A comparison of three column agglutination tests for red blood cell alloantibody identification
Abstract Objective Commercial kits of column tests for pre-transfusion testing have progressively replaced conventional tube tests in most laboratories. Aim of this study was to compare three commercial test cell panels for the identification of irregular red blood cell (RBC) alloantibodies. Overall...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-03-01
|
| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13104-020-04974-x |
| _version_ | 1819228717054951424 |
|---|---|
| author | Siska Blomme Emilie De Maertelaere Eline Verhoye |
| author_facet | Siska Blomme Emilie De Maertelaere Eline Verhoye |
| author_sort | Siska Blomme |
| collection | DOAJ |
| description | Abstract Objective Commercial kits of column tests for pre-transfusion testing have progressively replaced conventional tube tests in most laboratories. Aim of this study was to compare three commercial test cell panels for the identification of irregular red blood cell (RBC) alloantibodies. Overall, 44 samples with a positive indirect antiglobulin test (IAT) by routine testing were used for comparison of following panels: Ortho RESOLVE® panelC (Ortho Clinical Diagnostics (OCD), Milan, Italy), ID-DiaPanel(-P) (Bio-Rad Laboratories, CA, USA) and Identisera Diana(P) (Grifols, Barcelona, Spain). Column agglutination techniques were used, with microtubes containing either microgel (Bio-Rad/Grifols) or glass bead microparticles (Ortho). Results Alloantibody identification was possible in 38 samples, of which identical identification was shown in 33 samples by all methods. The remaining samples showed differences between certain methods, with the gel card system being superior to the glass card system for analyzing stored samples Considering that not all samples were evaluated in all three methods, the concordance rate reached 100% between Bio-Rad and Grifols, 90.5% between Bio-Rad and OCD, 86.5% between OCD and Grifols and 90.5% between all methods. Although differences in sensitivities were seen for specific antibodies, the three methods showed comparable performance for the identification of RBC alloantibodies. |
| first_indexed | 2024-12-23T11:01:43Z |
| format | Article |
| id | doaj.art-5bd82f23b8654c04bffa80a49109368a |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-23T11:01:43Z |
| publishDate | 2020-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-5bd82f23b8654c04bffa80a49109368a2022-12-21T17:49:37ZengBMCBMC Research Notes1756-05002020-03-011311610.1186/s13104-020-04974-xA comparison of three column agglutination tests for red blood cell alloantibody identificationSiska Blomme0Emilie De Maertelaere1Eline Verhoye2Clinical Department of Laboratory Medicine, University HospitalsClinical Department of Laboratory Medicine, University HospitalsClinical Departments of Laboratory Medicine, AZ Delta HospitalAbstract Objective Commercial kits of column tests for pre-transfusion testing have progressively replaced conventional tube tests in most laboratories. Aim of this study was to compare three commercial test cell panels for the identification of irregular red blood cell (RBC) alloantibodies. Overall, 44 samples with a positive indirect antiglobulin test (IAT) by routine testing were used for comparison of following panels: Ortho RESOLVE® panelC (Ortho Clinical Diagnostics (OCD), Milan, Italy), ID-DiaPanel(-P) (Bio-Rad Laboratories, CA, USA) and Identisera Diana(P) (Grifols, Barcelona, Spain). Column agglutination techniques were used, with microtubes containing either microgel (Bio-Rad/Grifols) or glass bead microparticles (Ortho). Results Alloantibody identification was possible in 38 samples, of which identical identification was shown in 33 samples by all methods. The remaining samples showed differences between certain methods, with the gel card system being superior to the glass card system for analyzing stored samples Considering that not all samples were evaluated in all three methods, the concordance rate reached 100% between Bio-Rad and Grifols, 90.5% between Bio-Rad and OCD, 86.5% between OCD and Grifols and 90.5% between all methods. Although differences in sensitivities were seen for specific antibodies, the three methods showed comparable performance for the identification of RBC alloantibodies.http://link.springer.com/article/10.1186/s13104-020-04974-xColumn agglutination techniqueRed blood cell antibodiesAlloantibody identificationTransfusion medicinePre-transfusion testing |
| spellingShingle | Siska Blomme Emilie De Maertelaere Eline Verhoye A comparison of three column agglutination tests for red blood cell alloantibody identification BMC Research Notes Column agglutination technique Red blood cell antibodies Alloantibody identification Transfusion medicine Pre-transfusion testing |
| title | A comparison of three column agglutination tests for red blood cell alloantibody identification |
| title_full | A comparison of three column agglutination tests for red blood cell alloantibody identification |
| title_fullStr | A comparison of three column agglutination tests for red blood cell alloantibody identification |
| title_full_unstemmed | A comparison of three column agglutination tests for red blood cell alloantibody identification |
| title_short | A comparison of three column agglutination tests for red blood cell alloantibody identification |
| title_sort | comparison of three column agglutination tests for red blood cell alloantibody identification |
| topic | Column agglutination technique Red blood cell antibodies Alloantibody identification Transfusion medicine Pre-transfusion testing |
| url | http://link.springer.com/article/10.1186/s13104-020-04974-x |
| work_keys_str_mv | AT siskablomme acomparisonofthreecolumnagglutinationtestsforredbloodcellalloantibodyidentification AT emiliedemaertelaere acomparisonofthreecolumnagglutinationtestsforredbloodcellalloantibodyidentification AT elineverhoye acomparisonofthreecolumnagglutinationtestsforredbloodcellalloantibodyidentification AT siskablomme comparisonofthreecolumnagglutinationtestsforredbloodcellalloantibodyidentification AT emiliedemaertelaere comparisonofthreecolumnagglutinationtestsforredbloodcellalloantibodyidentification AT elineverhoye comparisonofthreecolumnagglutinationtestsforredbloodcellalloantibodyidentification |