Rapid replacement by non-vaccine pneumococcal serotypes may mitigate the impact of the pneumococcal conjugate vaccine on nasopharyngeal bacterial ecology
Abstract There is growing concern that interventions that alter microbial ecology can adversely affect health. We characterised the impact of the seven-valent pneumococcal conjugate vaccine (PCV7) on pneumococcal carriage and the bacterial component of the nasopharyngeal microbiome during infancy. N...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-08717-0 |
| _version_ | 1828978474169139200 |
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| author | Brenda Kwambana-Adams Blake Hanson Archibald Worwui Schadrac Agbla Ebenezer Foster-Nyarko Fatima Ceesay Chinelo Ebruke Uzochukwu Egere Yanjiao Zhou Maze Ndukum Erica Sodergren Michael Barer Richard Adegbola George Weinstock Martin Antonio |
| author_facet | Brenda Kwambana-Adams Blake Hanson Archibald Worwui Schadrac Agbla Ebenezer Foster-Nyarko Fatima Ceesay Chinelo Ebruke Uzochukwu Egere Yanjiao Zhou Maze Ndukum Erica Sodergren Michael Barer Richard Adegbola George Weinstock Martin Antonio |
| author_sort | Brenda Kwambana-Adams |
| collection | DOAJ |
| description | Abstract There is growing concern that interventions that alter microbial ecology can adversely affect health. We characterised the impact of the seven-valent pneumococcal conjugate vaccine (PCV7) on pneumococcal carriage and the bacterial component of the nasopharyngeal microbiome during infancy. Newborns were recruited into three groups as follows: Group1 (n = 33) was the control group and comprised infants who received PCV7 after 6 months and came from unvaccinated communities. Group 2 (n = 30) came from unvaccinated communities and Group 3 (n = 39) came from vaccinated communities. Both group 2 and 3 received PCV7 at 2, 3 and 4 months. Culture and 16 S rRNA gene sequencing were performed on nasopharyngeal specimens collected at regular intervals from infants. Nasopharyngeal carriage of PCV7 serotypes in Group 1 was significantly higher than in Group 2 and 3 (p < 0.01). However, pneumococcal carriage remained comparable due to an expansion of non-vaccine serotypes in Groups 2 and 3. Determination of phylogenetic dis(similarities) showed that the bacterial community structures were comparable across groups. A mixed effects model showed no difference in community richness (p = 0.15) and Shannon α-diversity (p = 0.48) across the groups. Immediate replacement of pneumococcal vaccine serotypes with non-vaccine serotypes may mitigate the impact of PCV7 on nasopharyngeal bacterial community structure and ecology. |
| first_indexed | 2024-12-14T15:18:48Z |
| format | Article |
| id | doaj.art-5ba195074d474c23abecc345fe5659b3 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T15:18:48Z |
| publishDate | 2017-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-5ba195074d474c23abecc345fe5659b32022-12-21T22:56:14ZengNature PortfolioScientific Reports2045-23222017-08-017111110.1038/s41598-017-08717-0Rapid replacement by non-vaccine pneumococcal serotypes may mitigate the impact of the pneumococcal conjugate vaccine on nasopharyngeal bacterial ecologyBrenda Kwambana-Adams0Blake Hanson1Archibald Worwui2Schadrac Agbla3Ebenezer Foster-Nyarko4Fatima Ceesay5Chinelo Ebruke6Uzochukwu Egere7Yanjiao Zhou8Maze Ndukum9Erica Sodergren10Michael Barer11Richard Adegbola12George Weinstock13Martin Antonio14Vaccines and Immunity Theme, Medical Research Council Unit The Gambia (MRCG)The Jackson Laboratory for Genomic MedicineVaccines and Immunity Theme, Medical Research Council Unit The Gambia (MRCG)Vaccines and Immunity Theme, Medical Research Council Unit The Gambia (MRCG)Vaccines and Immunity Theme, Medical Research Council Unit The Gambia (MRCG)Vaccines and Immunity Theme, Medical Research Council Unit The Gambia (MRCG)Vaccines and Immunity Theme, Medical Research Council Unit The Gambia (MRCG)Vaccines and Immunity Theme, Medical Research Council Unit The Gambia (MRCG)The Jackson Laboratory for Genomic MedicineThe Genome Instituted (Washington University in St Louis)The Jackson Laboratory for Genomic MedicineInfection, Immunity and Inflammation, University of LeicesterGSKThe Jackson Laboratory for Genomic MedicineVaccines and Immunity Theme, Medical Research Council Unit The Gambia (MRCG)Abstract There is growing concern that interventions that alter microbial ecology can adversely affect health. We characterised the impact of the seven-valent pneumococcal conjugate vaccine (PCV7) on pneumococcal carriage and the bacterial component of the nasopharyngeal microbiome during infancy. Newborns were recruited into three groups as follows: Group1 (n = 33) was the control group and comprised infants who received PCV7 after 6 months and came from unvaccinated communities. Group 2 (n = 30) came from unvaccinated communities and Group 3 (n = 39) came from vaccinated communities. Both group 2 and 3 received PCV7 at 2, 3 and 4 months. Culture and 16 S rRNA gene sequencing were performed on nasopharyngeal specimens collected at regular intervals from infants. Nasopharyngeal carriage of PCV7 serotypes in Group 1 was significantly higher than in Group 2 and 3 (p < 0.01). However, pneumococcal carriage remained comparable due to an expansion of non-vaccine serotypes in Groups 2 and 3. Determination of phylogenetic dis(similarities) showed that the bacterial community structures were comparable across groups. A mixed effects model showed no difference in community richness (p = 0.15) and Shannon α-diversity (p = 0.48) across the groups. Immediate replacement of pneumococcal vaccine serotypes with non-vaccine serotypes may mitigate the impact of PCV7 on nasopharyngeal bacterial community structure and ecology.https://doi.org/10.1038/s41598-017-08717-0 |
| spellingShingle | Brenda Kwambana-Adams Blake Hanson Archibald Worwui Schadrac Agbla Ebenezer Foster-Nyarko Fatima Ceesay Chinelo Ebruke Uzochukwu Egere Yanjiao Zhou Maze Ndukum Erica Sodergren Michael Barer Richard Adegbola George Weinstock Martin Antonio Rapid replacement by non-vaccine pneumococcal serotypes may mitigate the impact of the pneumococcal conjugate vaccine on nasopharyngeal bacterial ecology Scientific Reports |
| title | Rapid replacement by non-vaccine pneumococcal serotypes may mitigate the impact of the pneumococcal conjugate vaccine on nasopharyngeal bacterial ecology |
| title_full | Rapid replacement by non-vaccine pneumococcal serotypes may mitigate the impact of the pneumococcal conjugate vaccine on nasopharyngeal bacterial ecology |
| title_fullStr | Rapid replacement by non-vaccine pneumococcal serotypes may mitigate the impact of the pneumococcal conjugate vaccine on nasopharyngeal bacterial ecology |
| title_full_unstemmed | Rapid replacement by non-vaccine pneumococcal serotypes may mitigate the impact of the pneumococcal conjugate vaccine on nasopharyngeal bacterial ecology |
| title_short | Rapid replacement by non-vaccine pneumococcal serotypes may mitigate the impact of the pneumococcal conjugate vaccine on nasopharyngeal bacterial ecology |
| title_sort | rapid replacement by non vaccine pneumococcal serotypes may mitigate the impact of the pneumococcal conjugate vaccine on nasopharyngeal bacterial ecology |
| url | https://doi.org/10.1038/s41598-017-08717-0 |
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