RAPD analysis of grapevine hybrids and cultivars
Utilization of the Randomly Amplified Polymorphic DNA (RAPD) technique as a molecular marker was tested to investigate the relationships between some representative grapevine cultivars and hybrids established at the Department of Genetics and Plant Breeding (CUB), to distinguish clones as well as to...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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University of Debrecen
2004-11-01
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| Series: | International Journal of Horticultural Science |
| Subjects: | |
| Online Access: | https://ojs.lib.unideb.hu/IJHS/article/view/515 |
| _version_ | 1818662367979896832 |
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| author | J. Halász J. Korbuly T. Deák Gy. D. Bisztray |
| author_facet | J. Halász J. Korbuly T. Deák Gy. D. Bisztray |
| author_sort | J. Halász |
| collection | DOAJ |
| description | Utilization of the Randomly Amplified Polymorphic DNA (RAPD) technique as a molecular marker was tested to investigate the relationships between some representative grapevine cultivars and hybrids established at the Department of Genetics and Plant Breeding (CUB), to distinguish clones as well as to characterize various hybrids between species or cultivars and their parents. Vitis vinifera cultivars were easily and successfully distinguished by the RAPD technique and they were grouped according to the traditional taxonomic classification. RAPD patterns of the examined Pinot gris clones proved to be completely identical. Number of generations was reflected by the value of genetic distance of the examined hybrids. Genetic identity of parents and their offsprings was influenced by the selection applied in the process of plant breeding. Parental phenotypic and morphologic characteristics showed high degree of segregation in hybrids, but RAPD analysis revealed that their genetic similarity is considerable. The three Vitis anntrensis clones were properly discriminated from every cultivar and hybrid of Vitis vinifera, i.e. hybrids are much closer to the cultivated grapevine than to V. anzurensis due to the phenotypic selection carried out during the life-cycle of one or two generations. |
| first_indexed | 2024-12-17T04:59:50Z |
| format | Article |
| id | doaj.art-5bd8279f77f340bba1722ae7a5fc89c7 |
| institution | Directory Open Access Journal |
| issn | 1585-0404 2676-931X |
| language | English |
| last_indexed | 2024-12-17T04:59:50Z |
| publishDate | 2004-11-01 |
| publisher | University of Debrecen |
| record_format | Article |
| series | International Journal of Horticultural Science |
| spelling | doaj.art-5bd8279f77f340bba1722ae7a5fc89c72022-12-21T22:02:35ZengUniversity of DebrecenInternational Journal of Horticultural Science1585-04042676-931X2004-11-0110410.31421/IJHS/10/4/515RAPD analysis of grapevine hybrids and cultivarsJ. Halász0J. Korbuly1T. Deák2Gy. D. Bisztray3Corvinus University of Budapest, Faculty of Horticultural Science, Department of Genetics and Plant Breeding, 1118 Budapest, Ménesi út 44/A., HungaryCorvinus University of Budapest, Faculty of Horticultural Science, Department of Genetics and Plant Breeding, 1118 Budapest, Ménesi út 44/A., HungaryCorvinus University of Budapest, Faculty of Horticultural Science, Department of Genetics and Plant Breeding, 1118 Budapest, Ménesi út 44/A., HungaryCorvinus University of Budapest, Faculty of Horticultural Science, Department of Genetics and Plant Breeding, 1118 Budapest, Ménesi út 44/A., HungaryUtilization of the Randomly Amplified Polymorphic DNA (RAPD) technique as a molecular marker was tested to investigate the relationships between some representative grapevine cultivars and hybrids established at the Department of Genetics and Plant Breeding (CUB), to distinguish clones as well as to characterize various hybrids between species or cultivars and their parents. Vitis vinifera cultivars were easily and successfully distinguished by the RAPD technique and they were grouped according to the traditional taxonomic classification. RAPD patterns of the examined Pinot gris clones proved to be completely identical. Number of generations was reflected by the value of genetic distance of the examined hybrids. Genetic identity of parents and their offsprings was influenced by the selection applied in the process of plant breeding. Parental phenotypic and morphologic characteristics showed high degree of segregation in hybrids, but RAPD analysis revealed that their genetic similarity is considerable. The three Vitis anntrensis clones were properly discriminated from every cultivar and hybrid of Vitis vinifera, i.e. hybrids are much closer to the cultivated grapevine than to V. anzurensis due to the phenotypic selection carried out during the life-cycle of one or two generations.https://ojs.lib.unideb.hu/IJHS/article/view/515cultivarsgrapevinehybridsRAPD PCRVitis viniferaVitis amurensis |
| spellingShingle | J. Halász J. Korbuly T. Deák Gy. D. Bisztray RAPD analysis of grapevine hybrids and cultivars International Journal of Horticultural Science cultivars grapevine hybrids RAPD PCR Vitis vinifera Vitis amurensis |
| title | RAPD analysis of grapevine hybrids and cultivars |
| title_full | RAPD analysis of grapevine hybrids and cultivars |
| title_fullStr | RAPD analysis of grapevine hybrids and cultivars |
| title_full_unstemmed | RAPD analysis of grapevine hybrids and cultivars |
| title_short | RAPD analysis of grapevine hybrids and cultivars |
| title_sort | rapd analysis of grapevine hybrids and cultivars |
| topic | cultivars grapevine hybrids RAPD PCR Vitis vinifera Vitis amurensis |
| url | https://ojs.lib.unideb.hu/IJHS/article/view/515 |
| work_keys_str_mv | AT jhalasz rapdanalysisofgrapevinehybridsandcultivars AT jkorbuly rapdanalysisofgrapevinehybridsandcultivars AT tdeak rapdanalysisofgrapevinehybridsandcultivars AT gydbisztray rapdanalysisofgrapevinehybridsandcultivars |