A genotoxicity study on Talitrus saltator (Crustacea, Amphipoda) exposed to lead and benzo(a)pyrene

Sandy beaches are ecosystems often subjected to a variety of pollution sources, including heavy metals and polycyclic aromatic hydrocarbons, largely due to improper human activities. The sandhopper Talitrus saltator s. l. (Montagu, 1808) typically lives on supralittoral shores where it plays an impo...

Full description

Bibliographic Details
Main Authors: G. Di Donato, D. Davolos, C. Chimenti, E. De Matthaeis
Format: Article
Language:English
Published: Taylor & Francis Group 2020-01-01
Series:The European Zoological Journal
Subjects:
Online Access:http://dx.doi.org/10.1080/24750263.2020.1751887
_version_ 1818441492863123456
author G. Di Donato
D. Davolos
C. Chimenti
E. De Matthaeis
author_facet G. Di Donato
D. Davolos
C. Chimenti
E. De Matthaeis
author_sort G. Di Donato
collection DOAJ
description Sandy beaches are ecosystems often subjected to a variety of pollution sources, including heavy metals and polycyclic aromatic hydrocarbons, largely due to improper human activities. The sandhopper Talitrus saltator s. l. (Montagu, 1808) typically lives on supralittoral shores where it plays an important ecological role. The purpose of this study was to investigate DNA damage levels on hemocytes of T. saltator by means of the Comet assay. Firstly, we tested the sensitivity and reproducibility of the Comet assay on hemocytes of T. saltator after in vitro exposure (1 h) to the genotoxicant methyl methanesulfonate (MMS). The data demonstrated a DNA damage (defined as % DNA tail) related to MMS exposure in a concentration-dependent manner. Next, we carried out the assay on hemocytes of T. saltator that were exposed in vivo (24 h and 7 days) to (a) lead (Pb), (b) benzo(a)pyrene (B(a)P), (c) mixtures of them. Our data on exposure to Pb or B(a)P showed DNA damage on the hemocytes of T. saltator in a concentration-dependent manner, but apparently with a non-synergistic effect of exposure to their mixtures. Our results provide a background for further studies in order to verify the applicability of Comet assay on T. saltator for assessing genotoxicity levels in sandy beaches subjected to pollution.
first_indexed 2024-12-14T18:29:07Z
format Article
id doaj.art-5bc169ea733a4b399c9beeeb86250eb2
institution Directory Open Access Journal
issn 2475-0263
language English
last_indexed 2024-12-14T18:29:07Z
publishDate 2020-01-01
publisher Taylor & Francis Group
record_format Article
series The European Zoological Journal
spelling doaj.art-5bc169ea733a4b399c9beeeb86250eb22022-12-21T22:51:50ZengTaylor & Francis GroupThe European Zoological Journal2475-02632020-01-0187123524110.1080/24750263.2020.17518871751887A genotoxicity study on Talitrus saltator (Crustacea, Amphipoda) exposed to lead and benzo(a)pyreneG. Di Donato0D. Davolos1C. Chimenti2E. De Matthaeis3Sapienza University of RomeSapienza University of RomeSapienza University of RomeSapienza University of RomeSandy beaches are ecosystems often subjected to a variety of pollution sources, including heavy metals and polycyclic aromatic hydrocarbons, largely due to improper human activities. The sandhopper Talitrus saltator s. l. (Montagu, 1808) typically lives on supralittoral shores where it plays an important ecological role. The purpose of this study was to investigate DNA damage levels on hemocytes of T. saltator by means of the Comet assay. Firstly, we tested the sensitivity and reproducibility of the Comet assay on hemocytes of T. saltator after in vitro exposure (1 h) to the genotoxicant methyl methanesulfonate (MMS). The data demonstrated a DNA damage (defined as % DNA tail) related to MMS exposure in a concentration-dependent manner. Next, we carried out the assay on hemocytes of T. saltator that were exposed in vivo (24 h and 7 days) to (a) lead (Pb), (b) benzo(a)pyrene (B(a)P), (c) mixtures of them. Our data on exposure to Pb or B(a)P showed DNA damage on the hemocytes of T. saltator in a concentration-dependent manner, but apparently with a non-synergistic effect of exposure to their mixtures. Our results provide a background for further studies in order to verify the applicability of Comet assay on T. saltator for assessing genotoxicity levels in sandy beaches subjected to pollution.http://dx.doi.org/10.1080/24750263.2020.1751887comet assaydna damagehemocytesheavy metalspolycyclic aromatic hydrocarbons
spellingShingle G. Di Donato
D. Davolos
C. Chimenti
E. De Matthaeis
A genotoxicity study on Talitrus saltator (Crustacea, Amphipoda) exposed to lead and benzo(a)pyrene
The European Zoological Journal
comet assay
dna damage
hemocytes
heavy metals
polycyclic aromatic hydrocarbons
title A genotoxicity study on Talitrus saltator (Crustacea, Amphipoda) exposed to lead and benzo(a)pyrene
title_full A genotoxicity study on Talitrus saltator (Crustacea, Amphipoda) exposed to lead and benzo(a)pyrene
title_fullStr A genotoxicity study on Talitrus saltator (Crustacea, Amphipoda) exposed to lead and benzo(a)pyrene
title_full_unstemmed A genotoxicity study on Talitrus saltator (Crustacea, Amphipoda) exposed to lead and benzo(a)pyrene
title_short A genotoxicity study on Talitrus saltator (Crustacea, Amphipoda) exposed to lead and benzo(a)pyrene
title_sort genotoxicity study on talitrus saltator crustacea amphipoda exposed to lead and benzo a pyrene
topic comet assay
dna damage
hemocytes
heavy metals
polycyclic aromatic hydrocarbons
url http://dx.doi.org/10.1080/24750263.2020.1751887
work_keys_str_mv AT gdidonato agenotoxicitystudyontalitrussaltatorcrustaceaamphipodaexposedtoleadandbenzoapyrene
AT ddavolos agenotoxicitystudyontalitrussaltatorcrustaceaamphipodaexposedtoleadandbenzoapyrene
AT cchimenti agenotoxicitystudyontalitrussaltatorcrustaceaamphipodaexposedtoleadandbenzoapyrene
AT edematthaeis agenotoxicitystudyontalitrussaltatorcrustaceaamphipodaexposedtoleadandbenzoapyrene
AT gdidonato genotoxicitystudyontalitrussaltatorcrustaceaamphipodaexposedtoleadandbenzoapyrene
AT ddavolos genotoxicitystudyontalitrussaltatorcrustaceaamphipodaexposedtoleadandbenzoapyrene
AT cchimenti genotoxicitystudyontalitrussaltatorcrustaceaamphipodaexposedtoleadandbenzoapyrene
AT edematthaeis genotoxicitystudyontalitrussaltatorcrustaceaamphipodaexposedtoleadandbenzoapyrene