| Summary: | <i>Bacillus subtilis</i> is a gram-positive bacterium, a promising microorganism due to its strong extracellular protein secretion ability, non-toxic, and relatively mature industrial fermentation technology. However, cell autolysis during fermentation restricts the industrial application of <i>B. subtilis</i>. With the fast advancement of molecular biology and genetic engineering technology, various advanced procedures and gene editing tools have been used to successfully construct autolysis-resistant <i>B. subtilis</i> chassis cells to manufacture various biological products. This paper first analyses the causes of autolysis in <i>B. subtilis</i> from a mechanistic perspective and outlines various strategies to address autolysis in <i>B. subtilis</i>. Finally, potential strategies for solving the autolysis problem of <i>B. subtilis</i> are foreseen.
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