Functional Analysis of <i>SmMYB39</i> in Salt Stress Tolerance of Eggplant (<i>Solanum melongena</i> L.)
Eggplant (<i>Solanum melongena</i> L.), a widely cultivated vegetable of the Solanaceae family, faces significant challenges in growth and yield due to soil salinization. This study aimed to investigate the functional role of the transcription factor <i>SmMYB39</i> in salt st...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/8/848 |
| _version_ | 1827729632884948992 |
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| author | Zheng Jiang Lei Shen Jie He Lihui Du Xin Xia Longhao Zhang Xu Yang |
| author_facet | Zheng Jiang Lei Shen Jie He Lihui Du Xin Xia Longhao Zhang Xu Yang |
| author_sort | Zheng Jiang |
| collection | DOAJ |
| description | Eggplant (<i>Solanum melongena</i> L.), a widely cultivated vegetable of the Solanaceae family, faces significant challenges in growth and yield due to soil salinization. This study aimed to investigate the functional role of the transcription factor <i>SmMYB39</i> in salt stress tolerance in eggplant. This investigation was conducted through the utilization of bioinformatics analysis, quantitative real-time polymerase chain reaction (qRT-PCR), subcellular localization, validation of transcriptional activation activity, Virus-Induced Gene Silencing (VIGS), and protein interactome analysis. Bioinformatics analysis revealed that <i>SmMYB39</i> has the closest relationship with <i>SlMYB41</i>, and its promoter contains multiple stress-responsive elements. qRT-PCR results demonstrated that <i>SmMYB39</i> was significantly upregulated after 12 h of salt stress. Subcellular localization results indicated that the SmMYB39 protein is localized in the nucleus and exhibits transcriptional activation activity. Using VIGS, we observed that silencing of <i>SmMYB39</i> led to reduced salt stress tolerance in eggplant. In addition, we have conducted research on the protein interactome of SmMYB39. In conclusion, our study demonstrates that <i>SmMYB39</i> is a crucial transcription factor involved in salt stress response and has the potential to enhance salt tolerance in eggplant. |
| first_indexed | 2024-03-10T23:54:19Z |
| format | Article |
| id | doaj.art-5bc3a6eb4dd6424c91e342f144c3735b |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T23:54:19Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-5bc3a6eb4dd6424c91e342f144c3735b2023-11-19T01:20:35ZengMDPI AGHorticulturae2311-75242023-07-019884810.3390/horticulturae9080848Functional Analysis of <i>SmMYB39</i> in Salt Stress Tolerance of Eggplant (<i>Solanum melongena</i> L.)Zheng Jiang0Lei Shen1Jie He2Lihui Du3Xin Xia4Longhao Zhang5Xu Yang6College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaEggplant (<i>Solanum melongena</i> L.), a widely cultivated vegetable of the Solanaceae family, faces significant challenges in growth and yield due to soil salinization. This study aimed to investigate the functional role of the transcription factor <i>SmMYB39</i> in salt stress tolerance in eggplant. This investigation was conducted through the utilization of bioinformatics analysis, quantitative real-time polymerase chain reaction (qRT-PCR), subcellular localization, validation of transcriptional activation activity, Virus-Induced Gene Silencing (VIGS), and protein interactome analysis. Bioinformatics analysis revealed that <i>SmMYB39</i> has the closest relationship with <i>SlMYB41</i>, and its promoter contains multiple stress-responsive elements. qRT-PCR results demonstrated that <i>SmMYB39</i> was significantly upregulated after 12 h of salt stress. Subcellular localization results indicated that the SmMYB39 protein is localized in the nucleus and exhibits transcriptional activation activity. Using VIGS, we observed that silencing of <i>SmMYB39</i> led to reduced salt stress tolerance in eggplant. In addition, we have conducted research on the protein interactome of SmMYB39. In conclusion, our study demonstrates that <i>SmMYB39</i> is a crucial transcription factor involved in salt stress response and has the potential to enhance salt tolerance in eggplant.https://www.mdpi.com/2311-7524/9/8/848eggplantsalt stressMYB transcription factorfunctional analysisVIGS |
| spellingShingle | Zheng Jiang Lei Shen Jie He Lihui Du Xin Xia Longhao Zhang Xu Yang Functional Analysis of <i>SmMYB39</i> in Salt Stress Tolerance of Eggplant (<i>Solanum melongena</i> L.) Horticulturae eggplant salt stress MYB transcription factor functional analysis VIGS |
| title | Functional Analysis of <i>SmMYB39</i> in Salt Stress Tolerance of Eggplant (<i>Solanum melongena</i> L.) |
| title_full | Functional Analysis of <i>SmMYB39</i> in Salt Stress Tolerance of Eggplant (<i>Solanum melongena</i> L.) |
| title_fullStr | Functional Analysis of <i>SmMYB39</i> in Salt Stress Tolerance of Eggplant (<i>Solanum melongena</i> L.) |
| title_full_unstemmed | Functional Analysis of <i>SmMYB39</i> in Salt Stress Tolerance of Eggplant (<i>Solanum melongena</i> L.) |
| title_short | Functional Analysis of <i>SmMYB39</i> in Salt Stress Tolerance of Eggplant (<i>Solanum melongena</i> L.) |
| title_sort | functional analysis of i smmyb39 i in salt stress tolerance of eggplant i solanum melongena i l |
| topic | eggplant salt stress MYB transcription factor functional analysis VIGS |
| url | https://www.mdpi.com/2311-7524/9/8/848 |
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