Long-Term Biogas Production from Glycolate by Diverse and Highly Dynamic Communities
Generating chemical energy carriers and bulk chemicals from solar energy by microbial metabolic capacities is a promising technology. In this long-term study of over 500 days, methane was produced by a microbial community that was fed by the mono-substrate glycolate, which was derived from engineere...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-10-01
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| Series: | Microorganisms |
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| Online Access: | http://www.mdpi.com/2076-2607/6/4/103 |
| _version_ | 1828200668382363648 |
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| author | Susanne Günther Daniela Becker Thomas Hübschmann Susann Reinert Sabine Kleinsteuber Susann Müller Christian Wilhelm |
| author_facet | Susanne Günther Daniela Becker Thomas Hübschmann Susann Reinert Sabine Kleinsteuber Susann Müller Christian Wilhelm |
| author_sort | Susanne Günther |
| collection | DOAJ |
| description | Generating chemical energy carriers and bulk chemicals from solar energy by microbial metabolic capacities is a promising technology. In this long-term study of over 500 days, methane was produced by a microbial community that was fed by the mono-substrate glycolate, which was derived from engineered algae. The microbial community structure was measured on the single cell level using flow cytometry. Abiotic and operational reactor parameters were analyzed in parallel. The R-based tool flowCyBar facilitated visualization of community dynamics and indicated sub-communities involved in glycolate fermentation and methanogenesis. Cell sorting and amplicon sequencing of 16S rRNA and mcrA genes were used to identify the key organisms involved in the anaerobic conversion process. The microbial community allowed a constant fermentation, although it was sensitive to high glycolate concentrations in the feed. A linear correlation between glycolate loading rate and biogas amount was observed (R2 = 0.99) for glycolate loading rates up to 1.81 g L−1 day−1 with a maximum in biogas amount of 3635 mL day−1 encompassing 45% methane. The cytometric diversity remained high during the whole cultivation period. The dominating bacterial genera were Syntrophobotulus, Clostridia genus B55_F, Aminobacterium, and Petrimonas. Methanogenesis was almost exclusively performed by the hydrogenotrophic genus Methanobacterium. |
| first_indexed | 2024-04-12T11:16:45Z |
| format | Article |
| id | doaj.art-5ba5a970e4f944559fa3ee361946e04b |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-04-12T11:16:45Z |
| publishDate | 2018-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-5ba5a970e4f944559fa3ee361946e04b2022-12-22T03:35:28ZengMDPI AGMicroorganisms2076-26072018-10-016410310.3390/microorganisms6040103microorganisms6040103Long-Term Biogas Production from Glycolate by Diverse and Highly Dynamic CommunitiesSusanne Günther0Daniela Becker1Thomas Hübschmann2Susann Reinert3Sabine Kleinsteuber4Susann Müller5Christian Wilhelm6UFZ-Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Permoserstraße 15, 04318 Leipzig, GermanyUFZ-Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Permoserstraße 15, 04318 Leipzig, GermanyUFZ-Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Permoserstraße 15, 04318 Leipzig, GermanyInstitute of Biology, University of Leipzig, Johannisallee 21–23, 04103 Leipzig, GermanyUFZ-Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Permoserstraße 15, 04318 Leipzig, GermanyUFZ-Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Permoserstraße 15, 04318 Leipzig, GermanyInstitute of Biology, University of Leipzig, Johannisallee 21–23, 04103 Leipzig, GermanyGenerating chemical energy carriers and bulk chemicals from solar energy by microbial metabolic capacities is a promising technology. In this long-term study of over 500 days, methane was produced by a microbial community that was fed by the mono-substrate glycolate, which was derived from engineered algae. The microbial community structure was measured on the single cell level using flow cytometry. Abiotic and operational reactor parameters were analyzed in parallel. The R-based tool flowCyBar facilitated visualization of community dynamics and indicated sub-communities involved in glycolate fermentation and methanogenesis. Cell sorting and amplicon sequencing of 16S rRNA and mcrA genes were used to identify the key organisms involved in the anaerobic conversion process. The microbial community allowed a constant fermentation, although it was sensitive to high glycolate concentrations in the feed. A linear correlation between glycolate loading rate and biogas amount was observed (R2 = 0.99) for glycolate loading rates up to 1.81 g L−1 day−1 with a maximum in biogas amount of 3635 mL day−1 encompassing 45% methane. The cytometric diversity remained high during the whole cultivation period. The dominating bacterial genera were Syntrophobotulus, Clostridia genus B55_F, Aminobacterium, and Petrimonas. Methanogenesis was almost exclusively performed by the hydrogenotrophic genus Methanobacterium.http://www.mdpi.com/2076-2607/6/4/103microbial community dynamicsmicrobial flow cytometryanaerobic digestionglycolate-fermenting bacteriamethanogenic archaea |
| spellingShingle | Susanne Günther Daniela Becker Thomas Hübschmann Susann Reinert Sabine Kleinsteuber Susann Müller Christian Wilhelm Long-Term Biogas Production from Glycolate by Diverse and Highly Dynamic Communities Microorganisms microbial community dynamics microbial flow cytometry anaerobic digestion glycolate-fermenting bacteria methanogenic archaea |
| title | Long-Term Biogas Production from Glycolate by Diverse and Highly Dynamic Communities |
| title_full | Long-Term Biogas Production from Glycolate by Diverse and Highly Dynamic Communities |
| title_fullStr | Long-Term Biogas Production from Glycolate by Diverse and Highly Dynamic Communities |
| title_full_unstemmed | Long-Term Biogas Production from Glycolate by Diverse and Highly Dynamic Communities |
| title_short | Long-Term Biogas Production from Glycolate by Diverse and Highly Dynamic Communities |
| title_sort | long term biogas production from glycolate by diverse and highly dynamic communities |
| topic | microbial community dynamics microbial flow cytometry anaerobic digestion glycolate-fermenting bacteria methanogenic archaea |
| url | http://www.mdpi.com/2076-2607/6/4/103 |
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