Molecular analysis of alternative transcripts of equine AXL receptor tyrosine kinase gene
Objective Since athletic performance is a most importance trait in horses, most research focused on physiological and physical studies of horse athletic abilities. In contrast, the molecular analysis as well as the regulatory pathway studies remain insufficient for evaluation and prediction of horse...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2017-10-01
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| Series: | Asian-Australasian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | http://www.ajas.info/upload/pdf/ajas-30-10-1471.pdf |
| _version_ | 1831517916811493376 |
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| author | Jeong-Woong Park Ki-Duk Song Nam Young Kim Jae-Young Choi Seul A Hong Jin Hyeog Oh Si Won Kim Jeong Hyo Lee Tae Sub Park Jin-Kyoo Kim Jong Geun Kim Byung-Wook Cho |
| author_facet | Jeong-Woong Park Ki-Duk Song Nam Young Kim Jae-Young Choi Seul A Hong Jin Hyeog Oh Si Won Kim Jeong Hyo Lee Tae Sub Park Jin-Kyoo Kim Jong Geun Kim Byung-Wook Cho |
| author_sort | Jeong-Woong Park |
| collection | DOAJ |
| description | Objective Since athletic performance is a most importance trait in horses, most research focused on physiological and physical studies of horse athletic abilities. In contrast, the molecular analysis as well as the regulatory pathway studies remain insufficient for evaluation and prediction of horse athletic abilities. In our previous study, we identified AXL receptor tyrosine kinase (AXL) gene which was expressed as alternative spliced isoforms in skeletal muscle during exercise. In the present study, we validated two AXL alternative splicing transcripts (named as AXLa for long form and AXLb for short form) in equine skeletal muscle to gain insight(s) into the role of each alternative transcript during exercise. Methods We validated two isoforms of AXL transcripts in horse tissues by reverse transcriptase polymerase chain reaction (RT-PCR), and then cloned the transcripts to confirm the alternative locus and its sequences. Additionally, we examined the expression patterns of AXLa and AXLb transcripts in horse tissues by quantitative RT-PCR (qRT-PCR). Results Both of AXLa and AXLb transcripts were expressed in horse skeletal muscle and the expression levels were significantly increased after exercise. The sequencing analysis showed that there was an alternative splicing event at exon 11 between AXLa and AXLb transcripts. 3-dimentional (3D) prediction of the alternative protein structures revealed that the structural distance of the connective region between fibronectin type 3 (FN3) and immunoglobin (Ig) domain was different between two alternative isoforms. Conclusion It is assumed that the expression patterns of AXLa and AXLb transcripts would be involved in regulation of exercise-induced stress in horse muscle possibly through an NF-κB signaling pathway. Further study is necessary to uncover biological function(s) and significance of the alternative splicing isoforms in race horse skeletal muscle. |
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| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-12-12T02:48:41Z |
| publishDate | 2017-10-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-5ba3d5a895a245c69beaaaeaed9c9cb22022-12-22T00:40:56ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172017-10-0130101471147710.5713/ajas.17.040923840Molecular analysis of alternative transcripts of equine AXL receptor tyrosine kinase geneJeong-Woong Park0Ki-Duk Song1Nam Young Kim2Jae-Young Choi3Seul A Hong4Jin Hyeog Oh5Si Won Kim6Jeong Hyo Lee7Tae Sub Park8Jin-Kyoo Kim9Jong Geun Kim10Byung-Wook Cho11 Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University, Miryang 50463, Korea Department of Animal Biotechnology, Chonbuk National, University, Jeonju 54896, Korea National Institute of Animal Science, Rural Development Administration, Jeju 63242, Korea Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University, Miryang 50463, Korea Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University, Miryang 50463, Korea Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University, Miryang 50463, Korea Graduate School of International Agricultural Technology and Institute of Green-Bio Science and Technology, Seoul National University, Pyeongchang 25354, Korea Graduate School of International Agricultural Technology and Institute of Green-Bio Science and Technology, Seoul National University, Pyeongchang 25354, Korea Graduate School of International Agricultural Technology and Institute of Green-Bio Science and Technology, Seoul National University, Pyeongchang 25354, Korea Department of Microbiology, College of Natural Sciences, Changwon National University, Changwon 51140, Korea Graduate School of International Agricultural Technology and Institute of Green-Bio Science and Technology, Seoul National University, Pyeongchang 25354, Korea Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University, Miryang 50463, KoreaObjective Since athletic performance is a most importance trait in horses, most research focused on physiological and physical studies of horse athletic abilities. In contrast, the molecular analysis as well as the regulatory pathway studies remain insufficient for evaluation and prediction of horse athletic abilities. In our previous study, we identified AXL receptor tyrosine kinase (AXL) gene which was expressed as alternative spliced isoforms in skeletal muscle during exercise. In the present study, we validated two AXL alternative splicing transcripts (named as AXLa for long form and AXLb for short form) in equine skeletal muscle to gain insight(s) into the role of each alternative transcript during exercise. Methods We validated two isoforms of AXL transcripts in horse tissues by reverse transcriptase polymerase chain reaction (RT-PCR), and then cloned the transcripts to confirm the alternative locus and its sequences. Additionally, we examined the expression patterns of AXLa and AXLb transcripts in horse tissues by quantitative RT-PCR (qRT-PCR). Results Both of AXLa and AXLb transcripts were expressed in horse skeletal muscle and the expression levels were significantly increased after exercise. The sequencing analysis showed that there was an alternative splicing event at exon 11 between AXLa and AXLb transcripts. 3-dimentional (3D) prediction of the alternative protein structures revealed that the structural distance of the connective region between fibronectin type 3 (FN3) and immunoglobin (Ig) domain was different between two alternative isoforms. Conclusion It is assumed that the expression patterns of AXLa and AXLb transcripts would be involved in regulation of exercise-induced stress in horse muscle possibly through an NF-κB signaling pathway. Further study is necessary to uncover biological function(s) and significance of the alternative splicing isoforms in race horse skeletal muscle.http://www.ajas.info/upload/pdf/ajas-30-10-1471.pdfHorseAXL Receptor Tyrosine KinaseAlternative SplicingAthletic PerformanceMuscleRNA-Sequence |
| spellingShingle | Jeong-Woong Park Ki-Duk Song Nam Young Kim Jae-Young Choi Seul A Hong Jin Hyeog Oh Si Won Kim Jeong Hyo Lee Tae Sub Park Jin-Kyoo Kim Jong Geun Kim Byung-Wook Cho Molecular analysis of alternative transcripts of equine AXL receptor tyrosine kinase gene Asian-Australasian Journal of Animal Sciences Horse AXL Receptor Tyrosine Kinase Alternative Splicing Athletic Performance Muscle RNA-Sequence |
| title | Molecular analysis of alternative transcripts of equine AXL receptor tyrosine kinase gene |
| title_full | Molecular analysis of alternative transcripts of equine AXL receptor tyrosine kinase gene |
| title_fullStr | Molecular analysis of alternative transcripts of equine AXL receptor tyrosine kinase gene |
| title_full_unstemmed | Molecular analysis of alternative transcripts of equine AXL receptor tyrosine kinase gene |
| title_short | Molecular analysis of alternative transcripts of equine AXL receptor tyrosine kinase gene |
| title_sort | molecular analysis of alternative transcripts of equine axl receptor tyrosine kinase gene |
| topic | Horse AXL Receptor Tyrosine Kinase Alternative Splicing Athletic Performance Muscle RNA-Sequence |
| url | http://www.ajas.info/upload/pdf/ajas-30-10-1471.pdf |
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