Rational design for high bioorthogonal fluorogenicity of tetrazine‐encoded green fluorescent proteins

Rational design for high bioorthogonal fluorogenicity of tetrazine‐encoded green fluorescent proteins

Abstract The development of bioorthogonal fluorogenic probes constitutes a vital force to advance life sciences. Tetrazine‐encoded green fluorescent proteins (GFPs) show high bioorthogonal reaction rate and genetic encodability but suffer from low fluorogenicity. Here, we unveil the real‐time fluore...

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Bibliographic Details
Main Authors: Longteng Tang, Riley M. Bednar, Nikita D. Rozanov, Marcus L. Hemshorn, Ryan A. Mehl, Chong Fang
Format: Article
Language:English
Published: Wiley-VCH 2022-10-01
Series:Natural Sciences
Subjects:
bioorthogonal toolkit
femtosecond laser spectroscopy
genetic code expansion
rational protein design
turn‐on fluorescence
ultrafast energy transfer
Online Access:https://doi.org/10.1002/ntls.20220028

Internet

https://doi.org/10.1002/ntls.20220028

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