Functionalization of OMVs for Biocatalytic Applications
Outer membrane vesicles (OMVs) are miniature versions of gram-negative bacteria that contain almost the same content as their parent cells, particularly in terms of membrane composition. Using OMVs as biocatalysts is a promising approach due to their potential benefits, including their ability to be...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Membranes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-0375/13/5/459 |
| _version_ | 1797599126148349952 |
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| author | Lita Amalia Shen-Long Tsai |
| author_facet | Lita Amalia Shen-Long Tsai |
| author_sort | Lita Amalia |
| collection | DOAJ |
| description | Outer membrane vesicles (OMVs) are miniature versions of gram-negative bacteria that contain almost the same content as their parent cells, particularly in terms of membrane composition. Using OMVs as biocatalysts is a promising approach due to their potential benefits, including their ability to be handled similarly to bacteria while lacking potentially pathogenic organisms. To employ OMVs as biocatalysts, they must be functionalized with immobilized enzymes to the OMV platform. Various enzyme immobilization techniques are available, including surface display and encapsulation, each with advantages and disadvantages depending on the objectives. This review provides a concise yet comprehensive overview of these immobilization techniques and their applications in utilizing OMVs as biocatalysts. Specifically, we discuss the use of OMVs in catalyzing the conversion of chemical compounds, their role in polymer degradation, and their performance in bioremediation. |
| first_indexed | 2024-03-11T03:30:18Z |
| format | Article |
| id | doaj.art-5bc5913ea77d428ea86eff06f769ac33 |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-11T03:30:18Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-5bc5913ea77d428ea86eff06f769ac332023-11-18T02:23:40ZengMDPI AGMembranes2077-03752023-04-0113545910.3390/membranes13050459Functionalization of OMVs for Biocatalytic ApplicationsLita Amalia0Shen-Long Tsai1Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei City 10607, TaiwanDepartment of Chemical Engineering, National Taiwan University of Science and Technology, Taipei City 10607, TaiwanOuter membrane vesicles (OMVs) are miniature versions of gram-negative bacteria that contain almost the same content as their parent cells, particularly in terms of membrane composition. Using OMVs as biocatalysts is a promising approach due to their potential benefits, including their ability to be handled similarly to bacteria while lacking potentially pathogenic organisms. To employ OMVs as biocatalysts, they must be functionalized with immobilized enzymes to the OMV platform. Various enzyme immobilization techniques are available, including surface display and encapsulation, each with advantages and disadvantages depending on the objectives. This review provides a concise yet comprehensive overview of these immobilization techniques and their applications in utilizing OMVs as biocatalysts. Specifically, we discuss the use of OMVs in catalyzing the conversion of chemical compounds, their role in polymer degradation, and their performance in bioremediation.https://www.mdpi.com/2077-0375/13/5/459vesiclefunctionalizationimmobilizationenzymecatalyst |
| spellingShingle | Lita Amalia Shen-Long Tsai Functionalization of OMVs for Biocatalytic Applications Membranes vesicle functionalization immobilization enzyme catalyst |
| title | Functionalization of OMVs for Biocatalytic Applications |
| title_full | Functionalization of OMVs for Biocatalytic Applications |
| title_fullStr | Functionalization of OMVs for Biocatalytic Applications |
| title_full_unstemmed | Functionalization of OMVs for Biocatalytic Applications |
| title_short | Functionalization of OMVs for Biocatalytic Applications |
| title_sort | functionalization of omvs for biocatalytic applications |
| topic | vesicle functionalization immobilization enzyme catalyst |
| url | https://www.mdpi.com/2077-0375/13/5/459 |
| work_keys_str_mv | AT litaamalia functionalizationofomvsforbiocatalyticapplications AT shenlongtsai functionalizationofomvsforbiocatalyticapplications |