3D optogenetic control of arteriole diameter in vivo
Modulation of brain arteriole diameter is critical for maintaining cerebral blood pressure and controlling regional hyperemia during neural activity. However, studies of hemodynamic function in health and disease have lacked a method to control arteriole diameter independently with high spatiotempor...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2022-09-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/72802 |
| _version_ | 1811252635879079936 |
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| author | Philip J O'Herron David A Hartmann Kun Xie Prakash Kara Andy Y Shih |
| author_facet | Philip J O'Herron David A Hartmann Kun Xie Prakash Kara Andy Y Shih |
| author_sort | Philip J O'Herron |
| collection | DOAJ |
| description | Modulation of brain arteriole diameter is critical for maintaining cerebral blood pressure and controlling regional hyperemia during neural activity. However, studies of hemodynamic function in health and disease have lacked a method to control arteriole diameter independently with high spatiotemporal resolution. Here, we describe an all-optical approach to manipulate and monitor brain arteriole contractility in mice in three dimensions using combined in vivo two-photon optogenetics and imaging. The expression of the red-shifted excitatory opsin, ReaChR, in vascular smooth muscle cells enabled rapid and repeated vasoconstriction controlled by brief light pulses. Two-photon activation of ReaChR using a spatial light modulator produced highly localized constrictions when targeted to individual arterioles within the neocortex. We demonstrate the utility of this method for examining arteriole contractile dynamics and creating transient focal blood flow reductions. Additionally, we show that optogenetic constriction can be used to reshape vasodilatory responses to sensory stimulation, providing a valuable tool to dissociate blood flow changes from neural activity. |
| first_indexed | 2024-04-12T16:37:34Z |
| format | Article |
| id | doaj.art-5bd7287947e74030b7fa2e6f06917004 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T16:37:34Z |
| publishDate | 2022-09-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-5bd7287947e74030b7fa2e6f069170042022-12-22T03:24:56ZengeLife Sciences Publications LtdeLife2050-084X2022-09-011110.7554/eLife.728023D optogenetic control of arteriole diameter in vivoPhilip J O'Herron0https://orcid.org/0000-0002-8137-9432David A Hartmann1Kun Xie2Prakash Kara3https://orcid.org/0000-0002-4285-1634Andy Y Shih4Department of Physiology, Augusta University, Augusta, United States; Department of Neuroscience, Medical University of South Carolina, Charleston, United StatesDepartment of Neuroscience, Medical University of South Carolina, Charleston, United States; Department of Neurology & Neurological Sciences, Stanford University, Stanford, United StatesDepartment of Physiology, Augusta University, Augusta, United StatesDepartment of Neuroscience, Medical University of South Carolina, Charleston, United States; Department of Neuroscience, University of Minnesota, Minneapolis, United States; Center for Magnetic Resonance Research, University of Minnesota, Minneapolis, United StatesDepartment of Neuroscience, Medical University of South Carolina, Charleston, United States; Center for Developmental Biology and Regenerative Medicine, Seattle Children’s Research Institute, Seattle, United States; Department of Bioengineering, University of Washington, Seattle, United States; Department of Pediatrics, University of Washington, Seattle, United StatesModulation of brain arteriole diameter is critical for maintaining cerebral blood pressure and controlling regional hyperemia during neural activity. However, studies of hemodynamic function in health and disease have lacked a method to control arteriole diameter independently with high spatiotemporal resolution. Here, we describe an all-optical approach to manipulate and monitor brain arteriole contractility in mice in three dimensions using combined in vivo two-photon optogenetics and imaging. The expression of the red-shifted excitatory opsin, ReaChR, in vascular smooth muscle cells enabled rapid and repeated vasoconstriction controlled by brief light pulses. Two-photon activation of ReaChR using a spatial light modulator produced highly localized constrictions when targeted to individual arterioles within the neocortex. We demonstrate the utility of this method for examining arteriole contractile dynamics and creating transient focal blood flow reductions. Additionally, we show that optogenetic constriction can be used to reshape vasodilatory responses to sensory stimulation, providing a valuable tool to dissociate blood flow changes from neural activity.https://elifesciences.org/articles/72802optogeneticsvasculartwo-photonblood flow |
| spellingShingle | Philip J O'Herron David A Hartmann Kun Xie Prakash Kara Andy Y Shih 3D optogenetic control of arteriole diameter in vivo eLife optogenetics vascular two-photon blood flow |
| title | 3D optogenetic control of arteriole diameter in vivo |
| title_full | 3D optogenetic control of arteriole diameter in vivo |
| title_fullStr | 3D optogenetic control of arteriole diameter in vivo |
| title_full_unstemmed | 3D optogenetic control of arteriole diameter in vivo |
| title_short | 3D optogenetic control of arteriole diameter in vivo |
| title_sort | 3d optogenetic control of arteriole diameter in vivo |
| topic | optogenetics vascular two-photon blood flow |
| url | https://elifesciences.org/articles/72802 |
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