A Direct Immunoassay Based on Surface-Enhanced Spectroscopy Using AuNP/PS-b-P2VP Nanocomposites
A biosensor was developed for directly detecting human immunoglobulin G (IgG) and adenosine triphosphate (ATP) based on stable and reproducible gold nanoparticles/polystyrene-b-poly(2-vinylpyridine) (AuNP/PS-b-P2VP) nanocomposites. The substrates were functionalized with carboxylic acid groups for t...
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MDPI AG
2023-05-01
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| Online Access: | https://www.mdpi.com/1424-8220/23/10/4810 |
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| author | Moyra F. Vieira Ana Lívia de Carvalho Bovolato Bruno G. da Fonseca Celly M. S. Izumi Alexandre G. Brolo |
| author_facet | Moyra F. Vieira Ana Lívia de Carvalho Bovolato Bruno G. da Fonseca Celly M. S. Izumi Alexandre G. Brolo |
| author_sort | Moyra F. Vieira |
| collection | DOAJ |
| description | A biosensor was developed for directly detecting human immunoglobulin G (IgG) and adenosine triphosphate (ATP) based on stable and reproducible gold nanoparticles/polystyrene-b-poly(2-vinylpyridine) (AuNP/PS-b-P2VP) nanocomposites. The substrates were functionalized with carboxylic acid groups for the covalent binding of anti-IgG and anti-ATP and the detection of IgG and ATP (1 to 150 μg/mL). SEM images of the nanocomposite show 17 ± 2 nm AuNP clusters adsorbed over a continuous porous PS-b-P2VP thin film. UV–VIS and SERS were used to characterize each step of the substrate functionalization and the specific interaction between anti-IgG and the targeted IgG analyte. The UV–VIS results show a redshift of the LSPR band as the AuNP surface was functionalized and SERS measurements showed consistent changes in the spectral features. Principal component analysis (PCA) was used to discriminate between samples before and after the affinity tests. Moreover, the designed biosensor proved to be sensitive to different concentrations of IgG with a limit-of-detection (LOD) down to 1 μg/mL. Moreover, the selectivity to IgG was confirmed using standard solutions of IgM as a control. Finally, ATP direct immunoassay (LOD = 1 μg/mL) has demonstrated that this nanocomposite platform can be used to detect different types of biomolecules after proper functionalization. |
| first_indexed | 2024-03-11T03:20:19Z |
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| id | doaj.art-5bccd60a296c4998aa71608a81ff63d9 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-11T03:20:19Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-5bccd60a296c4998aa71608a81ff63d92023-11-18T03:12:54ZengMDPI AGSensors1424-82202023-05-012310481010.3390/s23104810A Direct Immunoassay Based on Surface-Enhanced Spectroscopy Using AuNP/PS-b-P2VP NanocompositesMoyra F. Vieira0Ana Lívia de Carvalho Bovolato1Bruno G. da Fonseca2Celly M. S. Izumi3Alexandre G. Brolo4Department of Chemistry and Center for Advanced Materials and Related Technologies, University of Victoria, P.O. Box 3065, Victoria, BC V8W 3V6, CanadaDepartment of Chemistry and Center for Advanced Materials and Related Technologies, University of Victoria, P.O. Box 3065, Victoria, BC V8W 3V6, CanadaDepartment of Chemistry and Center for Advanced Materials and Related Technologies, University of Victoria, P.O. Box 3065, Victoria, BC V8W 3V6, CanadaDepartamento de Química, Instituto de Ciências Exatas, Universidade Federal de Juiz de Fora, Campus Universitário s/n, CEP, Juiz de Fora 36036-900, BrazilDepartment of Chemistry and Center for Advanced Materials and Related Technologies, University of Victoria, P.O. Box 3065, Victoria, BC V8W 3V6, CanadaA biosensor was developed for directly detecting human immunoglobulin G (IgG) and adenosine triphosphate (ATP) based on stable and reproducible gold nanoparticles/polystyrene-b-poly(2-vinylpyridine) (AuNP/PS-b-P2VP) nanocomposites. The substrates were functionalized with carboxylic acid groups for the covalent binding of anti-IgG and anti-ATP and the detection of IgG and ATP (1 to 150 μg/mL). SEM images of the nanocomposite show 17 ± 2 nm AuNP clusters adsorbed over a continuous porous PS-b-P2VP thin film. UV–VIS and SERS were used to characterize each step of the substrate functionalization and the specific interaction between anti-IgG and the targeted IgG analyte. The UV–VIS results show a redshift of the LSPR band as the AuNP surface was functionalized and SERS measurements showed consistent changes in the spectral features. Principal component analysis (PCA) was used to discriminate between samples before and after the affinity tests. Moreover, the designed biosensor proved to be sensitive to different concentrations of IgG with a limit-of-detection (LOD) down to 1 μg/mL. Moreover, the selectivity to IgG was confirmed using standard solutions of IgM as a control. Finally, ATP direct immunoassay (LOD = 1 μg/mL) has demonstrated that this nanocomposite platform can be used to detect different types of biomolecules after proper functionalization.https://www.mdpi.com/1424-8220/23/10/4810polystyrene-b-poly(2-vinylpyridine)gold nanoparticlesLSPR sensorSERS sensingdirect immunoassay |
| spellingShingle | Moyra F. Vieira Ana Lívia de Carvalho Bovolato Bruno G. da Fonseca Celly M. S. Izumi Alexandre G. Brolo A Direct Immunoassay Based on Surface-Enhanced Spectroscopy Using AuNP/PS-b-P2VP Nanocomposites Sensors polystyrene-b-poly(2-vinylpyridine) gold nanoparticles LSPR sensor SERS sensing direct immunoassay |
| title | A Direct Immunoassay Based on Surface-Enhanced Spectroscopy Using AuNP/PS-b-P2VP Nanocomposites |
| title_full | A Direct Immunoassay Based on Surface-Enhanced Spectroscopy Using AuNP/PS-b-P2VP Nanocomposites |
| title_fullStr | A Direct Immunoassay Based on Surface-Enhanced Spectroscopy Using AuNP/PS-b-P2VP Nanocomposites |
| title_full_unstemmed | A Direct Immunoassay Based on Surface-Enhanced Spectroscopy Using AuNP/PS-b-P2VP Nanocomposites |
| title_short | A Direct Immunoassay Based on Surface-Enhanced Spectroscopy Using AuNP/PS-b-P2VP Nanocomposites |
| title_sort | direct immunoassay based on surface enhanced spectroscopy using aunp ps b p2vp nanocomposites |
| topic | polystyrene-b-poly(2-vinylpyridine) gold nanoparticles LSPR sensor SERS sensing direct immunoassay |
| url | https://www.mdpi.com/1424-8220/23/10/4810 |
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