Biogenic synthesis, characterization, antibacterial and antioxidant activities of silver nanoparticles mediated from Tamarindus indica Linn fruit pulp extract
Introduction: Due to the numerous potentials discovered from biologically synthesized silver nanoparticles (SNPs), the interest of many researchers has been stirred up. Tamarindus indica fruit has many therapeutic potentials attributed to fruit. Hence, the objective of this study was to synthesize,...
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| Format: | Article |
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Shahrekord University of Medical Sciences
2023-09-01
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| Series: | Journal of HerbMed Pharmacology |
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| Online Access: | http://herbmedpharmacol.com/PDF/jhp-12-459.pdf |
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| author | Kehinde Oluwakemi Fagbemi Olufunmiso Olusola Olajuyigbe Roger Coopoosamy |
| author_facet | Kehinde Oluwakemi Fagbemi Olufunmiso Olusola Olajuyigbe Roger Coopoosamy |
| author_sort | Kehinde Oluwakemi Fagbemi |
| collection | DOAJ |
| description | Introduction: Due to the numerous potentials discovered from biologically synthesized silver nanoparticles (SNPs), the interest of many researchers has been stirred up. Tamarindus indica fruit has many therapeutic potentials attributed to fruit. Hence, the objective of this study was to synthesize, characterize, and evaluate the in vitro antioxidant and antibacterial activities of SNPs mediated from T. indica fruit pulp extract. Methods: The bioreduction of silver nitrate was performed using methanol extract of T. indica fruit pulp. UV–vis spectrophotometry studies at 480 nm confirmed the synthesis of SNPs. The synthesized nanoparticles were characterized using Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), and energy-dispersive X-ray spectroscopy (EDX). The antioxidant properties were assessed using methods of ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). The antibacterial potential was evaluated using the agar well technique. Results: FTIR spectroscopy revealed that the presence of various functional groups was responsible for reduction and stabilization during the biosynthesis process. With the aid of ImageJ software, the size of the nanoparticles was determined to be in the range of 18-50 nm. The anti-oxidation activity assays showed a strong reducing potential towards the radicals tested. Lastly, strong antibacterial activities were observed when the nanoparticles were tested on some pathogenic bacteria through the agar well method. Conclusion: This biological method of synthesizing SNP from T. indica has shown significant enhancement in its biological activities in terms of antibacterial and antioxidant properties; thus, it might be considered a therapeutic agent. |
| first_indexed | 2024-03-09T15:38:56Z |
| format | Article |
| id | doaj.art-5bb5d457dd8c46189b0d3d528b598c11 |
| institution | Directory Open Access Journal |
| issn | 2345-5004 |
| language | English |
| last_indexed | 2024-03-09T15:38:56Z |
| publishDate | 2023-09-01 |
| publisher | Shahrekord University of Medical Sciences |
| record_format | Article |
| series | Journal of HerbMed Pharmacology |
| spelling | doaj.art-5bb5d457dd8c46189b0d3d528b598c112023-11-25T08:27:28ZengShahrekord University of Medical SciencesJournal of HerbMed Pharmacology2345-50042023-09-0112445946810.34172/jhp.2023.43430jhp-43430Biogenic synthesis, characterization, antibacterial and antioxidant activities of silver nanoparticles mediated from Tamarindus indica Linn fruit pulp extractKehinde Oluwakemi Fagbemi0Olufunmiso Olusola Olajuyigbe1Roger Coopoosamy2Department of Microbiology, School of Science Technology, PMB 4005, Babcock University, Ilisan Remo, Ogun State, NigeriaDepartment of Microbiology, School of Science Technology, PMB 4005, Babcock University, Ilisan Remo, Ogun State, NigeriaDepartment of Nature Conservation, Faculty of Natural Science, Mangosuthu University of Technology, P.O. Box 12363, Jacobs, 4026, Durban, South AfricaIntroduction: Due to the numerous potentials discovered from biologically synthesized silver nanoparticles (SNPs), the interest of many researchers has been stirred up. Tamarindus indica fruit has many therapeutic potentials attributed to fruit. Hence, the objective of this study was to synthesize, characterize, and evaluate the in vitro antioxidant and antibacterial activities of SNPs mediated from T. indica fruit pulp extract. Methods: The bioreduction of silver nitrate was performed using methanol extract of T. indica fruit pulp. UV–vis spectrophotometry studies at 480 nm confirmed the synthesis of SNPs. The synthesized nanoparticles were characterized using Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), and energy-dispersive X-ray spectroscopy (EDX). The antioxidant properties were assessed using methods of ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). The antibacterial potential was evaluated using the agar well technique. Results: FTIR spectroscopy revealed that the presence of various functional groups was responsible for reduction and stabilization during the biosynthesis process. With the aid of ImageJ software, the size of the nanoparticles was determined to be in the range of 18-50 nm. The anti-oxidation activity assays showed a strong reducing potential towards the radicals tested. Lastly, strong antibacterial activities were observed when the nanoparticles were tested on some pathogenic bacteria through the agar well method. Conclusion: This biological method of synthesizing SNP from T. indica has shown significant enhancement in its biological activities in terms of antibacterial and antioxidant properties; thus, it might be considered a therapeutic agent.http://herbmedpharmacol.com/PDF/jhp-12-459.pdfbiogenic activityoxidative stressfunctional groupsilver nanoparticles |
| spellingShingle | Kehinde Oluwakemi Fagbemi Olufunmiso Olusola Olajuyigbe Roger Coopoosamy Biogenic synthesis, characterization, antibacterial and antioxidant activities of silver nanoparticles mediated from Tamarindus indica Linn fruit pulp extract Journal of HerbMed Pharmacology biogenic activity oxidative stress functional group silver nanoparticles |
| title | Biogenic synthesis, characterization, antibacterial and antioxidant activities of silver nanoparticles mediated from Tamarindus indica Linn fruit pulp extract |
| title_full | Biogenic synthesis, characterization, antibacterial and antioxidant activities of silver nanoparticles mediated from Tamarindus indica Linn fruit pulp extract |
| title_fullStr | Biogenic synthesis, characterization, antibacterial and antioxidant activities of silver nanoparticles mediated from Tamarindus indica Linn fruit pulp extract |
| title_full_unstemmed | Biogenic synthesis, characterization, antibacterial and antioxidant activities of silver nanoparticles mediated from Tamarindus indica Linn fruit pulp extract |
| title_short | Biogenic synthesis, characterization, antibacterial and antioxidant activities of silver nanoparticles mediated from Tamarindus indica Linn fruit pulp extract |
| title_sort | biogenic synthesis characterization antibacterial and antioxidant activities of silver nanoparticles mediated from tamarindus indica linn fruit pulp extract |
| topic | biogenic activity oxidative stress functional group silver nanoparticles |
| url | http://herbmedpharmacol.com/PDF/jhp-12-459.pdf |
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