Identification of Reliable Reference Genes under Different Stresses and in Different Tissues of <i>Toxicodendron succedaneum</i>
<i>Toxicodendron succedaneum</i> (L.) Kuntze (<i>T. succedaneum</i>) is an economic tree species that produces urushiol and urushi wax, and it is of great value in industry and medicine. However, the stability of reference genes (RGs) has not been systematically reported in &...
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MDPI AG
2022-12-01
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| Series: | Genes |
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| author | Dongxiao Ma Qin Zhang Jintao Zhou Yu Lu Xiaomeng Duan Chengzhong He Jinde Yu |
| author_facet | Dongxiao Ma Qin Zhang Jintao Zhou Yu Lu Xiaomeng Duan Chengzhong He Jinde Yu |
| author_sort | Dongxiao Ma |
| collection | DOAJ |
| description | <i>Toxicodendron succedaneum</i> (L.) Kuntze (<i>T. succedaneum</i>) is an economic tree species that produces urushiol and urushi wax, and it is of great value in industry and medicine. However, the stability of reference genes (RGs) has not been systematically reported in <i>T. succedaneum</i> to date. In this study, the expression of 10 candidate RGs was analyzed by RT-qPCR in different tissues (roots, stems, leaves), stress treatments (high/low temperature, drought), and hormone stimulation (jasmonic acid, JA). Then, the stability ranking of 10 candidate genes was evaluated by ∆Ct analysis and three software programs: geNorm, NormFinder, and BestKeeper. Finally, RefFinder was used to comprehensively analyze the expression stability of 10 candidate genes. The comprehensive analysis showed that <i>TsRG05/06</i>, <i>TsRG01/06</i>, and <i>TsRG03/ACT</i> were stable under high/low-temperature stress, drought stress, and JA treatment, respectively. <i>TsRG03</i> and <i>ACT</i> had stable expression in different tissues. While the <i>TsRG03</i> and <i>ACT</i> were recommended as the suitable RGs for <i>T. succedaneum</i> in all samples. Meanwhile, <i>UBQ</i> was the least suitable as a reference gene for <i>T. succedaneum</i>. In addition, the results of geNorm showed that the combination of two stable RGs could make the results of gene expression more accurate. These results provide alternative RGs for the study of gene function, correction, and normalization of target gene expression and directed molecular breeding in <i>T. succedaneum</i>. |
| first_indexed | 2024-03-09T16:27:24Z |
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| last_indexed | 2024-03-09T16:27:24Z |
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| series | Genes |
| spelling | doaj.art-5bb427d17c024f4da59343001aae45022023-11-24T15:06:16ZengMDPI AGGenes2073-44252022-12-011312239610.3390/genes13122396Identification of Reliable Reference Genes under Different Stresses and in Different Tissues of <i>Toxicodendron succedaneum</i>Dongxiao Ma0Qin Zhang1Jintao Zhou2Yu Lu3Xiaomeng Duan4Chengzhong He5Jinde Yu6College of Life Sciences, Southwest Forestry University, Kunming 650224, ChinaCollege of Life Sciences, Southwest Forestry University, Kunming 650224, ChinaCollege of Life Sciences, Southwest Forestry University, Kunming 650224, ChinaCollege of Life Sciences, Southwest Forestry University, Kunming 650224, ChinaCollege of Life Sciences, Southwest Forestry University, Kunming 650224, ChinaCollege of Life Sciences, Southwest Forestry University, Kunming 650224, ChinaCollege of Life Sciences, Southwest Forestry University, Kunming 650224, China<i>Toxicodendron succedaneum</i> (L.) Kuntze (<i>T. succedaneum</i>) is an economic tree species that produces urushiol and urushi wax, and it is of great value in industry and medicine. However, the stability of reference genes (RGs) has not been systematically reported in <i>T. succedaneum</i> to date. In this study, the expression of 10 candidate RGs was analyzed by RT-qPCR in different tissues (roots, stems, leaves), stress treatments (high/low temperature, drought), and hormone stimulation (jasmonic acid, JA). Then, the stability ranking of 10 candidate genes was evaluated by ∆Ct analysis and three software programs: geNorm, NormFinder, and BestKeeper. Finally, RefFinder was used to comprehensively analyze the expression stability of 10 candidate genes. The comprehensive analysis showed that <i>TsRG05/06</i>, <i>TsRG01/06</i>, and <i>TsRG03/ACT</i> were stable under high/low-temperature stress, drought stress, and JA treatment, respectively. <i>TsRG03</i> and <i>ACT</i> had stable expression in different tissues. While the <i>TsRG03</i> and <i>ACT</i> were recommended as the suitable RGs for <i>T. succedaneum</i> in all samples. Meanwhile, <i>UBQ</i> was the least suitable as a reference gene for <i>T. succedaneum</i>. In addition, the results of geNorm showed that the combination of two stable RGs could make the results of gene expression more accurate. These results provide alternative RGs for the study of gene function, correction, and normalization of target gene expression and directed molecular breeding in <i>T. succedaneum</i>.https://www.mdpi.com/2073-4425/13/12/2396gene expressionreference geneRT-qPCR<i>Toxicodendron succedaneum</i> |
| spellingShingle | Dongxiao Ma Qin Zhang Jintao Zhou Yu Lu Xiaomeng Duan Chengzhong He Jinde Yu Identification of Reliable Reference Genes under Different Stresses and in Different Tissues of <i>Toxicodendron succedaneum</i> Genes gene expression reference gene RT-qPCR <i>Toxicodendron succedaneum</i> |
| title | Identification of Reliable Reference Genes under Different Stresses and in Different Tissues of <i>Toxicodendron succedaneum</i> |
| title_full | Identification of Reliable Reference Genes under Different Stresses and in Different Tissues of <i>Toxicodendron succedaneum</i> |
| title_fullStr | Identification of Reliable Reference Genes under Different Stresses and in Different Tissues of <i>Toxicodendron succedaneum</i> |
| title_full_unstemmed | Identification of Reliable Reference Genes under Different Stresses and in Different Tissues of <i>Toxicodendron succedaneum</i> |
| title_short | Identification of Reliable Reference Genes under Different Stresses and in Different Tissues of <i>Toxicodendron succedaneum</i> |
| title_sort | identification of reliable reference genes under different stresses and in different tissues of i toxicodendron succedaneum i |
| topic | gene expression reference gene RT-qPCR <i>Toxicodendron succedaneum</i> |
| url | https://www.mdpi.com/2073-4425/13/12/2396 |
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