Transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species Aegilops speltoides

Abstract Wheat, one of the major cereal crops worldwide, get adversely affected by rising global temperature. We have identified the diploid B genome progenitor of wheat, Aegilops speltoides (SS), as a potential donor for heat stress tolerance. Therefore, the present work was planned to study the to...

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Main Authors: Sushmita Seni, Satinder Kaur, Palvi Malik, Inderjit Singh Yadav, Parul Sirohi, Harsh Chauhan, Amandeep Kaur, Parveen Chhuneja
Format: Article
Language:English
Published: Nature Portfolio 2021-11-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-01596-6
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author Sushmita Seni
Satinder Kaur
Palvi Malik
Inderjit Singh Yadav
Parul Sirohi
Harsh Chauhan
Amandeep Kaur
Parveen Chhuneja
author_facet Sushmita Seni
Satinder Kaur
Palvi Malik
Inderjit Singh Yadav
Parul Sirohi
Harsh Chauhan
Amandeep Kaur
Parveen Chhuneja
author_sort Sushmita Seni
collection DOAJ
description Abstract Wheat, one of the major cereal crops worldwide, get adversely affected by rising global temperature. We have identified the diploid B genome progenitor of wheat, Aegilops speltoides (SS), as a potential donor for heat stress tolerance. Therefore, the present work was planned to study the total transcriptome profile of heat stress-tolerant Ae. speltoides accession pau3809 (AS3809) and compare with that of tetraploid and hexaploid wheat cultivars PDW274 and PBW725, respectively. The comparative transcriptome was utilized to identify and validate heat stress transcription factors (HSFs), the key genes involved in imparting heat stress tolerance. Transcriptome analysis led to the identification of a total of 74 K, 68 K, and 76 K genes in AS3809, PDW274, and PBW725, respectively. There was a high uniformity of GO profiles under the biological, molecular, and cellular functions across the three wheat transcriptomes, suggesting the conservation of gene function. Twelve HSFs having the highest FPKM value were identified in the AS3809 transcriptome data, while six of these HSFs namely HSFA3, HSFA5, HSFA9, HSFB2a, HSFB2b, and HSFC1b, were validated with qRT PCR. These six HSFs were identified as an important component of thermotolerance in AS3809 as evident from their comparative higher expression under heat stress.
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spelling doaj.art-5bb79f03f1354edfb29bfdc2db5b788b2022-12-21T19:53:10ZengNature PortfolioScientific Reports2045-23222021-11-0111111510.1038/s41598-021-01596-6Transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species Aegilops speltoidesSushmita Seni0Satinder Kaur1Palvi Malik2Inderjit Singh Yadav3Parul Sirohi4Harsh Chauhan5Amandeep Kaur6Parveen Chhuneja7School of Agricultural Biotechnology, Punjab Agricultural UniversitySchool of Agricultural Biotechnology, Punjab Agricultural UniversitySchool of Agricultural Biotechnology, Punjab Agricultural UniversitySchool of Agricultural Biotechnology, Punjab Agricultural UniversityDepartment of Biosciences and Bioengineering, Indian Institute of Technology RoorkeeDepartment of Biosciences and Bioengineering, Indian Institute of Technology RoorkeeSchool of Agricultural Biotechnology, Punjab Agricultural UniversitySchool of Agricultural Biotechnology, Punjab Agricultural UniversityAbstract Wheat, one of the major cereal crops worldwide, get adversely affected by rising global temperature. We have identified the diploid B genome progenitor of wheat, Aegilops speltoides (SS), as a potential donor for heat stress tolerance. Therefore, the present work was planned to study the total transcriptome profile of heat stress-tolerant Ae. speltoides accession pau3809 (AS3809) and compare with that of tetraploid and hexaploid wheat cultivars PDW274 and PBW725, respectively. The comparative transcriptome was utilized to identify and validate heat stress transcription factors (HSFs), the key genes involved in imparting heat stress tolerance. Transcriptome analysis led to the identification of a total of 74 K, 68 K, and 76 K genes in AS3809, PDW274, and PBW725, respectively. There was a high uniformity of GO profiles under the biological, molecular, and cellular functions across the three wheat transcriptomes, suggesting the conservation of gene function. Twelve HSFs having the highest FPKM value were identified in the AS3809 transcriptome data, while six of these HSFs namely HSFA3, HSFA5, HSFA9, HSFB2a, HSFB2b, and HSFC1b, were validated with qRT PCR. These six HSFs were identified as an important component of thermotolerance in AS3809 as evident from their comparative higher expression under heat stress.https://doi.org/10.1038/s41598-021-01596-6
spellingShingle Sushmita Seni
Satinder Kaur
Palvi Malik
Inderjit Singh Yadav
Parul Sirohi
Harsh Chauhan
Amandeep Kaur
Parveen Chhuneja
Transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species Aegilops speltoides
Scientific Reports
title Transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species Aegilops speltoides
title_full Transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species Aegilops speltoides
title_fullStr Transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species Aegilops speltoides
title_full_unstemmed Transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species Aegilops speltoides
title_short Transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species Aegilops speltoides
title_sort transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species aegilops speltoides
url https://doi.org/10.1038/s41598-021-01596-6
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