Dual Emerging Applications of Solid-State Fermentation (SSF) with <em>Aspergillus niger</em> and Ultrasonic-Assisted Extraction (UAE) for the Obtention of Antimicrobial Polyphenols from Pineapple Waste
The exploration of natural antimicrobial compounds is necessary due to the current bacterial resistance to synthetic antibiotics. For this reason, pineapple residues were evaluated as a natural source of phenolic compounds with antimicrobial capacity. Pineapple residues were fermented with <i>...
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MDPI AG
2023-07-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/8/706 |
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| author | Sarah L. Paz-Arteaga Edith Cadena-Chamorro Liliana Serna-Cock Harlen Torres-Castañeda Omar V. Pabón-Rodríguez Carlos E. Agudelo-Morales Nathiely Ramírez-Guzmán Juan A. Ascacio-Valdés Cristóbal N. Aguilar Cristian Torres-León |
| author_facet | Sarah L. Paz-Arteaga Edith Cadena-Chamorro Liliana Serna-Cock Harlen Torres-Castañeda Omar V. Pabón-Rodríguez Carlos E. Agudelo-Morales Nathiely Ramírez-Guzmán Juan A. Ascacio-Valdés Cristóbal N. Aguilar Cristian Torres-León |
| author_sort | Sarah L. Paz-Arteaga |
| collection | DOAJ |
| description | The exploration of natural antimicrobial compounds is necessary due to the current bacterial resistance to synthetic antibiotics. For this reason, pineapple residues were evaluated as a natural source of phenolic compounds with antimicrobial capacity. Pineapple residues were fermented with <i>A. niger</i> GH1 and subjected to ultrasound for the extraction of phenolic compounds. In the solid-state fermentation (SSF), the peel and the core (70:30) were fermented for 32 h. Ultrasonic-assisted extraction (UAE) was optimized. The factors solid–liquid ratio (X1), ethanol concentration (X2), and extraction cycle (X3) were optimized through a Box–Behnken statistical design, using the total phenol content (TPC) as a response variable. The optimized and pre-purified extract with amberlite was characterized using HPLC-MS and we measured the antimicrobial activity against <i>Listeria monocytogenes</i>, <i>Staphylococcus aureus</i>, and <i>Escherichia coli</i>. The optimal values for X1, X2, and X3 were 82 mg/mL, 26%, and two cycles, respectively. The extract was effective as an antimicrobial agent against <i>S. aureus</i> and <i>L. monocytogenes</i>, with MICs of 50 mg/mL and 12.5 mg/mL, respectively; the bacterial growth curve and transmission electron microscopy (TEM) confirmed the antimicrobial activity. The main compounds identified in the antimicrobial extract were 3,4-DHPEA-EA, Phloretin 2′-O-xylosyl-glucoside, and Feruloyl tartaric acid. Overall, the combination SSF-UAE showed to be a promising strategy to recover phenolic compounds from pineapple residues, exhibiting great potential as a natural antimicrobial agent. |
| first_indexed | 2024-03-10T23:57:13Z |
| format | Article |
| id | doaj.art-5bd01c672d9e4a5da0364a0a443fb88b |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-10T23:57:13Z |
| publishDate | 2023-07-01 |
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| series | Fermentation |
| spelling | doaj.art-5bd01c672d9e4a5da0364a0a443fb88b2023-11-19T01:01:15ZengMDPI AGFermentation2311-56372023-07-019870610.3390/fermentation9080706Dual Emerging Applications of Solid-State Fermentation (SSF) with <em>Aspergillus niger</em> and Ultrasonic-Assisted Extraction (UAE) for the Obtention of Antimicrobial Polyphenols from Pineapple WasteSarah L. Paz-Arteaga0Edith Cadena-Chamorro1Liliana Serna-Cock2Harlen Torres-Castañeda3Omar V. Pabón-Rodríguez4Carlos E. Agudelo-Morales5Nathiely Ramírez-Guzmán6Juan A. Ascacio-Valdés7Cristóbal N. Aguilar8Cristian Torres-León9Faculty of Sciences, Universidad Nacional de Colombia, Medellín 050034, ColombiaFaculty of Agricultural Sciences, Universidad Nacional de Colombia, Medellín 050034, ColombiaFaculty of Engineering and Administration, Universidad Nacional de Colombia, Palmira 763533, ColombiaFaculty of Engineering and Administration, Universidad Nacional de Colombia, Palmira 763533, ColombiaFaculty of Engineering and Administration, Universidad Nacional de Colombia, Palmira 763533, ColombiaFaculty of Agricultural Sciences, Universidad Nacional de Colombia, Palmira 763533, ColombiaCenter for Interdisciplinary Studies and Research, Universidad Autonoma de Coahuila, Saltillo 25280, MexicoBioprocesses & Bioproducts Research Group, School of Chemistry, Universidad Autonoma de Coahuila, Unidad Saltillo, Saltillo 25280, MexicoBioprocesses & Bioproducts Research Group, School of Chemistry, Universidad Autonoma de Coahuila, Unidad Saltillo, Saltillo 25280, MexicoResearch Center and Ethnobiological Garden (CIJE), Universidad Autonoma de Coahuila, Unidad Torreón, Viesca 27480, MexicoThe exploration of natural antimicrobial compounds is necessary due to the current bacterial resistance to synthetic antibiotics. For this reason, pineapple residues were evaluated as a natural source of phenolic compounds with antimicrobial capacity. Pineapple residues were fermented with <i>A. niger</i> GH1 and subjected to ultrasound for the extraction of phenolic compounds. In the solid-state fermentation (SSF), the peel and the core (70:30) were fermented for 32 h. Ultrasonic-assisted extraction (UAE) was optimized. The factors solid–liquid ratio (X1), ethanol concentration (X2), and extraction cycle (X3) were optimized through a Box–Behnken statistical design, using the total phenol content (TPC) as a response variable. The optimized and pre-purified extract with amberlite was characterized using HPLC-MS and we measured the antimicrobial activity against <i>Listeria monocytogenes</i>, <i>Staphylococcus aureus</i>, and <i>Escherichia coli</i>. The optimal values for X1, X2, and X3 were 82 mg/mL, 26%, and two cycles, respectively. The extract was effective as an antimicrobial agent against <i>S. aureus</i> and <i>L. monocytogenes</i>, with MICs of 50 mg/mL and 12.5 mg/mL, respectively; the bacterial growth curve and transmission electron microscopy (TEM) confirmed the antimicrobial activity. The main compounds identified in the antimicrobial extract were 3,4-DHPEA-EA, Phloretin 2′-O-xylosyl-glucoside, and Feruloyl tartaric acid. Overall, the combination SSF-UAE showed to be a promising strategy to recover phenolic compounds from pineapple residues, exhibiting great potential as a natural antimicrobial agent.https://www.mdpi.com/2311-5637/9/8/706pineapple by-productsbioactive compoundsphenolic compoundsbiological propertiesantimicrobial activity |
| spellingShingle | Sarah L. Paz-Arteaga Edith Cadena-Chamorro Liliana Serna-Cock Harlen Torres-Castañeda Omar V. Pabón-Rodríguez Carlos E. Agudelo-Morales Nathiely Ramírez-Guzmán Juan A. Ascacio-Valdés Cristóbal N. Aguilar Cristian Torres-León Dual Emerging Applications of Solid-State Fermentation (SSF) with <em>Aspergillus niger</em> and Ultrasonic-Assisted Extraction (UAE) for the Obtention of Antimicrobial Polyphenols from Pineapple Waste Fermentation pineapple by-products bioactive compounds phenolic compounds biological properties antimicrobial activity |
| title | Dual Emerging Applications of Solid-State Fermentation (SSF) with <em>Aspergillus niger</em> and Ultrasonic-Assisted Extraction (UAE) for the Obtention of Antimicrobial Polyphenols from Pineapple Waste |
| title_full | Dual Emerging Applications of Solid-State Fermentation (SSF) with <em>Aspergillus niger</em> and Ultrasonic-Assisted Extraction (UAE) for the Obtention of Antimicrobial Polyphenols from Pineapple Waste |
| title_fullStr | Dual Emerging Applications of Solid-State Fermentation (SSF) with <em>Aspergillus niger</em> and Ultrasonic-Assisted Extraction (UAE) for the Obtention of Antimicrobial Polyphenols from Pineapple Waste |
| title_full_unstemmed | Dual Emerging Applications of Solid-State Fermentation (SSF) with <em>Aspergillus niger</em> and Ultrasonic-Assisted Extraction (UAE) for the Obtention of Antimicrobial Polyphenols from Pineapple Waste |
| title_short | Dual Emerging Applications of Solid-State Fermentation (SSF) with <em>Aspergillus niger</em> and Ultrasonic-Assisted Extraction (UAE) for the Obtention of Antimicrobial Polyphenols from Pineapple Waste |
| title_sort | dual emerging applications of solid state fermentation ssf with em aspergillus niger em and ultrasonic assisted extraction uae for the obtention of antimicrobial polyphenols from pineapple waste |
| topic | pineapple by-products bioactive compounds phenolic compounds biological properties antimicrobial activity |
| url | https://www.mdpi.com/2311-5637/9/8/706 |
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