Expression of Pro-Angiogenic Markers Is Enhanced by Blue Light in Human RPE Cells
Inherited retinal dystrophies are characterized by photoreceptor death. Oxidative stress usually occurs, increasing vision loss, and oxidative damage is often reported in retinitis pigmentosa (RP). More than 300 genes have been reported as RP causing. In contrast, choroidal neovascularization (CNV)...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-11-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/9/11/1154 |
| _version_ | 1797547334991609856 |
|---|---|
| author | Concetta Scimone Simona Alibrandi Sergio Zaccaria Scalinci Edoardo Trovato Battagliola Rosalia D’Angelo Antonina Sidoti Luigi Donato |
| author_facet | Concetta Scimone Simona Alibrandi Sergio Zaccaria Scalinci Edoardo Trovato Battagliola Rosalia D’Angelo Antonina Sidoti Luigi Donato |
| author_sort | Concetta Scimone |
| collection | DOAJ |
| description | Inherited retinal dystrophies are characterized by photoreceptor death. Oxidative stress usually occurs, increasing vision loss, and oxidative damage is often reported in retinitis pigmentosa (RP). More than 300 genes have been reported as RP causing. In contrast, choroidal neovascularization (CNV) only occasionally develops in the late stages of RP. We herein study the regulation of RP causative genes that are likely linked to CNV onset under oxidative conditions. We studied how the endogenous adduct <i>N</i>-retinylidene-<i>N</i>-retinylethanolamine (A2E) affects the expression of angiogenic markers in human retinal pigment epithelium (H-RPE) cells and a possible correlation with RP-causing genes. H-RPE cells were exposed to A2E and blue light for 3 and 6h. By transcriptome analysis, genes differentially expressed between A2E-treated cells and untreated ones were detected. The quantification of differential gene expression was performed by the Limma R package. Enrichment pathway analysis by the FunRich tool and gene prioritization by ToppGene allowed us to identify dysregulated genes involved in angiogenesis and linked to RP development. Two RP causative genes, <i>AHR</i> and <i>ROM1</i>, can be associated with an increased risk of CNV development. Genetic analysis of RP patients affected by CNV will confirm this hypothesis. |
| first_indexed | 2024-03-10T14:42:49Z |
| format | Article |
| id | doaj.art-5bccf9d988fd45a393e8d9f1464e5cec |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T14:42:49Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-5bccf9d988fd45a393e8d9f1464e5cec2023-11-20T21:39:30ZengMDPI AGAntioxidants2076-39212020-11-01911115410.3390/antiox9111154Expression of Pro-Angiogenic Markers Is Enhanced by Blue Light in Human RPE CellsConcetta Scimone0Simona Alibrandi1Sergio Zaccaria Scalinci2Edoardo Trovato Battagliola3Rosalia D’Angelo4Antonina Sidoti5Luigi Donato6Department of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, 98125 Messina, ItalyDepartment of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, 98125 Messina, ItalyDIMEC (Department of Medical and Surgical Sciences), University of Bologna, 40121 Bologna, ItalyDIMEC (Department of Medical and Surgical Sciences), University of Bologna, 40121 Bologna, ItalyDepartment of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, 98125 Messina, ItalyDepartment of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, 98125 Messina, ItalyDepartment of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, 98125 Messina, ItalyInherited retinal dystrophies are characterized by photoreceptor death. Oxidative stress usually occurs, increasing vision loss, and oxidative damage is often reported in retinitis pigmentosa (RP). More than 300 genes have been reported as RP causing. In contrast, choroidal neovascularization (CNV) only occasionally develops in the late stages of RP. We herein study the regulation of RP causative genes that are likely linked to CNV onset under oxidative conditions. We studied how the endogenous adduct <i>N</i>-retinylidene-<i>N</i>-retinylethanolamine (A2E) affects the expression of angiogenic markers in human retinal pigment epithelium (H-RPE) cells and a possible correlation with RP-causing genes. H-RPE cells were exposed to A2E and blue light for 3 and 6h. By transcriptome analysis, genes differentially expressed between A2E-treated cells and untreated ones were detected. The quantification of differential gene expression was performed by the Limma R package. Enrichment pathway analysis by the FunRich tool and gene prioritization by ToppGene allowed us to identify dysregulated genes involved in angiogenesis and linked to RP development. Two RP causative genes, <i>AHR</i> and <i>ROM1</i>, can be associated with an increased risk of CNV development. Genetic analysis of RP patients affected by CNV will confirm this hypothesis.https://www.mdpi.com/2076-3921/9/11/1154oxidative stressretinitis pigmentosaangiogenesischoroidal neovascularizationbiomarkers |
| spellingShingle | Concetta Scimone Simona Alibrandi Sergio Zaccaria Scalinci Edoardo Trovato Battagliola Rosalia D’Angelo Antonina Sidoti Luigi Donato Expression of Pro-Angiogenic Markers Is Enhanced by Blue Light in Human RPE Cells Antioxidants oxidative stress retinitis pigmentosa angiogenesis choroidal neovascularization biomarkers |
| title | Expression of Pro-Angiogenic Markers Is Enhanced by Blue Light in Human RPE Cells |
| title_full | Expression of Pro-Angiogenic Markers Is Enhanced by Blue Light in Human RPE Cells |
| title_fullStr | Expression of Pro-Angiogenic Markers Is Enhanced by Blue Light in Human RPE Cells |
| title_full_unstemmed | Expression of Pro-Angiogenic Markers Is Enhanced by Blue Light in Human RPE Cells |
| title_short | Expression of Pro-Angiogenic Markers Is Enhanced by Blue Light in Human RPE Cells |
| title_sort | expression of pro angiogenic markers is enhanced by blue light in human rpe cells |
| topic | oxidative stress retinitis pigmentosa angiogenesis choroidal neovascularization biomarkers |
| url | https://www.mdpi.com/2076-3921/9/11/1154 |
| work_keys_str_mv | AT concettascimone expressionofproangiogenicmarkersisenhancedbybluelightinhumanrpecells AT simonaalibrandi expressionofproangiogenicmarkersisenhancedbybluelightinhumanrpecells AT sergiozaccariascalinci expressionofproangiogenicmarkersisenhancedbybluelightinhumanrpecells AT edoardotrovatobattagliola expressionofproangiogenicmarkersisenhancedbybluelightinhumanrpecells AT rosaliadangelo expressionofproangiogenicmarkersisenhancedbybluelightinhumanrpecells AT antoninasidoti expressionofproangiogenicmarkersisenhancedbybluelightinhumanrpecells AT luigidonato expressionofproangiogenicmarkersisenhancedbybluelightinhumanrpecells |