Cytogenetic effects of combination of tridecactide and met-enkephalin on lymphocytes of patients with multiple sclerosis
Introduction: The met-enkephalin (1-5 Adrenorphin) and tridecactide (alpha-corticotropin 1-13) combination is used in the multiple sclerosis (MS) immuno-modulatory treatment. A testing of cytogenetic effects of met-enkephalin resulted in reductions of lymphocytic aberrations in the in the lymphocyt...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Sarajevo
2015-04-01
|
| Series: | Journal of Health Sciences |
| Subjects: | |
| Online Access: | https://www.jhsci.ba/ojs/index.php/jhsci/article/view/379 |
| _version_ | 1818208334905344000 |
|---|---|
| author | Maida Rakanovic-Todic Slavka Ibrulj Lejla Burnazovic-Ristic Amra Catovic Izeta Aganovic-Musinovic Jasna Kusturica |
| author_facet | Maida Rakanovic-Todic Slavka Ibrulj Lejla Burnazovic-Ristic Amra Catovic Izeta Aganovic-Musinovic Jasna Kusturica |
| author_sort | Maida Rakanovic-Todic |
| collection | DOAJ |
| description | Introduction: The met-enkephalin (1-5 Adrenorphin) and tridecactide (alpha-corticotropin 1-13) combination is used in the multiple sclerosis (MS) immuno-modulatory treatment. A testing of cytogenetic effects of met-enkephalin resulted in reductions of lymphocytic aberrations in the in the lymphocytes of the peripheral blood (PBL) of patients with immune-mediated diseases. The aim of this research is to evaluate the in vitro effects of the combination of the met-enkephalin and tridecactide on the number and the type of chromosome aberrations in PBL of the MS patients.
Methods: We used blood samples from seven female patients with the diagnosis multiple sclerosis based on a McDonald Diagnostic Criteria. The tested combination, met-enkephalin and alpha-ACTH 1-13 was added at three different concentrations and constant volume.
Results: Results showed that the combination of tested substances did not reduce the number of structural aberrations, although the treatment did not result in severe aberrations such as ring, fragmented, and dicentric chromosomes. Furthermore, it elicited an increase in the number of numerical aberrations and aneuploidy after the treatment with the test combo.
Conclusion: As the changes in ploidy significantly change the DNA as well as the biochemical cell phenotype, we concluded that more research in this field should be conducted, including both toxicological as well as the pharmacodynamic considerations. |
| first_indexed | 2024-12-12T04:43:10Z |
| format | Article |
| id | doaj.art-5bdee8390681494f8f79a26f478a35f2 |
| institution | Directory Open Access Journal |
| issn | 2232-7576 1986-8049 |
| language | English |
| last_indexed | 2024-12-12T04:43:10Z |
| publishDate | 2015-04-01 |
| publisher | University of Sarajevo |
| record_format | Article |
| series | Journal of Health Sciences |
| spelling | doaj.art-5bdee8390681494f8f79a26f478a35f22022-12-22T00:37:43ZengUniversity of SarajevoJournal of Health Sciences2232-75761986-80492015-04-015110.17532/jhsci.2015.237159Cytogenetic effects of combination of tridecactide and met-enkephalin on lymphocytes of patients with multiple sclerosisMaida Rakanovic-Todic0Slavka Ibrulj1Lejla Burnazovic-Ristic2Amra Catovic3Izeta Aganovic-Musinovic4Jasna Kusturica5Institute/Cathedra of Pharmacology, Clinical Pharmacology and Toxicology, Medical Faculty, University of SarajevoCenter for Cytogenetics and Molecuar Medicine Medical Faculty of University of SarajevoInstitute/Cathedra of Pharmacology, Clinical Pharmacology and Toxicology, Medical Faculty, University of SarajevoCenter for Cytogenetics and Molecuar Medicine Medical Faculty of University of SarajevoCenter for Cytogenetics and Molecuar Medicine Medical Faculty of University of SarajevoInstitute/Cathedra of Pharmacology, Clinical Pharmacology and Toxicology, Medical Faculty, University of SarajevoIntroduction: The met-enkephalin (1-5 Adrenorphin) and tridecactide (alpha-corticotropin 1-13) combination is used in the multiple sclerosis (MS) immuno-modulatory treatment. A testing of cytogenetic effects of met-enkephalin resulted in reductions of lymphocytic aberrations in the in the lymphocytes of the peripheral blood (PBL) of patients with immune-mediated diseases. The aim of this research is to evaluate the in vitro effects of the combination of the met-enkephalin and tridecactide on the number and the type of chromosome aberrations in PBL of the MS patients. Methods: We used blood samples from seven female patients with the diagnosis multiple sclerosis based on a McDonald Diagnostic Criteria. The tested combination, met-enkephalin and alpha-ACTH 1-13 was added at three different concentrations and constant volume. Results: Results showed that the combination of tested substances did not reduce the number of structural aberrations, although the treatment did not result in severe aberrations such as ring, fragmented, and dicentric chromosomes. Furthermore, it elicited an increase in the number of numerical aberrations and aneuploidy after the treatment with the test combo. Conclusion: As the changes in ploidy significantly change the DNA as well as the biochemical cell phenotype, we concluded that more research in this field should be conducted, including both toxicological as well as the pharmacodynamic considerations.https://www.jhsci.ba/ojs/index.php/jhsci/article/view/379alpha-corticotropin 1-13tridecactidemet-enkephalinchromosome aberrationMultiple Sclerosis-enkephalin |
| spellingShingle | Maida Rakanovic-Todic Slavka Ibrulj Lejla Burnazovic-Ristic Amra Catovic Izeta Aganovic-Musinovic Jasna Kusturica Cytogenetic effects of combination of tridecactide and met-enkephalin on lymphocytes of patients with multiple sclerosis Journal of Health Sciences alpha-corticotropin 1-13 tridecactide met-enkephalin chromosome aberration Multiple Sclerosis -enkephalin |
| title | Cytogenetic effects of combination of tridecactide and met-enkephalin on lymphocytes of patients with multiple sclerosis |
| title_full | Cytogenetic effects of combination of tridecactide and met-enkephalin on lymphocytes of patients with multiple sclerosis |
| title_fullStr | Cytogenetic effects of combination of tridecactide and met-enkephalin on lymphocytes of patients with multiple sclerosis |
| title_full_unstemmed | Cytogenetic effects of combination of tridecactide and met-enkephalin on lymphocytes of patients with multiple sclerosis |
| title_short | Cytogenetic effects of combination of tridecactide and met-enkephalin on lymphocytes of patients with multiple sclerosis |
| title_sort | cytogenetic effects of combination of tridecactide and met enkephalin on lymphocytes of patients with multiple sclerosis |
| topic | alpha-corticotropin 1-13 tridecactide met-enkephalin chromosome aberration Multiple Sclerosis -enkephalin |
| url | https://www.jhsci.ba/ojs/index.php/jhsci/article/view/379 |
| work_keys_str_mv | AT maidarakanovictodic cytogeneticeffectsofcombinationoftridecactideandmetenkephalinonlymphocytesofpatientswithmultiplesclerosis AT slavkaibrulj cytogeneticeffectsofcombinationoftridecactideandmetenkephalinonlymphocytesofpatientswithmultiplesclerosis AT lejlaburnazovicristic cytogeneticeffectsofcombinationoftridecactideandmetenkephalinonlymphocytesofpatientswithmultiplesclerosis AT amracatovic cytogeneticeffectsofcombinationoftridecactideandmetenkephalinonlymphocytesofpatientswithmultiplesclerosis AT izetaaganovicmusinovic cytogeneticeffectsofcombinationoftridecactideandmetenkephalinonlymphocytesofpatientswithmultiplesclerosis AT jasnakusturica cytogeneticeffectsofcombinationoftridecactideandmetenkephalinonlymphocytesofpatientswithmultiplesclerosis |