Bacterial and Fungal Community Structures in Loess Plateau Grasslands with Different Grazing Intensities
The Loess Plateau of China is one of the most fragile ecosystems worldwide; thus, human production activities need to be conducted very cautiously. In this study, MiSeq high-throughput sequencing was applied to assess the relationship between bacterial and fungal community structures and changes in...
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Frontiers Media S.A.
2017-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.00606/full |
| _version_ | 1818042567866974208 |
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| author | Huhe Huhe Xianjiang Chen Fujiang Hou Yanpei Wu Yunxiang Cheng |
| author_facet | Huhe Huhe Xianjiang Chen Fujiang Hou Yanpei Wu Yunxiang Cheng |
| author_sort | Huhe |
| collection | DOAJ |
| description | The Loess Plateau of China is one of the most fragile ecosystems worldwide; thus, human production activities need to be conducted very cautiously. In this study, MiSeq high-throughput sequencing was applied to assess the relationship between bacterial and fungal community structures and changes in vegetation and soil physical and chemical properties induced by grazing, in four grasslands with different levels of grazing intensity (0, 2.67, 5.33, and 8.67 sheep/ha) in the semiarid region of the Loess Plateau. The relative abundances of the bacterial community in the grasslands with 2.67 and 5.33 sheep/ha were significantly higher than those in grasslands with 0 and 8.67 sheep/ha, and the fungal diversity was significantly lower for grasslands with 2.67 sheep/ha than for the other grasslands. Redundancy analysis (RDA) showed that plant biomass, nitrate, and total nitrogen have significant effects on bacterial community structure, whereas nitrate and total nitrogen also significantly affect fungal community structure. Variation partitioning showed that soil and plant characteristics influence the bacterial and fungal community structures; these characteristics explained 51.9 and 52.9% of the variation, respectively. Thus, bacterial and fungal community structures are very sensitive to grazing activity and change to different extents with different grazing intensities. Based on our findings, a grazing intensity of about 2.67 sheep/ha is considered the most appropriate in semiarid grassland of the Loess Plateau. |
| first_indexed | 2024-12-10T08:48:23Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T08:48:23Z |
| publishDate | 2017-04-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-5bcb5949870c46ef9e89fd003d1d6fae2022-12-22T01:55:41ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-04-01810.3389/fmicb.2017.00606241748Bacterial and Fungal Community Structures in Loess Plateau Grasslands with Different Grazing Intensities Huhe0 Huhe1Xianjiang Chen2Fujiang Hou3Yanpei Wu4Yunxiang Cheng5Soil Fertilizer and Water-Saving Institute, Gansu Academy of Agricultural SciencesLanzhou, ChinaThe Ministry of Agriculture in Gansu Province Cultivated Land Conservation and Agricultural Environmental Science Observation Experiment StationsWuwei, ChinaState Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou UniversityLanzhou, ChinaState Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou UniversityLanzhou, ChinaState Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou UniversityLanzhou, ChinaState Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou UniversityLanzhou, ChinaThe Loess Plateau of China is one of the most fragile ecosystems worldwide; thus, human production activities need to be conducted very cautiously. In this study, MiSeq high-throughput sequencing was applied to assess the relationship between bacterial and fungal community structures and changes in vegetation and soil physical and chemical properties induced by grazing, in four grasslands with different levels of grazing intensity (0, 2.67, 5.33, and 8.67 sheep/ha) in the semiarid region of the Loess Plateau. The relative abundances of the bacterial community in the grasslands with 2.67 and 5.33 sheep/ha were significantly higher than those in grasslands with 0 and 8.67 sheep/ha, and the fungal diversity was significantly lower for grasslands with 2.67 sheep/ha than for the other grasslands. Redundancy analysis (RDA) showed that plant biomass, nitrate, and total nitrogen have significant effects on bacterial community structure, whereas nitrate and total nitrogen also significantly affect fungal community structure. Variation partitioning showed that soil and plant characteristics influence the bacterial and fungal community structures; these characteristics explained 51.9 and 52.9% of the variation, respectively. Thus, bacterial and fungal community structures are very sensitive to grazing activity and change to different extents with different grazing intensities. Based on our findings, a grazing intensity of about 2.67 sheep/ha is considered the most appropriate in semiarid grassland of the Loess Plateau.http://journal.frontiersin.org/article/10.3389/fmicb.2017.00606/fullLoess Plateau grasslandgrazing intensitybacterial communityfungal communityIllumina MiSeq platform |
| spellingShingle | Huhe Huhe Xianjiang Chen Fujiang Hou Yanpei Wu Yunxiang Cheng Bacterial and Fungal Community Structures in Loess Plateau Grasslands with Different Grazing Intensities Frontiers in Microbiology Loess Plateau grassland grazing intensity bacterial community fungal community Illumina MiSeq platform |
| title | Bacterial and Fungal Community Structures in Loess Plateau Grasslands with Different Grazing Intensities |
| title_full | Bacterial and Fungal Community Structures in Loess Plateau Grasslands with Different Grazing Intensities |
| title_fullStr | Bacterial and Fungal Community Structures in Loess Plateau Grasslands with Different Grazing Intensities |
| title_full_unstemmed | Bacterial and Fungal Community Structures in Loess Plateau Grasslands with Different Grazing Intensities |
| title_short | Bacterial and Fungal Community Structures in Loess Plateau Grasslands with Different Grazing Intensities |
| title_sort | bacterial and fungal community structures in loess plateau grasslands with different grazing intensities |
| topic | Loess Plateau grassland grazing intensity bacterial community fungal community Illumina MiSeq platform |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.00606/full |
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