Echocardiographic Findings in Canine Model of Chagas Disease Immunized with DNA <i>Trypanosoma cruzi</i> Genes
Chagas disease (ChD) is considered an emerging disease in the USA and Europe. <i>Trypanosoma cruzi</i> genes encoding a <i>trans</i>-sialidase protein and an amastigote-specific glycoprotein were tested as vaccines in canine model. The aim for this study was determining the p...
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MDPI AG
2020-04-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/4/648 |
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| author | Olivia Rodríguez-Morales Francisco-Javier Roldán Jesús Vargas-Barrón Enrique Parra-Benítez María de Lourdes Medina-García Emilia Vergara-Bello Minerva Arce-Fonseca |
| author_facet | Olivia Rodríguez-Morales Francisco-Javier Roldán Jesús Vargas-Barrón Enrique Parra-Benítez María de Lourdes Medina-García Emilia Vergara-Bello Minerva Arce-Fonseca |
| author_sort | Olivia Rodríguez-Morales |
| collection | DOAJ |
| description | Chagas disease (ChD) is considered an emerging disease in the USA and Europe. <i>Trypanosoma cruzi</i> genes encoding a <i>trans</i>-sialidase protein and an amastigote-specific glycoprotein were tested as vaccines in canine model. The aim for this study was determining the prophylactic effect of these genes in experimentally infected dogs by echocardiography evaluation to compare with our findings obtained by other techniques published previously. Low fractional-shortening values of non-vaccinated dogs suggested an impairment in general cardiac function. Low left ventricular ejection fraction values found in infected dogs suggested myocardial injury regardless of whether they were vaccinated. Low left ventricular diastolic/systolic diameters suggested that progressive heart damage or heart dilation could be prevented by DNA vaccination. Systolic peak time was higher in non-vaccinated groups, increasing vulnerability to malignant arrhythmias and sudden death. High left ventricular volume suggested a decrease in wall thickness that might lead to increased size of the heart cavity, except in the pBCSP plasmid-vaccinated dogs. There was an echocardiographic evidence of left ventricular dilation and reduction in systolic function in experimental chagasic dogs. Echocardiography allowed a more complete follow-up of the pathological process in the living patient than with other techniques like electrocardiography, anatomopathology, and histopathology, being the method of choice for characterizing the clinical stages of ChD. |
| first_indexed | 2024-03-10T20:35:12Z |
| format | Article |
| id | doaj.art-5bf166804cbf4e42a5d167437679f40e |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T20:35:12Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-5bf166804cbf4e42a5d167437679f40e2023-11-19T21:05:52ZengMDPI AGAnimals2076-26152020-04-0110464810.3390/ani10040648Echocardiographic Findings in Canine Model of Chagas Disease Immunized with DNA <i>Trypanosoma cruzi</i> GenesOlivia Rodríguez-Morales0Francisco-Javier Roldán1Jesús Vargas-Barrón2Enrique Parra-Benítez3María de Lourdes Medina-García4Emilia Vergara-Bello5Minerva Arce-Fonseca6Department of Molecular Biology, National Institute of Cardiology “Ignacio Chávez”, Juan Badiano No. 1, Col. Sección XVI, Tlalpan, Mexico City 14080, MexicoDepartment of Echocardiography, National Institute of Cardiology “Ignacio Chávez”, Juan Badiano No. 1, Col. Sección XVI, Tlalpan, Mexico City 14080, MexicoDepartment of Echocardiography, National Institute of Cardiology “Ignacio Chávez”, Juan Badiano No. 1, Col. Sección XVI, Tlalpan, Mexico City 14080, MexicoDepartment of Molecular Biology, National Institute of Cardiology “Ignacio Chávez”, Juan Badiano No. 1, Col. Sección XVI, Tlalpan, Mexico City 14080, MexicoDepartment of Molecular Biology, National Institute of Cardiology “Ignacio Chávez”, Juan Badiano No. 1, Col. Sección XVI, Tlalpan, Mexico City 14080, MexicoDepartment of Molecular Biology, National Institute of Cardiology “Ignacio Chávez”, Juan Badiano No. 1, Col. Sección XVI, Tlalpan, Mexico City 14080, MexicoDepartment of Molecular Biology, National Institute of Cardiology “Ignacio Chávez”, Juan Badiano No. 1, Col. Sección XVI, Tlalpan, Mexico City 14080, MexicoChagas disease (ChD) is considered an emerging disease in the USA and Europe. <i>Trypanosoma cruzi</i> genes encoding a <i>trans</i>-sialidase protein and an amastigote-specific glycoprotein were tested as vaccines in canine model. The aim for this study was determining the prophylactic effect of these genes in experimentally infected dogs by echocardiography evaluation to compare with our findings obtained by other techniques published previously. Low fractional-shortening values of non-vaccinated dogs suggested an impairment in general cardiac function. Low left ventricular ejection fraction values found in infected dogs suggested myocardial injury regardless of whether they were vaccinated. Low left ventricular diastolic/systolic diameters suggested that progressive heart damage or heart dilation could be prevented by DNA vaccination. Systolic peak time was higher in non-vaccinated groups, increasing vulnerability to malignant arrhythmias and sudden death. High left ventricular volume suggested a decrease in wall thickness that might lead to increased size of the heart cavity, except in the pBCSP plasmid-vaccinated dogs. There was an echocardiographic evidence of left ventricular dilation and reduction in systolic function in experimental chagasic dogs. Echocardiography allowed a more complete follow-up of the pathological process in the living patient than with other techniques like electrocardiography, anatomopathology, and histopathology, being the method of choice for characterizing the clinical stages of ChD.https://www.mdpi.com/2076-2615/10/4/648echocardiographyChagas disease<i>Trypanosoma cruzi</i>canine modelDNA vaccine |
| spellingShingle | Olivia Rodríguez-Morales Francisco-Javier Roldán Jesús Vargas-Barrón Enrique Parra-Benítez María de Lourdes Medina-García Emilia Vergara-Bello Minerva Arce-Fonseca Echocardiographic Findings in Canine Model of Chagas Disease Immunized with DNA <i>Trypanosoma cruzi</i> Genes Animals echocardiography Chagas disease <i>Trypanosoma cruzi</i> canine model DNA vaccine |
| title | Echocardiographic Findings in Canine Model of Chagas Disease Immunized with DNA <i>Trypanosoma cruzi</i> Genes |
| title_full | Echocardiographic Findings in Canine Model of Chagas Disease Immunized with DNA <i>Trypanosoma cruzi</i> Genes |
| title_fullStr | Echocardiographic Findings in Canine Model of Chagas Disease Immunized with DNA <i>Trypanosoma cruzi</i> Genes |
| title_full_unstemmed | Echocardiographic Findings in Canine Model of Chagas Disease Immunized with DNA <i>Trypanosoma cruzi</i> Genes |
| title_short | Echocardiographic Findings in Canine Model of Chagas Disease Immunized with DNA <i>Trypanosoma cruzi</i> Genes |
| title_sort | echocardiographic findings in canine model of chagas disease immunized with dna i trypanosoma cruzi i genes |
| topic | echocardiography Chagas disease <i>Trypanosoma cruzi</i> canine model DNA vaccine |
| url | https://www.mdpi.com/2076-2615/10/4/648 |
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