The coupling of the M2 muscarinic receptor to its G protein is voltage dependent.
G protein coupled receptors (GPCRs) participate in the majority of signal transduction processes in the body. Specifically, the binding of an external agonist promotes coupling of the GPCR to its G protein and this, in turn, induces downstream signaling. Recently, it was shown that agonist binding t...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0224367 |
| _version_ | 1829502244811177984 |
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| author | Yair Ben-Chaim Chava Broide Hanna Parnas |
| author_facet | Yair Ben-Chaim Chava Broide Hanna Parnas |
| author_sort | Yair Ben-Chaim |
| collection | DOAJ |
| description | G protein coupled receptors (GPCRs) participate in the majority of signal transduction processes in the body. Specifically, the binding of an external agonist promotes coupling of the GPCR to its G protein and this, in turn, induces downstream signaling. Recently, it was shown that agonist binding to the M2 muscarinic receptor (M2R) and to other GPCRs is voltage dependent. Here we examine, whether the coupling of the M2R to its G protein is also voltage-dependent. We first show, in Xenopus oocytes, that the activity of the M2R in the absence of agonist (constitutive activity) can be used to report the coupling. We then show that the coupling is, by itself, voltage dependent. This novel finding is of physiological importance, as it shows that the actual signal transduction, whose first step is the coupling of the GPCR to its cognate G protein, is voltage dependent. |
| first_indexed | 2024-12-16T09:33:24Z |
| format | Article |
| id | doaj.art-5bbf3f96049e4b509536a56373d14bfd |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T09:33:24Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5bbf3f96049e4b509536a56373d14bfd2022-12-21T22:36:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-011410e022436710.1371/journal.pone.0224367The coupling of the M2 muscarinic receptor to its G protein is voltage dependent.Yair Ben-ChaimChava BroideHanna ParnasG protein coupled receptors (GPCRs) participate in the majority of signal transduction processes in the body. Specifically, the binding of an external agonist promotes coupling of the GPCR to its G protein and this, in turn, induces downstream signaling. Recently, it was shown that agonist binding to the M2 muscarinic receptor (M2R) and to other GPCRs is voltage dependent. Here we examine, whether the coupling of the M2R to its G protein is also voltage-dependent. We first show, in Xenopus oocytes, that the activity of the M2R in the absence of agonist (constitutive activity) can be used to report the coupling. We then show that the coupling is, by itself, voltage dependent. This novel finding is of physiological importance, as it shows that the actual signal transduction, whose first step is the coupling of the GPCR to its cognate G protein, is voltage dependent.https://doi.org/10.1371/journal.pone.0224367 |
| spellingShingle | Yair Ben-Chaim Chava Broide Hanna Parnas The coupling of the M2 muscarinic receptor to its G protein is voltage dependent. PLoS ONE |
| title | The coupling of the M2 muscarinic receptor to its G protein is voltage dependent. |
| title_full | The coupling of the M2 muscarinic receptor to its G protein is voltage dependent. |
| title_fullStr | The coupling of the M2 muscarinic receptor to its G protein is voltage dependent. |
| title_full_unstemmed | The coupling of the M2 muscarinic receptor to its G protein is voltage dependent. |
| title_short | The coupling of the M2 muscarinic receptor to its G protein is voltage dependent. |
| title_sort | coupling of the m2 muscarinic receptor to its g protein is voltage dependent |
| url | https://doi.org/10.1371/journal.pone.0224367 |
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