Maturation of phagosomes containing different erythrophagocytic particles in primary macrophages
Erythrophagocytosis is a physiological process that aims to remove damaged red blood cells from the circulation in order to avoid hemolysis and uncontrolled liberation of iron. Many efforts have been made to understand heme trafficking inside macrophages, but little is known about the maturation of...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-09-01
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| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/2211-5463.12262 |
| _version_ | 1797989094272270336 |
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| author | Inês B. Santarino Otília V. Vieira |
| author_facet | Inês B. Santarino Otília V. Vieira |
| author_sort | Inês B. Santarino |
| collection | DOAJ |
| description | Erythrophagocytosis is a physiological process that aims to remove damaged red blood cells from the circulation in order to avoid hemolysis and uncontrolled liberation of iron. Many efforts have been made to understand heme trafficking inside macrophages, but little is known about the maturation of phagosomes containing different types of erythrophagocytic particles with different signals at their surfaces. Therefore, we performed a comparative study on the maturation of phagosomes containing three different models of red blood cells (RBC): aged/senescent, complement‐opsonized, and IgG‐opsonized. We also used two types of professional phagocytes: bone marrow‐derived and peritoneal macrophages. By comparing markers from different stages of phagosomal maturation, we found that phagosomes carrying aged RBC reach lysosomes with a delay compared to those containing IgG‐ or complement‐opsonized RBC, in both types of macrophages. These findings contribute to understanding the importance of the different signals at the RBC surface in phagolysosome biogenesis, as well as in the dynamics of RBC removal. |
| first_indexed | 2024-04-11T08:13:40Z |
| format | Article |
| id | doaj.art-5bd60f99f0814e8c88b719a8b5963fac |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-04-11T08:13:40Z |
| publishDate | 2017-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-5bd60f99f0814e8c88b719a8b5963fac2022-12-22T04:35:14ZengWileyFEBS Open Bio2211-54632017-09-01791281129010.1002/2211-5463.12262Maturation of phagosomes containing different erythrophagocytic particles in primary macrophagesInês B. Santarino0Otília V. Vieira1CEDOC NOVA Medical School, Faculdade de Ciências Médicas Universidade NOVA de Lisboa PortugalCEDOC NOVA Medical School, Faculdade de Ciências Médicas Universidade NOVA de Lisboa PortugalErythrophagocytosis is a physiological process that aims to remove damaged red blood cells from the circulation in order to avoid hemolysis and uncontrolled liberation of iron. Many efforts have been made to understand heme trafficking inside macrophages, but little is known about the maturation of phagosomes containing different types of erythrophagocytic particles with different signals at their surfaces. Therefore, we performed a comparative study on the maturation of phagosomes containing three different models of red blood cells (RBC): aged/senescent, complement‐opsonized, and IgG‐opsonized. We also used two types of professional phagocytes: bone marrow‐derived and peritoneal macrophages. By comparing markers from different stages of phagosomal maturation, we found that phagosomes carrying aged RBC reach lysosomes with a delay compared to those containing IgG‐ or complement‐opsonized RBC, in both types of macrophages. These findings contribute to understanding the importance of the different signals at the RBC surface in phagolysosome biogenesis, as well as in the dynamics of RBC removal.https://doi.org/10.1002/2211-5463.12262complement‐opsonized red blood cells, aged red blood cellserythrophagocytosisIgG‐opsonized Red Blood Cellsphagosomal maturation |
| spellingShingle | Inês B. Santarino Otília V. Vieira Maturation of phagosomes containing different erythrophagocytic particles in primary macrophages FEBS Open Bio complement‐opsonized red blood cells, aged red blood cells erythrophagocytosis IgG‐opsonized Red Blood Cells phagosomal maturation |
| title | Maturation of phagosomes containing different erythrophagocytic particles in primary macrophages |
| title_full | Maturation of phagosomes containing different erythrophagocytic particles in primary macrophages |
| title_fullStr | Maturation of phagosomes containing different erythrophagocytic particles in primary macrophages |
| title_full_unstemmed | Maturation of phagosomes containing different erythrophagocytic particles in primary macrophages |
| title_short | Maturation of phagosomes containing different erythrophagocytic particles in primary macrophages |
| title_sort | maturation of phagosomes containing different erythrophagocytic particles in primary macrophages |
| topic | complement‐opsonized red blood cells, aged red blood cells erythrophagocytosis IgG‐opsonized Red Blood Cells phagosomal maturation |
| url | https://doi.org/10.1002/2211-5463.12262 |
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