Acetic anhydride–trifluoroacetic acid acetolysis for the estimation of glycerol in phosphatidyl choline by gas–liquid chromatography
Low glycerol recoveries are obtained when acetic anhydride–acetic acid acetolysis is used to dephosphorylate lecithin prior to glycerol (and fatty acid) analysis by saponification–acetylation and gas–liquid chromatography.Quantitative glycerol recoveries are obtained for both phosphatidyl choline an...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1965-04-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520390039 |
| _version_ | 1824023211801575424 |
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| author | K.S. Holla David G. Cornwell |
| author_facet | K.S. Holla David G. Cornwell |
| author_sort | K.S. Holla |
| collection | DOAJ |
| description | Low glycerol recoveries are obtained when acetic anhydride–acetic acid acetolysis is used to dephosphorylate lecithin prior to glycerol (and fatty acid) analysis by saponification–acetylation and gas–liquid chromatography.Quantitative glycerol recoveries are obtained for both phosphatidyl choline and phosphatidyl ethanolamine when a modified acetolysis using acetic anhydride–trifluoroacetic acid is employed. Modified acetolysis is limited to the analysis of glycerol alone since unsaturated fatty acids are destroyed. |
| first_indexed | 2024-12-19T09:31:58Z |
| format | Article |
| id | doaj.art-5be0f3377a134cc78c0e37075c4856d8 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-19T09:31:58Z |
| publishDate | 1965-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-5be0f3377a134cc78c0e37075c4856d82022-12-21T20:27:36ZengElsevierJournal of Lipid Research0022-22751965-04-0162322324Acetic anhydride–trifluoroacetic acid acetolysis for the estimation of glycerol in phosphatidyl choline by gas–liquid chromatographyK.S. Holla0David G. Cornwell1Department of Physiological Chemistry, The Ohio State University, Columbus, OhioDepartment of Physiological Chemistry, The Ohio State University, Columbus, OhioLow glycerol recoveries are obtained when acetic anhydride–acetic acid acetolysis is used to dephosphorylate lecithin prior to glycerol (and fatty acid) analysis by saponification–acetylation and gas–liquid chromatography.Quantitative glycerol recoveries are obtained for both phosphatidyl choline and phosphatidyl ethanolamine when a modified acetolysis using acetic anhydride–trifluoroacetic acid is employed. Modified acetolysis is limited to the analysis of glycerol alone since unsaturated fatty acids are destroyed.http://www.sciencedirect.com/science/article/pii/S0022227520390039glycerol estimationphosphatidyl choline phosphatidyl ethanolamineacetolysistrifluoroacetic acidglyceryl triacetategas–liquid chromatography |
| spellingShingle | K.S. Holla David G. Cornwell Acetic anhydride–trifluoroacetic acid acetolysis for the estimation of glycerol in phosphatidyl choline by gas–liquid chromatography Journal of Lipid Research glycerol estimation phosphatidyl choline phosphatidyl ethanolamine acetolysis trifluoroacetic acid glyceryl triacetate gas–liquid chromatography |
| title | Acetic anhydride–trifluoroacetic acid acetolysis for the estimation of glycerol in phosphatidyl choline by gas–liquid chromatography |
| title_full | Acetic anhydride–trifluoroacetic acid acetolysis for the estimation of glycerol in phosphatidyl choline by gas–liquid chromatography |
| title_fullStr | Acetic anhydride–trifluoroacetic acid acetolysis for the estimation of glycerol in phosphatidyl choline by gas–liquid chromatography |
| title_full_unstemmed | Acetic anhydride–trifluoroacetic acid acetolysis for the estimation of glycerol in phosphatidyl choline by gas–liquid chromatography |
| title_short | Acetic anhydride–trifluoroacetic acid acetolysis for the estimation of glycerol in phosphatidyl choline by gas–liquid chromatography |
| title_sort | acetic anhydride trifluoroacetic acid acetolysis for the estimation of glycerol in phosphatidyl choline by gas liquid chromatography |
| topic | glycerol estimation phosphatidyl choline phosphatidyl ethanolamine acetolysis trifluoroacetic acid glyceryl triacetate gas–liquid chromatography |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520390039 |
| work_keys_str_mv | AT ksholla aceticanhydridetrifluoroaceticacidacetolysisfortheestimationofglycerolinphosphatidylcholinebygasliquidchromatography AT davidgcornwell aceticanhydridetrifluoroaceticacidacetolysisfortheestimationofglycerolinphosphatidylcholinebygasliquidchromatography |