Milk fat globule membrane protects Bifidobacterium longum ssp. infantis ATCC 15697 against bile stress by modifying global transcriptional responses
ABSTRACT: The milk fat globule membrane (MFGM) can protect probiotic bacteria from bile stress. However, its potential mechanism has not been reported. In this study, the viability, morphology and gene transcriptional response of Bifidobacterium longum ssp. infantis ATCC 15697 (BI_15697) stressed by...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Journal of Dairy Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022030223006082 |
| _version_ | 1797356122835779584 |
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| author | Gongsheng Zhang Mingxue He Lihong Xiao Yuehua Jiao Jianchun Han Chun Li Michael J. Miller Lili Zhang |
| author_facet | Gongsheng Zhang Mingxue He Lihong Xiao Yuehua Jiao Jianchun Han Chun Li Michael J. Miller Lili Zhang |
| author_sort | Gongsheng Zhang |
| collection | DOAJ |
| description | ABSTRACT: The milk fat globule membrane (MFGM) can protect probiotic bacteria from bile stress. However, its potential mechanism has not been reported. In this study, the viability, morphology and gene transcriptional response of Bifidobacterium longum ssp. infantis ATCC 15697 (BI_15697) stressed by bile salts with or without MFGM were investigated. It was shown that MFGM alleviated the reduction in BI_15697 population induced by 0.2% porcine bile stress and restored the population to the control levels. MFGM ameliorated the shrunken, fragmented appearance and irregular morphology of BI_15697 and maintained cell integrity disrupted by bile stress. RNA-sequencing results showed that MFGM increased transport of glucose and raffinose and decreased that of branched-chain amino acids (BCAA) in the presence of bile salts. MFGM stimulated the expression of genes involved in the synthesis of raffinose in galactose metabolism and the metabolism of BCAA, suggesting that MFGM stimulated the accumulation of raffinose and BCAA in the presence of bile. In addition, MFGM stimulated the expression of 2 bile efflux transporters under bile stress. Together, the multifactorial response helps BI_15697 excrete bile salts and maintain cellular integrity in response to bile stress. This study proposes a mechanism for the protection of BI_15697 against bile salt stress by MFGM, thereby providing a molecular basis for its application in incorporation of probiotics. |
| first_indexed | 2024-03-08T14:22:11Z |
| format | Article |
| id | doaj.art-5bd689181a754b1e8d1c87c5ad77077a |
| institution | Directory Open Access Journal |
| issn | 0022-0302 |
| language | English |
| last_indexed | 2024-03-08T14:22:11Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dairy Science |
| spelling | doaj.art-5bd689181a754b1e8d1c87c5ad77077a2024-01-14T05:34:45ZengElsevierJournal of Dairy Science0022-03022024-01-01107191104Milk fat globule membrane protects Bifidobacterium longum ssp. infantis ATCC 15697 against bile stress by modifying global transcriptional responsesGongsheng Zhang0Mingxue He1Lihong Xiao2Yuehua Jiao3Jianchun Han4Chun Li5Michael J. Miller6Lili Zhang7Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin 150030, ChinaCenter of Drug Safety Evaluation, Heilongjiang University of Chinese Medicine, Harbin 150040, ChinaKey Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin 150030, China; Heilongjiang Green Food Science Research Institute, Harbin 150030, China; Corresponding authorsKey Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin 150030, China; Center of Drug Safety Evaluation, Heilongjiang University of Chinese Medicine, Harbin 150040, ChinaDepartment of Food Science and Human Nutrition, University of Illinois Urbana-Champaign, Urbana, IL 61801Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin 150030, China; Corresponding authorsABSTRACT: The milk fat globule membrane (MFGM) can protect probiotic bacteria from bile stress. However, its potential mechanism has not been reported. In this study, the viability, morphology and gene transcriptional response of Bifidobacterium longum ssp. infantis ATCC 15697 (BI_15697) stressed by bile salts with or without MFGM were investigated. It was shown that MFGM alleviated the reduction in BI_15697 population induced by 0.2% porcine bile stress and restored the population to the control levels. MFGM ameliorated the shrunken, fragmented appearance and irregular morphology of BI_15697 and maintained cell integrity disrupted by bile stress. RNA-sequencing results showed that MFGM increased transport of glucose and raffinose and decreased that of branched-chain amino acids (BCAA) in the presence of bile salts. MFGM stimulated the expression of genes involved in the synthesis of raffinose in galactose metabolism and the metabolism of BCAA, suggesting that MFGM stimulated the accumulation of raffinose and BCAA in the presence of bile. In addition, MFGM stimulated the expression of 2 bile efflux transporters under bile stress. Together, the multifactorial response helps BI_15697 excrete bile salts and maintain cellular integrity in response to bile stress. This study proposes a mechanism for the protection of BI_15697 against bile salt stress by MFGM, thereby providing a molecular basis for its application in incorporation of probiotics.http://www.sciencedirect.com/science/article/pii/S0022030223006082Bifidobacterium longum subsp. infantis ATCC 15697transcriptomic analysisbile salt stressmilk fat globule membrane |
| spellingShingle | Gongsheng Zhang Mingxue He Lihong Xiao Yuehua Jiao Jianchun Han Chun Li Michael J. Miller Lili Zhang Milk fat globule membrane protects Bifidobacterium longum ssp. infantis ATCC 15697 against bile stress by modifying global transcriptional responses Journal of Dairy Science Bifidobacterium longum subsp. infantis ATCC 15697 transcriptomic analysis bile salt stress milk fat globule membrane |
| title | Milk fat globule membrane protects Bifidobacterium longum ssp. infantis ATCC 15697 against bile stress by modifying global transcriptional responses |
| title_full | Milk fat globule membrane protects Bifidobacterium longum ssp. infantis ATCC 15697 against bile stress by modifying global transcriptional responses |
| title_fullStr | Milk fat globule membrane protects Bifidobacterium longum ssp. infantis ATCC 15697 against bile stress by modifying global transcriptional responses |
| title_full_unstemmed | Milk fat globule membrane protects Bifidobacterium longum ssp. infantis ATCC 15697 against bile stress by modifying global transcriptional responses |
| title_short | Milk fat globule membrane protects Bifidobacterium longum ssp. infantis ATCC 15697 against bile stress by modifying global transcriptional responses |
| title_sort | milk fat globule membrane protects bifidobacterium longum ssp infantis atcc 15697 against bile stress by modifying global transcriptional responses |
| topic | Bifidobacterium longum subsp. infantis ATCC 15697 transcriptomic analysis bile salt stress milk fat globule membrane |
| url | http://www.sciencedirect.com/science/article/pii/S0022030223006082 |
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