Gene Expression in MC3T3-E1 Cells Treated with Diclofenac and Methylprednisolone
Nonsteroidal anti-inflammatory drugs (NSAIDs) and glucocorticoids (GCs) are often used to treat articular-skeletal disorders. The extended use of NSAIDs and GCs have adverse effects on bone metabolism, reducing bone quality and impairing fracture healing. In the present study, we used mouse pre-oste...
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MDPI AG
2023-01-01
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| Online Access: | https://www.mdpi.com/2073-4425/14/1/184 |
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| author | Tomasz P. Lehmann Ewa Iwańczyk-Skalska Jerzy Harasymczuk Paweł P. Jagodziński Maciej Głowacki |
| author_facet | Tomasz P. Lehmann Ewa Iwańczyk-Skalska Jerzy Harasymczuk Paweł P. Jagodziński Maciej Głowacki |
| author_sort | Tomasz P. Lehmann |
| collection | DOAJ |
| description | Nonsteroidal anti-inflammatory drugs (NSAIDs) and glucocorticoids (GCs) are often used to treat articular-skeletal disorders. The extended use of NSAIDs and GCs have adverse effects on bone metabolism, reducing bone quality and impairing fracture healing. In the present study, we used mouse pre-osteoblast cells MC3T3-E1 to demonstrate the effects of diclofenac (DF) and methylprednisolone (MP) on cell proliferation and gene expression. Cells were incubated with three doses of DF or MP: 0.5 µM, 5 µM, and 50 µM. MP decreased cell viability even after 24 h, but DF inhibited cell viability after only seven days of treatment. The cells were lysed after one, two, three, and seven days of treatment, and gene expression was analyzed by reverse transcription and quantitative PCR (RT-qPCR) assays. DF did not significantly affect the expression of the osteogenic marker genes. MP modified the expression of <i>Osx</i>, <i>Runx</i>, and <i>Col1a1</i>. We concluded that MP is a more potent inhibitor of mouse pre-osteoblast differentiation and viability than is DF. Our results suggest that prolonged DF treatment could be less harmful to osteoblasts than MP treatment. |
| first_indexed | 2024-03-09T12:35:51Z |
| format | Article |
| id | doaj.art-5bd0fe6c02674a11a6eb00e6e580b9fd |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T12:35:51Z |
| publishDate | 2023-01-01 |
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| record_format | Article |
| series | Genes |
| spelling | doaj.art-5bd0fe6c02674a11a6eb00e6e580b9fd2023-11-30T22:24:50ZengMDPI AGGenes2073-44252023-01-0114118410.3390/genes14010184Gene Expression in MC3T3-E1 Cells Treated with Diclofenac and MethylprednisoloneTomasz P. Lehmann0Ewa Iwańczyk-Skalska1Jerzy Harasymczuk2Paweł P. Jagodziński3Maciej Głowacki4Department of Biochemistry and Molecular Biology, Poznań University of Medical Sciences, Święcickiego 6, 60-781 Poznan, PolandDepartment of Biochemistry and Molecular Biology, Poznań University of Medical Sciences, Święcickiego 6, 60-781 Poznan, PolandDepartment of Paediatric Surgery, Traumatology and Urology, Poznan University of Medical Sciences, Szpitalna 27/33, 60-572 Poznan, PolandDepartment of Biochemistry and Molecular Biology, Poznań University of Medical Sciences, Święcickiego 6, 60-781 Poznan, PolandDepartment of Paediatric Orthopaedics and Traumatology, Poznan University of Medical Sciences, 28 Czerwca 1956 135/147, 61-545 Poznan, PolandNonsteroidal anti-inflammatory drugs (NSAIDs) and glucocorticoids (GCs) are often used to treat articular-skeletal disorders. The extended use of NSAIDs and GCs have adverse effects on bone metabolism, reducing bone quality and impairing fracture healing. In the present study, we used mouse pre-osteoblast cells MC3T3-E1 to demonstrate the effects of diclofenac (DF) and methylprednisolone (MP) on cell proliferation and gene expression. Cells were incubated with three doses of DF or MP: 0.5 µM, 5 µM, and 50 µM. MP decreased cell viability even after 24 h, but DF inhibited cell viability after only seven days of treatment. The cells were lysed after one, two, three, and seven days of treatment, and gene expression was analyzed by reverse transcription and quantitative PCR (RT-qPCR) assays. DF did not significantly affect the expression of the osteogenic marker genes. MP modified the expression of <i>Osx</i>, <i>Runx</i>, and <i>Col1a1</i>. We concluded that MP is a more potent inhibitor of mouse pre-osteoblast differentiation and viability than is DF. Our results suggest that prolonged DF treatment could be less harmful to osteoblasts than MP treatment.https://www.mdpi.com/2073-4425/14/1/184MC3T3diclofenacmethylprednisoloneglucocorticoidnonsteroidal anti-inflammatory drugs |
| spellingShingle | Tomasz P. Lehmann Ewa Iwańczyk-Skalska Jerzy Harasymczuk Paweł P. Jagodziński Maciej Głowacki Gene Expression in MC3T3-E1 Cells Treated with Diclofenac and Methylprednisolone Genes MC3T3 diclofenac methylprednisolone glucocorticoid nonsteroidal anti-inflammatory drugs |
| title | Gene Expression in MC3T3-E1 Cells Treated with Diclofenac and Methylprednisolone |
| title_full | Gene Expression in MC3T3-E1 Cells Treated with Diclofenac and Methylprednisolone |
| title_fullStr | Gene Expression in MC3T3-E1 Cells Treated with Diclofenac and Methylprednisolone |
| title_full_unstemmed | Gene Expression in MC3T3-E1 Cells Treated with Diclofenac and Methylprednisolone |
| title_short | Gene Expression in MC3T3-E1 Cells Treated with Diclofenac and Methylprednisolone |
| title_sort | gene expression in mc3t3 e1 cells treated with diclofenac and methylprednisolone |
| topic | MC3T3 diclofenac methylprednisolone glucocorticoid nonsteroidal anti-inflammatory drugs |
| url | https://www.mdpi.com/2073-4425/14/1/184 |
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