Protective effects of 17β-estradiol on high glucose-induced RPE cells
AIM: To discuss the protective effects and possible mechanisms of 17β-estradiol on human retinal pigment epithelial(RPE)cells induced by high glucose. <p>METHODS: RPE cells were cultured and divided into four groups according to randomized controlled method: blank control group: the cells were...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Press of International Journal of Ophthalmology (IJO PRESS)
2017-10-01
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| Series: | Guoji Yanke Zazhi |
| Subjects: | |
| Online Access: | http://ies.ijo.cn/cn_publish/2017/10/201710006.pdf |
| Summary: | AIM: To discuss the protective effects and possible mechanisms of 17β-estradiol on human retinal pigment epithelial(RPE)cells induced by high glucose. <p>METHODS: RPE cells were cultured and divided into four groups according to randomized controlled method: blank control group: the cells were treated with 5.5mmol/L routine glucose medium for processing; high glucose group: cells were treated with 100mmol/L glucose for 12h; 17β-estradiol low concentration group: after treated with 10 μmol/L 17β-estradiol, cells were treated with 100mmol/L glucose for 12h; 17β-estradiol high concentration group: after treated with 100 μmol/L 17β-estradiol, cells were treated with 100mmol/L glucose for 12h. Cell viability were tested by MTT colorimetric detection. Cells apoptosis were detected by Hochest33258 staining. Intracellular reactive oxygen species(ROS)level were detected by H<sub>2</sub>DCFDA staining. Expression of CAT, SOD and MDA were tested by colorimetric detection. <p>RESULTS: RPE cell activity decreased with the concentration of glucose increased; 17β-estradiol inhibited high glucose-induced cell viability decrease in RPE cells, decreased the apoptosis rate of RPE cells and intracellular ROS generation; besides, 17β-estradiol significantly increased the expression of CAT, SOD and decreased the expression of MDA in RPE cells. <p>CONCLUSION: The 17β-estradiol effectively inhibited high glucose -induced RPE cells damage, which provide reliable experimental basis for the treatment of injuries in RPE cells. |
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| ISSN: | 1672-5123 1672-5123 |