The effects of resveratrol and melatonin on biochemical and molecular parameters in diabetic old female rat hearts
The roles of melatonin and resveratrol-enhanced activation of SIRT1 (silent information regulator 1), GLUT4 (glucose transporter type 4), and PGC-1α (peroxisome proliferator-activated receptor gamma coactivator 1-alpha) in mediating the protective effects on the heart in aged female rats with strept...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-02-01
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| Series: | Experimental Gerontology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0531556522003527 |
| _version_ | 1827897297169547264 |
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| author | Nilufer Akgun-Unal Serhan Ozyildirim Omer Unal Elif Gulbahce-Mutlu Rasim Mogulkoc Abdulkerim Kasim Baltaci |
| author_facet | Nilufer Akgun-Unal Serhan Ozyildirim Omer Unal Elif Gulbahce-Mutlu Rasim Mogulkoc Abdulkerim Kasim Baltaci |
| author_sort | Nilufer Akgun-Unal |
| collection | DOAJ |
| description | The roles of melatonin and resveratrol-enhanced activation of SIRT1 (silent information regulator 1), GLUT4 (glucose transporter type 4), and PGC-1α (peroxisome proliferator-activated receptor gamma coactivator 1-alpha) in mediating the protective effects on the heart in aged female rats with streptozotocin-induced diabetes were investigated. 16-month-old 48 Wistar female rats were separated into 8 groups with equal numbers. Group 1: Control, Group 2: Resveratrol Control, Group 3: Melatonin Control, Group 4: Resveratrol and Melatonin Control, Group 5: Diabetes, Group 6: Diabetes Resveratrol, Group 7: Diabetes Melatonin, Group 8: Diabetes Resveratrol and Melatonin. A single dose of 40 mg/kg intraperitoneal streptozotocin was injected into the rats of Groups 5, 6, 7, and 8 to induce experimental diabetes. Blood glucose levels were measured from the tail veins of the animals six days after the injections, using a diagnostic glucose kit. Rats with a blood glucose levels ≥300 mg/dl were considered diabetic. 5 mg/kg/day of resveratrol (intraperitoneal) and melatonin (subcutaneous) were administered for four weeks. At the end of the applications, SIRT1, GLUT4, PGC-1α gene expression as well as MDA and GSH levels in the heart tissues were determined by the PCR method from heart tissue samples taken under general anesthesia.The findings of our study show that suppressed antioxidant activity and decreased GLUT4, SIRT1, and PGC-1α gene expression in heart tissue can be reversed by the combination of resveratrol, melatonin, and resveratrol + melatonin in a diabetic aged female rat model. Resveratrol and melatonin supplementation may have a protective effect on cardiac functions in the diabetic aged female rat model. |
| first_indexed | 2024-03-12T22:47:07Z |
| format | Article |
| id | doaj.art-5bd16928a27547a7beb3467572c2a20f |
| institution | Directory Open Access Journal |
| issn | 1873-6815 |
| language | English |
| last_indexed | 2024-03-12T22:47:07Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Experimental Gerontology |
| spelling | doaj.art-5bd16928a27547a7beb3467572c2a20f2023-07-21T04:58:37ZengElsevierExperimental Gerontology1873-68152023-02-01172112043The effects of resveratrol and melatonin on biochemical and molecular parameters in diabetic old female rat heartsNilufer Akgun-Unal0Serhan Ozyildirim1Omer Unal2Elif Gulbahce-Mutlu3Rasim Mogulkoc4Abdulkerim Kasim Baltaci5Department of Biophysics, Medicine Faculty, Ondokuz Mayis University, Samsun, Turkey; Corresponding author.Department of Cardiology, Institution of Cardiology, Istanbul University-Cerrahpasa, Istanbul, TurkeyDepartment of Physiology, Medical Faculty, Kirikkale University, Kirikkale, TurkeyDepartment of Medical Biology, Medical Faculty, KTO Karatay University, Konya, TurkeyDepartment of Physiology, Medical Faculty, Selcuk University, Konya, TurkeyDepartment of Physiology, Medical Faculty, Selcuk University, Konya, TurkeyThe roles of melatonin and resveratrol-enhanced activation of SIRT1 (silent information regulator 1), GLUT4 (glucose transporter type 4), and PGC-1α (peroxisome proliferator-activated receptor gamma coactivator 1-alpha) in mediating the protective effects on the heart in aged female rats with streptozotocin-induced diabetes were investigated. 16-month-old 48 Wistar female rats were separated into 8 groups with equal numbers. Group 1: Control, Group 2: Resveratrol Control, Group 3: Melatonin Control, Group 4: Resveratrol and Melatonin Control, Group 5: Diabetes, Group 6: Diabetes Resveratrol, Group 7: Diabetes Melatonin, Group 8: Diabetes Resveratrol and Melatonin. A single dose of 40 mg/kg intraperitoneal streptozotocin was injected into the rats of Groups 5, 6, 7, and 8 to induce experimental diabetes. Blood glucose levels were measured from the tail veins of the animals six days after the injections, using a diagnostic glucose kit. Rats with a blood glucose levels ≥300 mg/dl were considered diabetic. 5 mg/kg/day of resveratrol (intraperitoneal) and melatonin (subcutaneous) were administered for four weeks. At the end of the applications, SIRT1, GLUT4, PGC-1α gene expression as well as MDA and GSH levels in the heart tissues were determined by the PCR method from heart tissue samples taken under general anesthesia.The findings of our study show that suppressed antioxidant activity and decreased GLUT4, SIRT1, and PGC-1α gene expression in heart tissue can be reversed by the combination of resveratrol, melatonin, and resveratrol + melatonin in a diabetic aged female rat model. Resveratrol and melatonin supplementation may have a protective effect on cardiac functions in the diabetic aged female rat model.http://www.sciencedirect.com/science/article/pii/S0531556522003527DiabetesMelatoninResveratrolAged female ratAntioxidant |
| spellingShingle | Nilufer Akgun-Unal Serhan Ozyildirim Omer Unal Elif Gulbahce-Mutlu Rasim Mogulkoc Abdulkerim Kasim Baltaci The effects of resveratrol and melatonin on biochemical and molecular parameters in diabetic old female rat hearts Experimental Gerontology Diabetes Melatonin Resveratrol Aged female rat Antioxidant |
| title | The effects of resveratrol and melatonin on biochemical and molecular parameters in diabetic old female rat hearts |
| title_full | The effects of resveratrol and melatonin on biochemical and molecular parameters in diabetic old female rat hearts |
| title_fullStr | The effects of resveratrol and melatonin on biochemical and molecular parameters in diabetic old female rat hearts |
| title_full_unstemmed | The effects of resveratrol and melatonin on biochemical and molecular parameters in diabetic old female rat hearts |
| title_short | The effects of resveratrol and melatonin on biochemical and molecular parameters in diabetic old female rat hearts |
| title_sort | effects of resveratrol and melatonin on biochemical and molecular parameters in diabetic old female rat hearts |
| topic | Diabetes Melatonin Resveratrol Aged female rat Antioxidant |
| url | http://www.sciencedirect.com/science/article/pii/S0531556522003527 |
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