Partially Protective Immunity Induced by a 20 kDa Protein Secreted by Trichinella spiralis Stichocytes.
Trichinella spiralis infection induces protective immunity against re-infection in animal models. Identification of the antigens eliciting acquired immunity during infection is important for vaccine development against Trichinella infection and immunodiagnosis.The T. spiralis adult cDNA library was...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4545582?pdf=render |
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| author | Kuo Bi Jing Yang Lei Wang Yuan Gu Bin Zhan Xinping Zhu |
| author_facet | Kuo Bi Jing Yang Lei Wang Yuan Gu Bin Zhan Xinping Zhu |
| author_sort | Kuo Bi |
| collection | DOAJ |
| description | Trichinella spiralis infection induces protective immunity against re-infection in animal models. Identification of the antigens eliciting acquired immunity during infection is important for vaccine development against Trichinella infection and immunodiagnosis.The T. spiralis adult cDNA library was immunoscreened with sera from pigs experimentally infected with 20,000 infective T. spiralis larvae. Total 43 positive clones encoding for 28 proteins were identified; one of the immunodominant proteins was 20 kDa Ts-ES-1 secreted by Trichinella stichocytes and existing in the excretory/secretory (ES) products of T. spiralis adult and muscle larval worms. Ts-ES-1 contains 172 amino acids with a typical signal peptide in the first 20 amino acids. The expression of Ts-ES-1 was detected in both the adult and muscle larval stages at the mRNA and protein expression levels. Mice immunized with recombinant Ts-ES-1 (rTs-ES-1) formulated with ISA50v2 adjuvant exhibited a significant worm reduction in both the adult worm (27%) and muscle larvae burden (42.1%) after a challenge with T. spiralis compared to the adjuvant control group (p<0.01). The rTs-ES-1-induced protection was associated with a high level of specific anti-Ts-ES-1 IgG antibodies and a Th1/Th2 mixed immune response.The newly identified rTs-ES-1 is an immunodominant protein secreted by Trichinella stichocytes during natural infection and enables to the induction of partial protective immunity in vaccinated mice against Trichinella infection. Therefore, rTs-ES-1 is a potential candidate for vaccine development against trichinellosis. |
| first_indexed | 2024-12-10T09:56:52Z |
| format | Article |
| id | doaj.art-5bce7921865f4589b4ac60fb18476e82 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T09:56:52Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5bce7921865f4589b4ac60fb18476e822022-12-22T01:53:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01108e013618910.1371/journal.pone.0136189Partially Protective Immunity Induced by a 20 kDa Protein Secreted by Trichinella spiralis Stichocytes.Kuo BiJing YangLei WangYuan GuBin ZhanXinping ZhuTrichinella spiralis infection induces protective immunity against re-infection in animal models. Identification of the antigens eliciting acquired immunity during infection is important for vaccine development against Trichinella infection and immunodiagnosis.The T. spiralis adult cDNA library was immunoscreened with sera from pigs experimentally infected with 20,000 infective T. spiralis larvae. Total 43 positive clones encoding for 28 proteins were identified; one of the immunodominant proteins was 20 kDa Ts-ES-1 secreted by Trichinella stichocytes and existing in the excretory/secretory (ES) products of T. spiralis adult and muscle larval worms. Ts-ES-1 contains 172 amino acids with a typical signal peptide in the first 20 amino acids. The expression of Ts-ES-1 was detected in both the adult and muscle larval stages at the mRNA and protein expression levels. Mice immunized with recombinant Ts-ES-1 (rTs-ES-1) formulated with ISA50v2 adjuvant exhibited a significant worm reduction in both the adult worm (27%) and muscle larvae burden (42.1%) after a challenge with T. spiralis compared to the adjuvant control group (p<0.01). The rTs-ES-1-induced protection was associated with a high level of specific anti-Ts-ES-1 IgG antibodies and a Th1/Th2 mixed immune response.The newly identified rTs-ES-1 is an immunodominant protein secreted by Trichinella stichocytes during natural infection and enables to the induction of partial protective immunity in vaccinated mice against Trichinella infection. Therefore, rTs-ES-1 is a potential candidate for vaccine development against trichinellosis.http://europepmc.org/articles/PMC4545582?pdf=render |
| spellingShingle | Kuo Bi Jing Yang Lei Wang Yuan Gu Bin Zhan Xinping Zhu Partially Protective Immunity Induced by a 20 kDa Protein Secreted by Trichinella spiralis Stichocytes. PLoS ONE |
| title | Partially Protective Immunity Induced by a 20 kDa Protein Secreted by Trichinella spiralis Stichocytes. |
| title_full | Partially Protective Immunity Induced by a 20 kDa Protein Secreted by Trichinella spiralis Stichocytes. |
| title_fullStr | Partially Protective Immunity Induced by a 20 kDa Protein Secreted by Trichinella spiralis Stichocytes. |
| title_full_unstemmed | Partially Protective Immunity Induced by a 20 kDa Protein Secreted by Trichinella spiralis Stichocytes. |
| title_short | Partially Protective Immunity Induced by a 20 kDa Protein Secreted by Trichinella spiralis Stichocytes. |
| title_sort | partially protective immunity induced by a 20 kda protein secreted by trichinella spiralis stichocytes |
| url | http://europepmc.org/articles/PMC4545582?pdf=render |
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