Transcription factor 7-like 2 as type-2 diabetes mellitus diagnostic marker in ethnic Minangkabau
BACKGROUND The prevalence of type-2 diabetes mellitus (T2DM) has been increasing rapidly in developed and developing countries. Many new loci associated with T2DM have been uncovered by genetic and genome-wide association studies, eg. the transcription factor 7-like 2 (TCF7L2) gene variant rs789534...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Medicine Trisakti University
2014-12-01
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| Series: | Universa Medicina |
| Subjects: | |
| Online Access: | http://www.univmed.org/wp-content/uploads/2015/02/Syamsurizal.pdf |
| _version_ | 1819071276563562496 |
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| author | Syamsurizal Yanwirasti Asman Manaf Jamsari ML Edy Parwanto Arif Sardi |
| author_facet | Syamsurizal Yanwirasti Asman Manaf Jamsari ML Edy Parwanto Arif Sardi |
| author_sort | Syamsurizal |
| collection | DOAJ |
| description | BACKGROUND
The prevalence of type-2 diabetes mellitus (T2DM) has been increasing rapidly in developed and developing countries. Many new loci associated with T2DM have been uncovered by genetic and genome-wide association studies, eg. the transcription factor 7-like 2 (TCF7L2) gene variant rs7895340 has been associated with type-2 diabetes mellitus. The aim of this research was to find an association between polymorphisms of TCF7L2 gene variant rs7895340 with T2DM in ethnic Minangkabau.
METHODS
This was a case-control study using a consecutive sampling technique among ethnic Minangkabau patients who came for treatment to the Metabolic Endocrinology Polyclinic at Dr. M. Jamil Hospital. Analysis was performed on 132 subjects (66 T2DM patients and 66 controls). DNA was isolated from venous blood samples and amplified using the amplification refractory mutation system - polymerase chain reaction (ARMS-PCR) method to detect single nucleotide polymorphisms (SNPs) of TCF7L2 gene variant rs7895340. To determine the significance of association of SNPs of rs7895340 with T2DM in ethnic Minangkabau, data analysis was performed using chi-square test.
RESULTS
The ARMS - PCR for TCF7L2 gene variant rs7895340 found polymorphisms in 28 (42.42%) subjects with type 2 diabetes mellitus and in 15 (22.72%) non-diabetic subjects.There was a significant association between rs7895340 SNPs with the probability of T2DM in ethnic Minangkabau (OR=2.505, p=0.026).
CONCLUSIONS
Single nucleotide polymorphism rs7895340 of TCF7L2 gene can be used as genetic marker of type-2 diabetes mellitus in ethnic Minangkabau. |
| first_indexed | 2024-12-21T17:19:16Z |
| format | Article |
| id | doaj.art-5bea37daf2ff4643a4f797fd283c9593 |
| institution | Directory Open Access Journal |
| issn | 1907-3062 |
| language | English |
| last_indexed | 2024-12-21T17:19:16Z |
| publishDate | 2014-12-01 |
| publisher | Faculty of Medicine Trisakti University |
| record_format | Article |
| series | Universa Medicina |
| spelling | doaj.art-5bea37daf2ff4643a4f797fd283c95932022-12-21T18:56:12ZengFaculty of Medicine Trisakti UniversityUniversa Medicina1907-30622014-12-01333205212Transcription factor 7-like 2 as type-2 diabetes mellitus diagnostic marker in ethnic MinangkabauSyamsurizal0Yanwirasti1Asman Manaf2Jamsari3ML Edy Parwanto4Arif Sardi5Faculty of Mathematics and Natural Sciences, Padang State University Faculty of Medicine, Andalas University Faculty of Medicine, Andalas University Faculty of Agriculture, Andalas UniversityFaculty of Medicine, Trisakti UniversityFaculty of Science and Technology, Ar-Raniry Islamic State University, Banda AcehBACKGROUND The prevalence of type-2 diabetes mellitus (T2DM) has been increasing rapidly in developed and developing countries. Many new loci associated with T2DM have been uncovered by genetic and genome-wide association studies, eg. the transcription factor 7-like 2 (TCF7L2) gene variant rs7895340 has been associated with type-2 diabetes mellitus. The aim of this research was to find an association between polymorphisms of TCF7L2 gene variant rs7895340 with T2DM in ethnic Minangkabau. METHODS This was a case-control study using a consecutive sampling technique among ethnic Minangkabau patients who came for treatment to the Metabolic Endocrinology Polyclinic at Dr. M. Jamil Hospital. Analysis was performed on 132 subjects (66 T2DM patients and 66 controls). DNA was isolated from venous blood samples and amplified using the amplification refractory mutation system - polymerase chain reaction (ARMS-PCR) method to detect single nucleotide polymorphisms (SNPs) of TCF7L2 gene variant rs7895340. To determine the significance of association of SNPs of rs7895340 with T2DM in ethnic Minangkabau, data analysis was performed using chi-square test. RESULTS The ARMS - PCR for TCF7L2 gene variant rs7895340 found polymorphisms in 28 (42.42%) subjects with type 2 diabetes mellitus and in 15 (22.72%) non-diabetic subjects.There was a significant association between rs7895340 SNPs with the probability of T2DM in ethnic Minangkabau (OR=2.505, p=0.026). CONCLUSIONS Single nucleotide polymorphism rs7895340 of TCF7L2 gene can be used as genetic marker of type-2 diabetes mellitus in ethnic Minangkabau.http://www.univmed.org/wp-content/uploads/2015/02/Syamsurizal.pdfTCF7L2 geners7895340 SNPtype-2 diabetes mellitusethnic Minangkabau |
| spellingShingle | Syamsurizal Yanwirasti Asman Manaf Jamsari ML Edy Parwanto Arif Sardi Transcription factor 7-like 2 as type-2 diabetes mellitus diagnostic marker in ethnic Minangkabau Universa Medicina TCF7L2 gene rs7895340 SNP type-2 diabetes mellitus ethnic Minangkabau |
| title | Transcription factor 7-like 2 as type-2 diabetes mellitus diagnostic marker in ethnic Minangkabau |
| title_full | Transcription factor 7-like 2 as type-2 diabetes mellitus diagnostic marker in ethnic Minangkabau |
| title_fullStr | Transcription factor 7-like 2 as type-2 diabetes mellitus diagnostic marker in ethnic Minangkabau |
| title_full_unstemmed | Transcription factor 7-like 2 as type-2 diabetes mellitus diagnostic marker in ethnic Minangkabau |
| title_short | Transcription factor 7-like 2 as type-2 diabetes mellitus diagnostic marker in ethnic Minangkabau |
| title_sort | transcription factor 7 like 2 as type 2 diabetes mellitus diagnostic marker in ethnic minangkabau |
| topic | TCF7L2 gene rs7895340 SNP type-2 diabetes mellitus ethnic Minangkabau |
| url | http://www.univmed.org/wp-content/uploads/2015/02/Syamsurizal.pdf |
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