Quantitative field testing Rotylenchulus reniformis DNA from metagenomic samples isolated directly from soil.
A quantitative PCR procedure targeting the β-tubulin gene determined the number of Rotylenchulus reniformis Linford & Oliveira 1940 in metagenomic DNA samples isolated from soil. Of note, this outcome was in the presence of other soil-dwelling plant parasitic nematodes including its sister genus...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3241691?pdf=render |
| _version_ | 1818841252451319808 |
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| author | Kurt Showmaker Gary W Lawrence Shien Lu Clarissa Balbalian Vincent P Klink |
| author_facet | Kurt Showmaker Gary W Lawrence Shien Lu Clarissa Balbalian Vincent P Klink |
| author_sort | Kurt Showmaker |
| collection | DOAJ |
| description | A quantitative PCR procedure targeting the β-tubulin gene determined the number of Rotylenchulus reniformis Linford & Oliveira 1940 in metagenomic DNA samples isolated from soil. Of note, this outcome was in the presence of other soil-dwelling plant parasitic nematodes including its sister genus Helicotylenchus Steiner, 1945. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from soil. |
| first_indexed | 2024-12-19T04:23:08Z |
| format | Article |
| id | doaj.art-5be3111a7c6a4db0b6c157a750fac212 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T04:23:08Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5be3111a7c6a4db0b6c157a750fac2122022-12-21T20:36:06ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01612e2895410.1371/journal.pone.0028954Quantitative field testing Rotylenchulus reniformis DNA from metagenomic samples isolated directly from soil.Kurt ShowmakerGary W LawrenceShien LuClarissa BalbalianVincent P KlinkA quantitative PCR procedure targeting the β-tubulin gene determined the number of Rotylenchulus reniformis Linford & Oliveira 1940 in metagenomic DNA samples isolated from soil. Of note, this outcome was in the presence of other soil-dwelling plant parasitic nematodes including its sister genus Helicotylenchus Steiner, 1945. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from soil.http://europepmc.org/articles/PMC3241691?pdf=render |
| spellingShingle | Kurt Showmaker Gary W Lawrence Shien Lu Clarissa Balbalian Vincent P Klink Quantitative field testing Rotylenchulus reniformis DNA from metagenomic samples isolated directly from soil. PLoS ONE |
| title | Quantitative field testing Rotylenchulus reniformis DNA from metagenomic samples isolated directly from soil. |
| title_full | Quantitative field testing Rotylenchulus reniformis DNA from metagenomic samples isolated directly from soil. |
| title_fullStr | Quantitative field testing Rotylenchulus reniformis DNA from metagenomic samples isolated directly from soil. |
| title_full_unstemmed | Quantitative field testing Rotylenchulus reniformis DNA from metagenomic samples isolated directly from soil. |
| title_short | Quantitative field testing Rotylenchulus reniformis DNA from metagenomic samples isolated directly from soil. |
| title_sort | quantitative field testing rotylenchulus reniformis dna from metagenomic samples isolated directly from soil |
| url | http://europepmc.org/articles/PMC3241691?pdf=render |
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