Protective effects of 17β-estradiol on high glucose-induced RPE cells
AIM: To discuss the protective effects and possible mechanisms of 17β-estradiol on human retinal pigment epithelial(RPE)cells induced by high glucose. <p>METHODS: RPE cells were cultured and divided into four groups according to randomized controlled method: blank control group: the cells were...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2017-10-01
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| Series: | Guoji Yanke Zazhi |
| Subjects: | |
| Online Access: | http://ies.ijo.cn/cn_publish/2017/10/201710006.pdf |
| _version_ | 1818586472086765568 |
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| author | Meng-Yao Jiao Yang-Yang Zhang Xuan Sun Kai Sun Xu-Cong Kang Wei Jiang Na Chen |
| author_facet | Meng-Yao Jiao Yang-Yang Zhang Xuan Sun Kai Sun Xu-Cong Kang Wei Jiang Na Chen |
| author_sort | Meng-Yao Jiao |
| collection | DOAJ |
| description | AIM: To discuss the protective effects and possible mechanisms of 17β-estradiol on human retinal pigment epithelial(RPE)cells induced by high glucose. <p>METHODS: RPE cells were cultured and divided into four groups according to randomized controlled method: blank control group: the cells were treated with 5.5mmol/L routine glucose medium for processing; high glucose group: cells were treated with 100mmol/L glucose for 12h; 17β-estradiol low concentration group: after treated with 10 μmol/L 17β-estradiol, cells were treated with 100mmol/L glucose for 12h; 17β-estradiol high concentration group: after treated with 100 μmol/L 17β-estradiol, cells were treated with 100mmol/L glucose for 12h. Cell viability were tested by MTT colorimetric detection. Cells apoptosis were detected by Hochest33258 staining. Intracellular reactive oxygen species(ROS)level were detected by H<sub>2</sub>DCFDA staining. Expression of CAT, SOD and MDA were tested by colorimetric detection. <p>RESULTS: RPE cell activity decreased with the concentration of glucose increased; 17β-estradiol inhibited high glucose-induced cell viability decrease in RPE cells, decreased the apoptosis rate of RPE cells and intracellular ROS generation; besides, 17β-estradiol significantly increased the expression of CAT, SOD and decreased the expression of MDA in RPE cells. <p>CONCLUSION: The 17β-estradiol effectively inhibited high glucose -induced RPE cells damage, which provide reliable experimental basis for the treatment of injuries in RPE cells. |
| first_indexed | 2024-12-16T08:53:30Z |
| format | Article |
| id | doaj.art-5bf0f13d2e2e4ff1885e041d26da735f |
| institution | Directory Open Access Journal |
| issn | 1672-5123 1672-5123 |
| language | English |
| last_indexed | 2024-12-16T08:53:30Z |
| publishDate | 2017-10-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | Guoji Yanke Zazhi |
| spelling | doaj.art-5bf0f13d2e2e4ff1885e041d26da735f2022-12-21T22:37:22ZengPress of International Journal of Ophthalmology (IJO PRESS)Guoji Yanke Zazhi1672-51231672-51232017-10-0117101830183310.3980/j.issn.1672-5123.2017.10.06Protective effects of 17β-estradiol on high glucose-induced RPE cellsMeng-Yao Jiao0Yang-Yang Zhang1Xuan Sun2Kai Sun3Xu-Cong Kang4Wei Jiang5Na Chen6Department of Ophthalmology, First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, ChinaDepartment of Ophthalmology, First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, ChinaDepartment of Ophthalmology, First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, ChinaDepartment of Ophthalmology, First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, ChinaDepartment of Ophthalmology, First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, ChinaDepartment of Ophthalmology, First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, ChinaDepartment of Ophthalmology, First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, ChinaAIM: To discuss the protective effects and possible mechanisms of 17β-estradiol on human retinal pigment epithelial(RPE)cells induced by high glucose. <p>METHODS: RPE cells were cultured and divided into four groups according to randomized controlled method: blank control group: the cells were treated with 5.5mmol/L routine glucose medium for processing; high glucose group: cells were treated with 100mmol/L glucose for 12h; 17β-estradiol low concentration group: after treated with 10 μmol/L 17β-estradiol, cells were treated with 100mmol/L glucose for 12h; 17β-estradiol high concentration group: after treated with 100 μmol/L 17β-estradiol, cells were treated with 100mmol/L glucose for 12h. Cell viability were tested by MTT colorimetric detection. Cells apoptosis were detected by Hochest33258 staining. Intracellular reactive oxygen species(ROS)level were detected by H<sub>2</sub>DCFDA staining. Expression of CAT, SOD and MDA were tested by colorimetric detection. <p>RESULTS: RPE cell activity decreased with the concentration of glucose increased; 17β-estradiol inhibited high glucose-induced cell viability decrease in RPE cells, decreased the apoptosis rate of RPE cells and intracellular ROS generation; besides, 17β-estradiol significantly increased the expression of CAT, SOD and decreased the expression of MDA in RPE cells. <p>CONCLUSION: The 17β-estradiol effectively inhibited high glucose -induced RPE cells damage, which provide reliable experimental basis for the treatment of injuries in RPE cells.http://ies.ijo.cn/cn_publish/2017/10/201710006.pdf17β-estradiolhuman lens epithelial cellshigh glucoseprotective effectprotective effect |
| spellingShingle | Meng-Yao Jiao Yang-Yang Zhang Xuan Sun Kai Sun Xu-Cong Kang Wei Jiang Na Chen Protective effects of 17β-estradiol on high glucose-induced RPE cells Guoji Yanke Zazhi 17β-estradiol human lens epithelial cells high glucose protective effect protective effect |
| title | Protective effects of 17β-estradiol on high glucose-induced RPE cells |
| title_full | Protective effects of 17β-estradiol on high glucose-induced RPE cells |
| title_fullStr | Protective effects of 17β-estradiol on high glucose-induced RPE cells |
| title_full_unstemmed | Protective effects of 17β-estradiol on high glucose-induced RPE cells |
| title_short | Protective effects of 17β-estradiol on high glucose-induced RPE cells |
| title_sort | protective effects of 17β estradiol on high glucose induced rpe cells |
| topic | 17β-estradiol human lens epithelial cells high glucose protective effect protective effect |
| url | http://ies.ijo.cn/cn_publish/2017/10/201710006.pdf |
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