Hepatic galectin-3 is associated with lipid droplet area in non-alcoholic steatohepatitis in a new swine model
Abstract Non-alcoholic fatty liver disease (NAFLD) is currently a growing epidemic disease that can lead to cirrhosis and hepatic cancer when it evolves into non-alcoholic steatohepatitis (NASH), a gap not well understood. To characterize this disease, pigs, considered to be one of the most similar...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-04971-z |
| _version_ | 1798025216000000000 |
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| author | Luis V. Herrera-Marcos Roberto Martínez-Beamonte Manuel Macías-Herranz Carmen Arnal Cristina Barranquero Juan J. Puente-Lanzarote Sonia Gascón Tania Herrero-Continente Gonzalo Gonzalo-Romeo Víctor Alastrué-Vera Dolores Gutiérrez-Blázquez José M. Lou-Bonafonte Joaquín C. Surra María J. Rodríguez-Yoldi Agustín García-Gil Antonio Güemes Jesús Osada |
| author_facet | Luis V. Herrera-Marcos Roberto Martínez-Beamonte Manuel Macías-Herranz Carmen Arnal Cristina Barranquero Juan J. Puente-Lanzarote Sonia Gascón Tania Herrero-Continente Gonzalo Gonzalo-Romeo Víctor Alastrué-Vera Dolores Gutiérrez-Blázquez José M. Lou-Bonafonte Joaquín C. Surra María J. Rodríguez-Yoldi Agustín García-Gil Antonio Güemes Jesús Osada |
| author_sort | Luis V. Herrera-Marcos |
| collection | DOAJ |
| description | Abstract Non-alcoholic fatty liver disease (NAFLD) is currently a growing epidemic disease that can lead to cirrhosis and hepatic cancer when it evolves into non-alcoholic steatohepatitis (NASH), a gap not well understood. To characterize this disease, pigs, considered to be one of the most similar to human experimental animal models, were used. To date, all swine-based settings have been carried out using rare predisposed breeds or long-term experiments. Herein, we fully describe a new experimental swine model for initial and reversible NASH using cross-bred animals fed on a high saturated fat, fructose, cholesterol, cholate, choline and methionine-deficient diet. To gain insight into the hepatic transcriptome that undergoes steatosis and steatohepatitis, we used RNA sequencing. This process significantly up-regulated 976 and down-regulated 209 genes mainly involved in cellular processes. Gene expression changes of 22 selected transcripts were verified by RT-qPCR. Lipid droplet area was positively associated with CD68, GPNMB, LGALS3, SLC51B and SPP1, and negatively with SQLE expressions. When these genes were tested in a second experiment of NASH reversion, LGALS3, SLC51B and SPP1 significantly decreased their expression. However, only LGALS3 was associated with lipid droplet areas. Our results suggest a role for LGALS3 in the transition of NAFLD to NASH. |
| first_indexed | 2024-04-11T18:15:10Z |
| format | Article |
| id | doaj.art-5bc1f80a5eda47a8860a5cd2f123e276 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T18:15:10Z |
| publishDate | 2022-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-5bc1f80a5eda47a8860a5cd2f123e2762022-12-22T04:09:54ZengNature PortfolioScientific Reports2045-23222022-01-0112111710.1038/s41598-022-04971-zHepatic galectin-3 is associated with lipid droplet area in non-alcoholic steatohepatitis in a new swine modelLuis V. Herrera-Marcos0Roberto Martínez-Beamonte1Manuel Macías-Herranz2Carmen Arnal3Cristina Barranquero4Juan J. Puente-Lanzarote5Sonia Gascón6Tania Herrero-Continente7Gonzalo Gonzalo-Romeo8Víctor Alastrué-Vera9Dolores Gutiérrez-Blázquez10José M. Lou-Bonafonte11Joaquín C. Surra12María J. Rodríguez-Yoldi13Agustín García-Gil14Antonio Güemes15Jesús Osada16Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Instituto de Investigación Sanitaria de Aragón-Universidad de ZaragozaDepartamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Instituto de Investigación Sanitaria de Aragón-Universidad de ZaragozaDepartamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Instituto de Investigación Sanitaria de Aragón-Universidad de ZaragozaInstituto Agroalimentario de Aragón, CITA-Universidad de ZaragozaDepartamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Instituto de Investigación Sanitaria de Aragón-Universidad de ZaragozaServicio de Bioquímica Clínica. Hospital, Clínico Universitario Lozano BlesaDepartamento de Farmacología, Fisiología, Medicina Legal y Forense, Instituto de Investigación Sanitaria de Aragón-Universidad de ZaragozaDepartamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Instituto de Investigación Sanitaria de Aragón-Universidad de ZaragozaServicio General de Apoyo a la Investigación. División de Experimentación Animal, Universidad de ZaragozaEbers Medical TechnologyUnidad de Proteómica, Universidad Complutense de MadridInstituto Agroalimentario de Aragón, CITA-Universidad de ZaragozaInstituto Agroalimentario de Aragón, CITA-Universidad de ZaragozaInstituto Agroalimentario de Aragón, CITA-Universidad de ZaragozaDepartamento de Cirugía, Facultad de Medicina, Instituto de Investigación Sanitaria de Aragón-Universidad de ZaragozaDepartamento de Cirugía, Facultad de Medicina, Instituto de Investigación Sanitaria de Aragón-Universidad de ZaragozaDepartamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Instituto de Investigación Sanitaria de Aragón-Universidad de ZaragozaAbstract Non-alcoholic fatty liver disease (NAFLD) is currently a growing epidemic disease that can lead to cirrhosis and hepatic cancer when it evolves into non-alcoholic steatohepatitis (NASH), a gap not well understood. To characterize this disease, pigs, considered to be one of the most similar to human experimental animal models, were used. To date, all swine-based settings have been carried out using rare predisposed breeds or long-term experiments. Herein, we fully describe a new experimental swine model for initial and reversible NASH using cross-bred animals fed on a high saturated fat, fructose, cholesterol, cholate, choline and methionine-deficient diet. To gain insight into the hepatic transcriptome that undergoes steatosis and steatohepatitis, we used RNA sequencing. This process significantly up-regulated 976 and down-regulated 209 genes mainly involved in cellular processes. Gene expression changes of 22 selected transcripts were verified by RT-qPCR. Lipid droplet area was positively associated with CD68, GPNMB, LGALS3, SLC51B and SPP1, and negatively with SQLE expressions. When these genes were tested in a second experiment of NASH reversion, LGALS3, SLC51B and SPP1 significantly decreased their expression. However, only LGALS3 was associated with lipid droplet areas. Our results suggest a role for LGALS3 in the transition of NAFLD to NASH.https://doi.org/10.1038/s41598-022-04971-z |
| spellingShingle | Luis V. Herrera-Marcos Roberto Martínez-Beamonte Manuel Macías-Herranz Carmen Arnal Cristina Barranquero Juan J. Puente-Lanzarote Sonia Gascón Tania Herrero-Continente Gonzalo Gonzalo-Romeo Víctor Alastrué-Vera Dolores Gutiérrez-Blázquez José M. Lou-Bonafonte Joaquín C. Surra María J. Rodríguez-Yoldi Agustín García-Gil Antonio Güemes Jesús Osada Hepatic galectin-3 is associated with lipid droplet area in non-alcoholic steatohepatitis in a new swine model Scientific Reports |
| title | Hepatic galectin-3 is associated with lipid droplet area in non-alcoholic steatohepatitis in a new swine model |
| title_full | Hepatic galectin-3 is associated with lipid droplet area in non-alcoholic steatohepatitis in a new swine model |
| title_fullStr | Hepatic galectin-3 is associated with lipid droplet area in non-alcoholic steatohepatitis in a new swine model |
| title_full_unstemmed | Hepatic galectin-3 is associated with lipid droplet area in non-alcoholic steatohepatitis in a new swine model |
| title_short | Hepatic galectin-3 is associated with lipid droplet area in non-alcoholic steatohepatitis in a new swine model |
| title_sort | hepatic galectin 3 is associated with lipid droplet area in non alcoholic steatohepatitis in a new swine model |
| url | https://doi.org/10.1038/s41598-022-04971-z |
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