Comparison of Optical Imaging Techniques to Quantitatively Assess the Perfusion of the Gastric Conduit during Oesophagectomy

In this study, four optical techniques—Optical Coherence Tomography, Sidestream Darkfield Microscopy, Laser Speckle Contrast Imaging, and Fluorescence Angiography (FA)—were compared on performing an intraoperative quantitative perfusion assessment of the gastric conduit during oesophagectomy. We hyp...

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Main Authors: Maxime D. Slooter, Sanne M. A. Jansen, Paul R. Bloemen, Richard M. van den Elzen, Leah S. Wilk, Ton G. van Leeuwen, Mark I. van Berge Henegouwen, Daniel M. de Bruin, Suzanne S. Gisbertz
Format: Article
Language:English
Published: MDPI AG 2020-08-01
Series:Applied Sciences
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Online Access:https://www.mdpi.com/2076-3417/10/16/5522
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author Maxime D. Slooter
Sanne M. A. Jansen
Paul R. Bloemen
Richard M. van den Elzen
Leah S. Wilk
Ton G. van Leeuwen
Mark I. van Berge Henegouwen
Daniel M. de Bruin
Suzanne S. Gisbertz
author_facet Maxime D. Slooter
Sanne M. A. Jansen
Paul R. Bloemen
Richard M. van den Elzen
Leah S. Wilk
Ton G. van Leeuwen
Mark I. van Berge Henegouwen
Daniel M. de Bruin
Suzanne S. Gisbertz
author_sort Maxime D. Slooter
collection DOAJ
description In this study, four optical techniques—Optical Coherence Tomography, Sidestream Darkfield Microscopy, Laser Speckle Contrast Imaging, and Fluorescence Angiography (FA)—were compared on performing an intraoperative quantitative perfusion assessment of the gastric conduit during oesophagectomy. We hypothesised that the quantitative parameters show decreased perfusion towards the fundus in the gastric conduit and in patients with anastomotic leakage. In a prospective study in patients undergoing oesophagectomy with gastric conduit reconstruction, measurements were taken with all four optical techniques at four locations from the base towards the fundus in the gastric conduit (Loc1, Loc2, Loc3, Loc4). The primary outcome included 14 quantitative parameters and the anastomotic leakage rate. Imaging was performed in 22 patients during oesophagectomy. Ten out of 14 quantitative parameters significantly indicated a reduced perfusion towards the fundus of the gastric conduit. Anastomotic leakage occurred in 4/22 patients (18.4%). At Loc4, the FA quantitative values for “T<sub>1/2</sub>” and “mean slope” differed between patients with and without anastomotic leakage (<i>p</i> = 0.025 and <i>p</i> = 0.041, respectively). A quantitative perfusion assessment during oesophagectomy is feasible using optical imaging techniques, of which FA is the most promising for future research.
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spelling doaj.art-5be7ffb00a8b429dab402cb56822fe182023-11-20T09:39:02ZengMDPI AGApplied Sciences2076-34172020-08-011016552210.3390/app10165522Comparison of Optical Imaging Techniques to Quantitatively Assess the Perfusion of the Gastric Conduit during OesophagectomyMaxime D. Slooter0Sanne M. A. Jansen1Paul R. Bloemen2Richard M. van den Elzen3Leah S. Wilk4Ton G. van Leeuwen5Mark I. van Berge Henegouwen6Daniel M. de Bruin7Suzanne S. Gisbertz8Department of Surgery, Cancer Center Amsterdam, Amsterdam UMC, University of Amsterdam, 1105 AZ Amsterdam, The NetherlandsDepartment of Biomedical Engineering & Physics, Cancer Center Amsterdam, Amsterdam Cardiovascular Sciences, Amsterdam UMC, University of Amsterdam, 1105 AZ Amsterdam, The NetherlandsDepartment of Biomedical Engineering & Physics, Cancer Center Amsterdam, Amsterdam Cardiovascular Sciences, Amsterdam UMC, University of Amsterdam, 1105 AZ Amsterdam, The NetherlandsDepartment of Biomedical Engineering & Physics, Cancer Center Amsterdam, Amsterdam Cardiovascular Sciences, Amsterdam UMC, University of Amsterdam, 1105 AZ Amsterdam, The NetherlandsDepartment of Biomedical Engineering & Physics, Cancer Center Amsterdam, Amsterdam Cardiovascular Sciences, Amsterdam UMC, University of Amsterdam, 1105 AZ Amsterdam, The NetherlandsDepartment of Biomedical Engineering & Physics, Cancer Center Amsterdam, Amsterdam Cardiovascular Sciences, Amsterdam UMC, University of Amsterdam, 1105 AZ Amsterdam, The NetherlandsDepartment of Surgery, Cancer Center Amsterdam, Amsterdam UMC, University of Amsterdam, 1105 AZ Amsterdam, The NetherlandsDepartment of Biomedical Engineering & Physics, Cancer Center Amsterdam, Amsterdam Cardiovascular Sciences, Amsterdam UMC, University of Amsterdam, 1105 AZ Amsterdam, The NetherlandsDepartment of Surgery, Cancer Center Amsterdam, Amsterdam UMC, University of Amsterdam, 1105 AZ Amsterdam, The NetherlandsIn this study, four optical techniques—Optical Coherence Tomography, Sidestream Darkfield Microscopy, Laser Speckle Contrast Imaging, and Fluorescence Angiography (FA)—were compared on performing an intraoperative quantitative perfusion assessment of the gastric conduit during oesophagectomy. We hypothesised that the quantitative parameters show decreased perfusion towards the fundus in the gastric conduit and in patients with anastomotic leakage. In a prospective study in patients undergoing oesophagectomy with gastric conduit reconstruction, measurements were taken with all four optical techniques at four locations from the base towards the fundus in the gastric conduit (Loc1, Loc2, Loc3, Loc4). The primary outcome included 14 quantitative parameters and the anastomotic leakage rate. Imaging was performed in 22 patients during oesophagectomy. Ten out of 14 quantitative parameters significantly indicated a reduced perfusion towards the fundus of the gastric conduit. Anastomotic leakage occurred in 4/22 patients (18.4%). At Loc4, the FA quantitative values for “T<sub>1/2</sub>” and “mean slope” differed between patients with and without anastomotic leakage (<i>p</i> = 0.025 and <i>p</i> = 0.041, respectively). A quantitative perfusion assessment during oesophagectomy is feasible using optical imaging techniques, of which FA is the most promising for future research.https://www.mdpi.com/2076-3417/10/16/5522optical imagingfluorescence imagingfluorescence angiographyindocyanine green (ICG)optical coherence tomography (OCT)laser speckle contrast imaging (LSCI)
spellingShingle Maxime D. Slooter
Sanne M. A. Jansen
Paul R. Bloemen
Richard M. van den Elzen
Leah S. Wilk
Ton G. van Leeuwen
Mark I. van Berge Henegouwen
Daniel M. de Bruin
Suzanne S. Gisbertz
Comparison of Optical Imaging Techniques to Quantitatively Assess the Perfusion of the Gastric Conduit during Oesophagectomy
Applied Sciences
optical imaging
fluorescence imaging
fluorescence angiography
indocyanine green (ICG)
optical coherence tomography (OCT)
laser speckle contrast imaging (LSCI)
title Comparison of Optical Imaging Techniques to Quantitatively Assess the Perfusion of the Gastric Conduit during Oesophagectomy
title_full Comparison of Optical Imaging Techniques to Quantitatively Assess the Perfusion of the Gastric Conduit during Oesophagectomy
title_fullStr Comparison of Optical Imaging Techniques to Quantitatively Assess the Perfusion of the Gastric Conduit during Oesophagectomy
title_full_unstemmed Comparison of Optical Imaging Techniques to Quantitatively Assess the Perfusion of the Gastric Conduit during Oesophagectomy
title_short Comparison of Optical Imaging Techniques to Quantitatively Assess the Perfusion of the Gastric Conduit during Oesophagectomy
title_sort comparison of optical imaging techniques to quantitatively assess the perfusion of the gastric conduit during oesophagectomy
topic optical imaging
fluorescence imaging
fluorescence angiography
indocyanine green (ICG)
optical coherence tomography (OCT)
laser speckle contrast imaging (LSCI)
url https://www.mdpi.com/2076-3417/10/16/5522
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