In vitro Anti-viral Activity of Psoraleae Semen Water Extract Against Influenza A Viruses
Influenza causes respiratory infections and poses health risks to humans and animals; its effects are complicated by increasing resistance to existing anti-influenza viral agents. Therefore, novel therapeutic approaches against influenza virus infection are required. Psoraleae semen has been widely...
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| Format: | Article |
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Frontiers Media S.A.
2016-11-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fphar.2016.00460/full |
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| author | Jang-Gi Choi Young-Hee Jin Ji-Hye Kim Tae Woo Oh Nam-Hui Yim Won-Kyung Cho Jin Yeul Ma |
| author_facet | Jang-Gi Choi Young-Hee Jin Ji-Hye Kim Tae Woo Oh Nam-Hui Yim Won-Kyung Cho Jin Yeul Ma |
| author_sort | Jang-Gi Choi |
| collection | DOAJ |
| description | Influenza causes respiratory infections and poses health risks to humans and animals; its effects are complicated by increasing resistance to existing anti-influenza viral agents. Therefore, novel therapeutic approaches against influenza virus infection are required. Psoraleae semen has been widely used in traditional medicine in Korea, Taiwan, China, and Japan for treating and preventing various diseases. In this study, we examined the anti-viral activities and mechanism of action of the water extract of Psoraleae semen (WPS) using RAW 264.7 and MDCK cells. We found that pre- and post-treatment with 100 µg/mL WPS markedly inhibited influenza A virus replication as assessed using a green fluorescent protein reporter virus, reduced viral protein expression (NS-1, PA, HA, PB-1, M1, and M2), and inhibited NA and HA activities. Mechanism studies revealed that WPS induced type I interferon cytokine secretion and subsequent stimulation of an anti-viral state in RAW 264.7 cells. Further, WPS exerted inhibitory effects on neuraminidase in influenza virus strains H1N1 and H3N2. Meanwhile, WPS exhibited inhibitory effects on hemagglutination in H3N2 but not in H1N1. Based on these results, WPS serves as an immunomodulator and inhibitor of influenza hemagglutinin and neuraminidase. Our results suggest that WPS is a promising source of novel anti-influenza drug candidates. |
| first_indexed | 2024-12-18T08:24:13Z |
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| id | doaj.art-5bda98ec34f1403aac8971d6ba94a4f9 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-18T08:24:13Z |
| publishDate | 2016-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-5bda98ec34f1403aac8971d6ba94a4f92022-12-21T21:14:40ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122016-11-01710.3389/fphar.2016.00460226202In vitro Anti-viral Activity of Psoraleae Semen Water Extract Against Influenza A VirusesJang-Gi Choi0Young-Hee Jin1Ji-Hye Kim2Tae Woo Oh3Nam-Hui Yim4Won-Kyung Cho5Jin Yeul Ma6Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM)Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM)Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM)Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM)Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM)Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM)Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM)Influenza causes respiratory infections and poses health risks to humans and animals; its effects are complicated by increasing resistance to existing anti-influenza viral agents. Therefore, novel therapeutic approaches against influenza virus infection are required. Psoraleae semen has been widely used in traditional medicine in Korea, Taiwan, China, and Japan for treating and preventing various diseases. In this study, we examined the anti-viral activities and mechanism of action of the water extract of Psoraleae semen (WPS) using RAW 264.7 and MDCK cells. We found that pre- and post-treatment with 100 µg/mL WPS markedly inhibited influenza A virus replication as assessed using a green fluorescent protein reporter virus, reduced viral protein expression (NS-1, PA, HA, PB-1, M1, and M2), and inhibited NA and HA activities. Mechanism studies revealed that WPS induced type I interferon cytokine secretion and subsequent stimulation of an anti-viral state in RAW 264.7 cells. Further, WPS exerted inhibitory effects on neuraminidase in influenza virus strains H1N1 and H3N2. Meanwhile, WPS exhibited inhibitory effects on hemagglutination in H3N2 but not in H1N1. Based on these results, WPS serves as an immunomodulator and inhibitor of influenza hemagglutinin and neuraminidase. Our results suggest that WPS is a promising source of novel anti-influenza drug candidates.http://journal.frontiersin.org/Journal/10.3389/fphar.2016.00460/fullInfluenza A virusH1N1H3N2Anti-viral activityPsoraleae semen water extract |
| spellingShingle | Jang-Gi Choi Young-Hee Jin Ji-Hye Kim Tae Woo Oh Nam-Hui Yim Won-Kyung Cho Jin Yeul Ma In vitro Anti-viral Activity of Psoraleae Semen Water Extract Against Influenza A Viruses Frontiers in Pharmacology Influenza A virus H1N1 H3N2 Anti-viral activity Psoraleae semen water extract |
| title | In vitro Anti-viral Activity of Psoraleae Semen Water Extract Against Influenza A Viruses |
| title_full | In vitro Anti-viral Activity of Psoraleae Semen Water Extract Against Influenza A Viruses |
| title_fullStr | In vitro Anti-viral Activity of Psoraleae Semen Water Extract Against Influenza A Viruses |
| title_full_unstemmed | In vitro Anti-viral Activity of Psoraleae Semen Water Extract Against Influenza A Viruses |
| title_short | In vitro Anti-viral Activity of Psoraleae Semen Water Extract Against Influenza A Viruses |
| title_sort | in vitro anti viral activity of psoraleae semen water extract against influenza a viruses |
| topic | Influenza A virus H1N1 H3N2 Anti-viral activity Psoraleae semen water extract |
| url | http://journal.frontiersin.org/Journal/10.3389/fphar.2016.00460/full |
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