Protocol for in vitro analysis of pro-inflammatory and metabolic functions of cultured primary murine astrocytes

Protocol for in vitro analysis of pro-inflammatory and metabolic functions of cultured primary murine astrocytes

Summary: Robust protocols are required to investigate in vitro the molecular mechanisms that control astrocyte metabolism and pro-inflammatory activities. In the present protocol, we describe step by step the isolation and culture of primary murine astrocytes from neonatal brains, followed by their...

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Bibliographic Details
Main Authors: Cristina Gutiérrez-Vázquez, Francisco J. Quintana
Format: Article
Language:English
Published: Elsevier 2022-03-01
Series:STAR Protocols
Subjects:
Cell culture
Cell isolation
Immunology
Metabolism
Neuroscience
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721007395

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