Myosin motors fragment and compact membrane-bound actin filaments
Cell cortex remodeling during cell division is a result of myofilament-driven contractility of the cortical membrane-bound actin meshwork. Little is known about the interaction between individual myofilaments and membrane-bound actin filaments. Here we reconstituted a minimal actin cortex to directl...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2013-01-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/00116 |
| _version_ | 1818018988859326464 |
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| author | Sven K Vogel Zdenek Petrasek Fabian Heinemann Petra Schwille |
| author_facet | Sven K Vogel Zdenek Petrasek Fabian Heinemann Petra Schwille |
| author_sort | Sven K Vogel |
| collection | DOAJ |
| description | Cell cortex remodeling during cell division is a result of myofilament-driven contractility of the cortical membrane-bound actin meshwork. Little is known about the interaction between individual myofilaments and membrane-bound actin filaments. Here we reconstituted a minimal actin cortex to directly visualize the action of individual myofilaments on membrane-bound actin filaments using TIRF microscopy. We show that synthetic myofilaments fragment and compact membrane-bound actin while processively moving along actin filaments. We propose a mechanism by which tension builds up between the ends of myofilaments, resulting in compressive stress exerted to single actin filaments, causing their buckling and breakage. Modeling of this mechanism revealed that sufficient force (∼20 pN) can be generated by single myofilaments to buckle and break actin filaments. This mechanism of filament fragmentation and compaction may contribute to actin turnover and cortex reorganization during cytokinesis. |
| first_indexed | 2024-04-14T07:47:05Z |
| format | Article |
| id | doaj.art-5bbaf63094e449f1974dd672466b03e5 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-14T07:47:05Z |
| publishDate | 2013-01-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-5bbaf63094e449f1974dd672466b03e52022-12-22T02:05:19ZengeLife Sciences Publications LtdeLife2050-084X2013-01-01210.7554/eLife.00116Myosin motors fragment and compact membrane-bound actin filamentsSven K Vogel0Zdenek Petrasek1Fabian Heinemann2Petra Schwille3Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Martinsried, GermanyDepartment of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Martinsried, GermanyDepartment of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Martinsried, GermanyDepartment of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Martinsried, GermanyCell cortex remodeling during cell division is a result of myofilament-driven contractility of the cortical membrane-bound actin meshwork. Little is known about the interaction between individual myofilaments and membrane-bound actin filaments. Here we reconstituted a minimal actin cortex to directly visualize the action of individual myofilaments on membrane-bound actin filaments using TIRF microscopy. We show that synthetic myofilaments fragment and compact membrane-bound actin while processively moving along actin filaments. We propose a mechanism by which tension builds up between the ends of myofilaments, resulting in compressive stress exerted to single actin filaments, causing their buckling and breakage. Modeling of this mechanism revealed that sufficient force (∼20 pN) can be generated by single myofilaments to buckle and break actin filaments. This mechanism of filament fragmentation and compaction may contribute to actin turnover and cortex reorganization during cytokinesis.https://elifesciences.org/articles/00116MyosinActinActin CortexMyofilamentTIRFMembrane |
| spellingShingle | Sven K Vogel Zdenek Petrasek Fabian Heinemann Petra Schwille Myosin motors fragment and compact membrane-bound actin filaments eLife Myosin Actin Actin Cortex Myofilament TIRF Membrane |
| title | Myosin motors fragment and compact membrane-bound actin filaments |
| title_full | Myosin motors fragment and compact membrane-bound actin filaments |
| title_fullStr | Myosin motors fragment and compact membrane-bound actin filaments |
| title_full_unstemmed | Myosin motors fragment and compact membrane-bound actin filaments |
| title_short | Myosin motors fragment and compact membrane-bound actin filaments |
| title_sort | myosin motors fragment and compact membrane bound actin filaments |
| topic | Myosin Actin Actin Cortex Myofilament TIRF Membrane |
| url | https://elifesciences.org/articles/00116 |
| work_keys_str_mv | AT svenkvogel myosinmotorsfragmentandcompactmembraneboundactinfilaments AT zdenekpetrasek myosinmotorsfragmentandcompactmembraneboundactinfilaments AT fabianheinemann myosinmotorsfragmentandcompactmembraneboundactinfilaments AT petraschwille myosinmotorsfragmentandcompactmembraneboundactinfilaments |