Advantages of repeated low dose against single high dose of kainate in C57BL/6J mouse model of status epilepticus: behavioral and electroencephalographic studies.
A refined kainate (KA) C57BL/6J mouse model of status epilepticus (SE) using a repeated low dose (RLD) of KA (5 mg/kg, intraperitoneal; at 30 min intervals) was compared with the established single high dose (SHD) of KA (20 mg/kg, intraperitoneal) model. In the RLD group, increased duration of convu...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4011859?pdf=render |
| _version_ | 1818922995693912064 |
|---|---|
| author | Karen Tse Sreekanth Puttachary Edward Beamer Graeme J Sills Thimmasettappa Thippeswamy |
| author_facet | Karen Tse Sreekanth Puttachary Edward Beamer Graeme J Sills Thimmasettappa Thippeswamy |
| author_sort | Karen Tse |
| collection | DOAJ |
| description | A refined kainate (KA) C57BL/6J mouse model of status epilepticus (SE) using a repeated low dose (RLD) of KA (5 mg/kg, intraperitoneal; at 30 min intervals) was compared with the established single high dose (SHD) of KA (20 mg/kg, intraperitoneal) model. In the RLD group, increased duration of convulsive motor seizures (CMS, Racine scale stage ≥3) with a significant reduction in mortality from 21% to 6% and decreased variability in seizure severity between animals/batches were observed when compared to the SHD group. There was a significant increase in the percentage of animals that reached stage-5 seizures (65% versus 96%) in the RLD group. Integrated real-time video-EEG analysis of both groups, using NeuroScore software, revealed stage-specific spikes and power spectral density characteristics. When the seizures progressed from non-convulsive seizures (NCS, stage 1-2) to CMS (stage 3-5), the delta power decreased which was followed by an increase in gamma and beta power. A transient increase in alpha and sigma power marked the transition from NCS to CMS with characteristic 'high frequency trigger' spikes on the EEG, which had no behavioral expression. During SE the spike rate was higher in the RLD group than in the SHD group. Overall these results confirm that RLD of KA is a more robust and consistent mouse model of SE than the SHD of KA mouse model. |
| first_indexed | 2024-12-20T02:02:24Z |
| format | Article |
| id | doaj.art-5bc93a1e2d3b455dad32b0837be33cec |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T02:02:24Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5bc93a1e2d3b455dad32b0837be33cec2022-12-21T19:57:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0195e9662210.1371/journal.pone.0096622Advantages of repeated low dose against single high dose of kainate in C57BL/6J mouse model of status epilepticus: behavioral and electroencephalographic studies.Karen TseSreekanth PuttacharyEdward BeamerGraeme J SillsThimmasettappa ThippeswamyA refined kainate (KA) C57BL/6J mouse model of status epilepticus (SE) using a repeated low dose (RLD) of KA (5 mg/kg, intraperitoneal; at 30 min intervals) was compared with the established single high dose (SHD) of KA (20 mg/kg, intraperitoneal) model. In the RLD group, increased duration of convulsive motor seizures (CMS, Racine scale stage ≥3) with a significant reduction in mortality from 21% to 6% and decreased variability in seizure severity between animals/batches were observed when compared to the SHD group. There was a significant increase in the percentage of animals that reached stage-5 seizures (65% versus 96%) in the RLD group. Integrated real-time video-EEG analysis of both groups, using NeuroScore software, revealed stage-specific spikes and power spectral density characteristics. When the seizures progressed from non-convulsive seizures (NCS, stage 1-2) to CMS (stage 3-5), the delta power decreased which was followed by an increase in gamma and beta power. A transient increase in alpha and sigma power marked the transition from NCS to CMS with characteristic 'high frequency trigger' spikes on the EEG, which had no behavioral expression. During SE the spike rate was higher in the RLD group than in the SHD group. Overall these results confirm that RLD of KA is a more robust and consistent mouse model of SE than the SHD of KA mouse model.http://europepmc.org/articles/PMC4011859?pdf=render |
| spellingShingle | Karen Tse Sreekanth Puttachary Edward Beamer Graeme J Sills Thimmasettappa Thippeswamy Advantages of repeated low dose against single high dose of kainate in C57BL/6J mouse model of status epilepticus: behavioral and electroencephalographic studies. PLoS ONE |
| title | Advantages of repeated low dose against single high dose of kainate in C57BL/6J mouse model of status epilepticus: behavioral and electroencephalographic studies. |
| title_full | Advantages of repeated low dose against single high dose of kainate in C57BL/6J mouse model of status epilepticus: behavioral and electroencephalographic studies. |
| title_fullStr | Advantages of repeated low dose against single high dose of kainate in C57BL/6J mouse model of status epilepticus: behavioral and electroencephalographic studies. |
| title_full_unstemmed | Advantages of repeated low dose against single high dose of kainate in C57BL/6J mouse model of status epilepticus: behavioral and electroencephalographic studies. |
| title_short | Advantages of repeated low dose against single high dose of kainate in C57BL/6J mouse model of status epilepticus: behavioral and electroencephalographic studies. |
| title_sort | advantages of repeated low dose against single high dose of kainate in c57bl 6j mouse model of status epilepticus behavioral and electroencephalographic studies |
| url | http://europepmc.org/articles/PMC4011859?pdf=render |
| work_keys_str_mv | AT karentse advantagesofrepeatedlowdoseagainstsinglehighdoseofkainateinc57bl6jmousemodelofstatusepilepticusbehavioralandelectroencephalographicstudies AT sreekanthputtachary advantagesofrepeatedlowdoseagainstsinglehighdoseofkainateinc57bl6jmousemodelofstatusepilepticusbehavioralandelectroencephalographicstudies AT edwardbeamer advantagesofrepeatedlowdoseagainstsinglehighdoseofkainateinc57bl6jmousemodelofstatusepilepticusbehavioralandelectroencephalographicstudies AT graemejsills advantagesofrepeatedlowdoseagainstsinglehighdoseofkainateinc57bl6jmousemodelofstatusepilepticusbehavioralandelectroencephalographicstudies AT thimmasettappathippeswamy advantagesofrepeatedlowdoseagainstsinglehighdoseofkainateinc57bl6jmousemodelofstatusepilepticusbehavioralandelectroencephalographicstudies |