Transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species Aegilops speltoides
Abstract Wheat, one of the major cereal crops worldwide, get adversely affected by rising global temperature. We have identified the diploid B genome progenitor of wheat, Aegilops speltoides (SS), as a potential donor for heat stress tolerance. Therefore, the present work was planned to study the to...
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Nature Portfolio
2021-11-01
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Online Access: | https://doi.org/10.1038/s41598-021-01596-6 |
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author | Sushmita Seni Satinder Kaur Palvi Malik Inderjit Singh Yadav Parul Sirohi Harsh Chauhan Amandeep Kaur Parveen Chhuneja |
author_facet | Sushmita Seni Satinder Kaur Palvi Malik Inderjit Singh Yadav Parul Sirohi Harsh Chauhan Amandeep Kaur Parveen Chhuneja |
author_sort | Sushmita Seni |
collection | DOAJ |
description | Abstract Wheat, one of the major cereal crops worldwide, get adversely affected by rising global temperature. We have identified the diploid B genome progenitor of wheat, Aegilops speltoides (SS), as a potential donor for heat stress tolerance. Therefore, the present work was planned to study the total transcriptome profile of heat stress-tolerant Ae. speltoides accession pau3809 (AS3809) and compare with that of tetraploid and hexaploid wheat cultivars PDW274 and PBW725, respectively. The comparative transcriptome was utilized to identify and validate heat stress transcription factors (HSFs), the key genes involved in imparting heat stress tolerance. Transcriptome analysis led to the identification of a total of 74 K, 68 K, and 76 K genes in AS3809, PDW274, and PBW725, respectively. There was a high uniformity of GO profiles under the biological, molecular, and cellular functions across the three wheat transcriptomes, suggesting the conservation of gene function. Twelve HSFs having the highest FPKM value were identified in the AS3809 transcriptome data, while six of these HSFs namely HSFA3, HSFA5, HSFA9, HSFB2a, HSFB2b, and HSFC1b, were validated with qRT PCR. These six HSFs were identified as an important component of thermotolerance in AS3809 as evident from their comparative higher expression under heat stress. |
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language | English |
last_indexed | 2024-12-20T04:39:27Z |
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spelling | doaj.art-5bb79f03f1354edfb29bfdc2db5b788b2022-12-21T19:53:10ZengNature PortfolioScientific Reports2045-23222021-11-0111111510.1038/s41598-021-01596-6Transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species Aegilops speltoidesSushmita Seni0Satinder Kaur1Palvi Malik2Inderjit Singh Yadav3Parul Sirohi4Harsh Chauhan5Amandeep Kaur6Parveen Chhuneja7School of Agricultural Biotechnology, Punjab Agricultural UniversitySchool of Agricultural Biotechnology, Punjab Agricultural UniversitySchool of Agricultural Biotechnology, Punjab Agricultural UniversitySchool of Agricultural Biotechnology, Punjab Agricultural UniversityDepartment of Biosciences and Bioengineering, Indian Institute of Technology RoorkeeDepartment of Biosciences and Bioengineering, Indian Institute of Technology RoorkeeSchool of Agricultural Biotechnology, Punjab Agricultural UniversitySchool of Agricultural Biotechnology, Punjab Agricultural UniversityAbstract Wheat, one of the major cereal crops worldwide, get adversely affected by rising global temperature. We have identified the diploid B genome progenitor of wheat, Aegilops speltoides (SS), as a potential donor for heat stress tolerance. Therefore, the present work was planned to study the total transcriptome profile of heat stress-tolerant Ae. speltoides accession pau3809 (AS3809) and compare with that of tetraploid and hexaploid wheat cultivars PDW274 and PBW725, respectively. The comparative transcriptome was utilized to identify and validate heat stress transcription factors (HSFs), the key genes involved in imparting heat stress tolerance. Transcriptome analysis led to the identification of a total of 74 K, 68 K, and 76 K genes in AS3809, PDW274, and PBW725, respectively. There was a high uniformity of GO profiles under the biological, molecular, and cellular functions across the three wheat transcriptomes, suggesting the conservation of gene function. Twelve HSFs having the highest FPKM value were identified in the AS3809 transcriptome data, while six of these HSFs namely HSFA3, HSFA5, HSFA9, HSFB2a, HSFB2b, and HSFC1b, were validated with qRT PCR. These six HSFs were identified as an important component of thermotolerance in AS3809 as evident from their comparative higher expression under heat stress.https://doi.org/10.1038/s41598-021-01596-6 |
spellingShingle | Sushmita Seni Satinder Kaur Palvi Malik Inderjit Singh Yadav Parul Sirohi Harsh Chauhan Amandeep Kaur Parveen Chhuneja Transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species Aegilops speltoides Scientific Reports |
title | Transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species Aegilops speltoides |
title_full | Transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species Aegilops speltoides |
title_fullStr | Transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species Aegilops speltoides |
title_full_unstemmed | Transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species Aegilops speltoides |
title_short | Transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species Aegilops speltoides |
title_sort | transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species aegilops speltoides |
url | https://doi.org/10.1038/s41598-021-01596-6 |
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