to macrophage infiltration in the mucosae of the lung and colon, inducing the uncontrolled secretion of inflammatory mediators, and thus promoting inflammatory response. In this study, we investigated whether macrophage depletion modulates cigarette smoke (CS)-induced inflammatory response in both the lung and colon. The mice were exposed to CS for 30 min, after which they were rested in a fresh air environment for 30 min. The total duration of exposure to CS was 2 h per day for 4 weeks. Macrophage depletion state was made with the injection of clodronate containing liposome. Individual body weights were measured twice a week, and the mice were sacrificed on day 28. Hematoxylin and eosin (H&E) staining was performed in the lung and colon tissue to determine histological changes. Inflammatory mediators' synthesis was analyzed using ELISA and western blotting. Clodronate liposome treatment ameliorated pathological changes associated with the infiltration of immune cells in the lung and colon. Also, clodronate liposome injected mice showed significantly lower level of inflammatory mediators, including cytokines and chemokine and proteases. Our results indicated that macrophage depletion by clodronate liposome treatment attenuates CS-induced inflammatory response in both the lung and colon.http://journal.frontiersin.org/article/10.3389/fphys.2018.00047/fullcigarette smokeclodronate liposomeintestinal inflammationmacrophagepulmonary inflammation |
| spellingShingle | Dahae Lim Woogyeong Kim Chanju Lee Hyunsu Bae Jinju Kim Macrophage Depletion Protects against Cigarette Smoke-Induced Inflammatory Response in the Mouse Colon and Lung Frontiers in Physiology cigarette smoke clodronate liposome intestinal inflammation macrophage pulmonary inflammation |
| title | Macrophage Depletion Protects against Cigarette Smoke-Induced Inflammatory Response in the Mouse Colon and Lung |
| title_full | Macrophage Depletion Protects against Cigarette Smoke-Induced Inflammatory Response in the Mouse Colon and Lung |
| title_fullStr | Macrophage Depletion Protects against Cigarette Smoke-Induced Inflammatory Response in the Mouse Colon and Lung |
| title_full_unstemmed | Macrophage Depletion Protects against Cigarette Smoke-Induced Inflammatory Response in the Mouse Colon and Lung |
| title_short | Macrophage Depletion Protects against Cigarette Smoke-Induced Inflammatory Response in the Mouse Colon and Lung |
| title_sort | macrophage depletion protects against cigarette smoke induced inflammatory response in the mouse colon and lung |
| topic | cigarette smoke clodronate liposome intestinal inflammation macrophage pulmonary inflammation |
| url | http://journal.frontiersin.org/article/10.3389/fphys.2018.00047/full |
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