Spatio-temporal connectivity of the aquatic microbiome associated with cyanobacterial blooms along a Great Lake riverine-lacustrine continuum
Lake Erie is subject to recurring events of cyanobacterial harmful algal blooms (cHABs), but measures of nutrients and total phytoplankton biomass seem to be poor predictors of cHABs when taken individually. A more integrated approach at the watershed scale may improve our understanding of the condi...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1073753/full |
| _version_ | 1811168280874844160 |
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| author | Sophie Crevecoeur Thomas A. Edge Linet Cynthia Watson Susan B. Watson Charles W. Greer Jan J. H. Ciborowski Jan J. H. Ciborowski Ngan Diep Alice Dove Kenneth G. Drouillard Thijs Frenken Thijs Frenken Robert Michael McKay Robert Michael McKay Arthur Zastepa Jérôme Comte Jérôme Comte |
| author_facet | Sophie Crevecoeur Thomas A. Edge Linet Cynthia Watson Susan B. Watson Charles W. Greer Jan J. H. Ciborowski Jan J. H. Ciborowski Ngan Diep Alice Dove Kenneth G. Drouillard Thijs Frenken Thijs Frenken Robert Michael McKay Robert Michael McKay Arthur Zastepa Jérôme Comte Jérôme Comte |
| author_sort | Sophie Crevecoeur |
| collection | DOAJ |
| description | Lake Erie is subject to recurring events of cyanobacterial harmful algal blooms (cHABs), but measures of nutrients and total phytoplankton biomass seem to be poor predictors of cHABs when taken individually. A more integrated approach at the watershed scale may improve our understanding of the conditions that lead to bloom formation, such as assessing the physico-chemical and biological factors that influence the lake microbial community, as well as identifying the linkages between Lake Erie and the surrounding watershed. Within the scope of the Government of Canada’s Genomics Research and Development Initiative (GRDI) Ecobiomics project, we used high-throughput sequencing of the 16S rRNA gene to characterize the spatio-temporal variability of the aquatic microbiome in the Thames River–Lake St. Clair-Detroit River–Lake Erie aquatic corridor. We found that the aquatic microbiome was structured along the flow path and influenced mainly by higher nutrient concentrations in the Thames River, and higher temperature and pH downstream in Lake St. Clair and Lake Erie. The same dominant bacterial phyla were detected along the water continuum, changing only in relative abundance. At finer taxonomical level, however, there was a clear shift in the cyanobacterial community, with Planktothrix dominating in the Thames River and Microcystis and Synechococcus in Lake St. Clair and Lake Erie. Mantel correlations highlighted the importance of geographic distance in shaping the microbial community structure. The fact that a high proportion of microbial sequences found in the Western Basin of Lake Erie were also identified in the Thames River, indicated a high degree of connectivity and dispersal within the system, where mass effect induced by passive transport play an important role in microbial community assembly. Nevertheless, some cyanobacterial amplicon sequence variants (ASVs) related to Microcystis, representing less than 0.1% of relative abundance in the upstream Thames River, became dominant in Lake St. Clair and Erie, suggesting selection of those ASVs based on the lake conditions. Their extremely low relative abundances in the Thames suggest additional sources are likely to contribute to the rapid development of summer and fall blooms in the Western Basin of Lake Erie. Collectively, these results, which can be applied to other watersheds, improve our understanding of the factors influencing aquatic microbial community assembly and provide new perspectives on how to better understand the occurrence of cHABs in Lake Erie and elsewhere. |
| first_indexed | 2024-04-10T16:23:30Z |
| format | Article |
| id | doaj.art-5bd1f8bc37e44a28ab2578447a836940 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T16:23:30Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-5bd1f8bc37e44a28ab2578447a8369402023-02-09T09:49:13ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-02-011410.3389/fmicb.2023.10737531073753Spatio-temporal connectivity of the aquatic microbiome associated with cyanobacterial blooms along a Great Lake riverine-lacustrine continuumSophie Crevecoeur0Thomas A. Edge1Linet Cynthia Watson2Susan B. Watson3Charles W. Greer4Jan J. H. Ciborowski5Jan J. H. Ciborowski6Ngan Diep7Alice Dove8Kenneth G. Drouillard9Thijs Frenken10Thijs Frenken11Robert Michael McKay12Robert Michael McKay13Arthur Zastepa14Jérôme Comte15Jérôme Comte16Watershed Hydrology and Ecology Research Division, Environment and Climate Change Canada, Burlington, ON, CanadaDepartment of Biology, McMaster University, Hamilton, ON, CanadaWatershed Hydrology and Ecology Research Division, Environment and Climate Change Canada, Burlington, ON, CanadaDepartment of Biology, Trent University, Peterborough, ON, CanadaEnergy, Mining and Environment, National Research Council of Canada, Montreal, QC, CanadaDepartment of Integrative Biology, University of Windsor, Windsor, ON, CanadaDepartment of Biological Sciences University of Calgary, Calgary, AB, CanadaOntario Ministry of the Environment, Conservation and Parks, Environmental Monitoring and Reporting Branch, Etobicoke, ON, CanadaWatershed Hydrology and Ecology Research Division, Environment and Climate Change Canada, Burlington, ON, CanadaGreat Lakes Institute for Environmental Research, University of Windsor, Windsor, ON, CanadaGreat Lakes Institute for Environmental Research, University of Windsor, Windsor, ON, CanadaCluster Nature & Society, HAS University of Applied Sciences, s-Hertogenbosch, NetherlandsGreat Lakes Institute for Environmental Research, University of Windsor, Windsor, ON, Canada0Great Lakes Center for Fresh Waters and Human Health, Bowling Green State University, Bowling Green, OH, United StatesWatershed Hydrology and Ecology Research Division, Environment and Climate Change Canada, Burlington, ON, Canada1Centre Eau Terre Environnement, Institut National de la Recherche Scientifique, Quebec City, QC, Canada2Groupe de Recherche Interuniversitaire en Limnologie et en Environnement Aquatique (GRIL), Université de Montréal, Montreal, QC, CanadaLake Erie is subject to recurring events of cyanobacterial harmful algal blooms (cHABs), but measures of nutrients and total phytoplankton biomass seem to be poor predictors of cHABs when taken individually. A more integrated approach at the watershed scale may improve our understanding of the conditions that lead to bloom formation, such as assessing the physico-chemical and biological factors that influence the lake microbial community, as well as identifying the linkages between Lake Erie and the surrounding watershed. Within the scope of the Government of Canada’s Genomics Research and Development Initiative (GRDI) Ecobiomics project, we used high-throughput sequencing of the 16S rRNA gene to characterize the spatio-temporal variability of the aquatic microbiome in the Thames River–Lake St. Clair-Detroit River–Lake Erie aquatic corridor. We found that the aquatic microbiome was structured along the flow path and influenced mainly by higher nutrient concentrations in the Thames River, and higher temperature and pH downstream in Lake St. Clair and Lake Erie. The same dominant bacterial phyla were detected along the water continuum, changing only in relative abundance. At finer taxonomical level, however, there was a clear shift in the cyanobacterial community, with Planktothrix dominating in the Thames River and Microcystis and Synechococcus in Lake St. Clair and Lake Erie. Mantel correlations highlighted the importance of geographic distance in shaping the microbial community structure. The fact that a high proportion of microbial sequences found in the Western Basin of Lake Erie were also identified in the Thames River, indicated a high degree of connectivity and dispersal within the system, where mass effect induced by passive transport play an important role in microbial community assembly. Nevertheless, some cyanobacterial amplicon sequence variants (ASVs) related to Microcystis, representing less than 0.1% of relative abundance in the upstream Thames River, became dominant in Lake St. Clair and Erie, suggesting selection of those ASVs based on the lake conditions. Their extremely low relative abundances in the Thames suggest additional sources are likely to contribute to the rapid development of summer and fall blooms in the Western Basin of Lake Erie. Collectively, these results, which can be applied to other watersheds, improve our understanding of the factors influencing aquatic microbial community assembly and provide new perspectives on how to better understand the occurrence of cHABs in Lake Erie and elsewhere.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1073753/fullaquatic microbiomecyanobacteriaLake Erie watershedharmful algal bloomsgenetic connectivity |
| spellingShingle | Sophie Crevecoeur Thomas A. Edge Linet Cynthia Watson Susan B. Watson Charles W. Greer Jan J. H. Ciborowski Jan J. H. Ciborowski Ngan Diep Alice Dove Kenneth G. Drouillard Thijs Frenken Thijs Frenken Robert Michael McKay Robert Michael McKay Arthur Zastepa Jérôme Comte Jérôme Comte Spatio-temporal connectivity of the aquatic microbiome associated with cyanobacterial blooms along a Great Lake riverine-lacustrine continuum Frontiers in Microbiology aquatic microbiome cyanobacteria Lake Erie watershed harmful algal blooms genetic connectivity |
| title | Spatio-temporal connectivity of the aquatic microbiome associated with cyanobacterial blooms along a Great Lake riverine-lacustrine continuum |
| title_full | Spatio-temporal connectivity of the aquatic microbiome associated with cyanobacterial blooms along a Great Lake riverine-lacustrine continuum |
| title_fullStr | Spatio-temporal connectivity of the aquatic microbiome associated with cyanobacterial blooms along a Great Lake riverine-lacustrine continuum |
| title_full_unstemmed | Spatio-temporal connectivity of the aquatic microbiome associated with cyanobacterial blooms along a Great Lake riverine-lacustrine continuum |
| title_short | Spatio-temporal connectivity of the aquatic microbiome associated with cyanobacterial blooms along a Great Lake riverine-lacustrine continuum |
| title_sort | spatio temporal connectivity of the aquatic microbiome associated with cyanobacterial blooms along a great lake riverine lacustrine continuum |
| topic | aquatic microbiome cyanobacteria Lake Erie watershed harmful algal blooms genetic connectivity |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1073753/full |
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