Proteomic and lipidomic analysis of exosomes derived from ovarian cancer cells and ovarian surface epithelial cells
Abstract Background The limitation of current biomarker of early stage ovarian cancer and the anatomical location of ovarian (depths of the pelvic) make ovarian cancer difficult to be detected in early stage. Growing evidence shows exosomes as key information transmitters, it carried molecules, such...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-01-01
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| Series: | Journal of Ovarian Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13048-020-0609-y |
| _version_ | 1797971899298349056 |
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| author | Lin Cheng Kun Zhang Yunan Qing Dong Li Manhua Cui Peng Jin Tianmin Xu |
| author_facet | Lin Cheng Kun Zhang Yunan Qing Dong Li Manhua Cui Peng Jin Tianmin Xu |
| author_sort | Lin Cheng |
| collection | DOAJ |
| description | Abstract Background The limitation of current biomarker of early stage ovarian cancer and the anatomical location of ovarian (depths of the pelvic) make ovarian cancer difficult to be detected in early stage. Growing evidence shows exosomes as key information transmitters, it carried molecules, such as miRNAs, proteins, lipids, double-stranded DNA have been reported as promising biomarkers in many diseases. However, little is known about the protein and lipid composition of ovarian cancer. Methods Here, we report proteomic and lipidomic analysis of exosomes derived from ovarian cancer cells (SKOV-3) and ovarian surface epithelial cells (HOSEPiC). Results A total of 1433 proteins and 1227 lipid species were identified from two cell line derived exosomes. Several lipid species and proteins significantly differ in SKOV-3 derived exosomes compared to those from HOSEPiC. For example, we noted that ChE and ZyE species were in general more abundant in exosomes from SKOV-3 than from HOSEPiC; Collagen type V alpha 2 chain (COL5A2) and lipoprotein lipase (LPL) were significantly higher in SKOV-3 derived exosomes than HOSEpic (p < 0.05). Conclusions Our research indicates the promising role of exosomal proteins and lipids in the early diagnosis of ovarian cancer. |
| first_indexed | 2024-04-11T03:38:48Z |
| format | Article |
| id | doaj.art-5bf1fdbaf16c49f39e5ae2b431452f96 |
| institution | Directory Open Access Journal |
| issn | 1757-2215 |
| language | English |
| last_indexed | 2024-04-11T03:38:48Z |
| publishDate | 2020-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj.art-5bf1fdbaf16c49f39e5ae2b431452f962023-01-02T04:15:52ZengBMCJournal of Ovarian Research1757-22152020-01-0113111310.1186/s13048-020-0609-yProteomic and lipidomic analysis of exosomes derived from ovarian cancer cells and ovarian surface epithelial cellsLin Cheng0Kun Zhang1Yunan Qing2Dong Li3Manhua Cui4Peng Jin5Tianmin Xu6The Second Hospital of Jilin UniversityThe Second Hospital of Jilin UniversityThe Second Hospital of Jilin UniversityThe Second Hospital of Jilin UniversityThe Second Hospital of Jilin UniversityDepartment of Human Genetics, Emory University School of MedicineThe Second Hospital of Jilin UniversityAbstract Background The limitation of current biomarker of early stage ovarian cancer and the anatomical location of ovarian (depths of the pelvic) make ovarian cancer difficult to be detected in early stage. Growing evidence shows exosomes as key information transmitters, it carried molecules, such as miRNAs, proteins, lipids, double-stranded DNA have been reported as promising biomarkers in many diseases. However, little is known about the protein and lipid composition of ovarian cancer. Methods Here, we report proteomic and lipidomic analysis of exosomes derived from ovarian cancer cells (SKOV-3) and ovarian surface epithelial cells (HOSEPiC). Results A total of 1433 proteins and 1227 lipid species were identified from two cell line derived exosomes. Several lipid species and proteins significantly differ in SKOV-3 derived exosomes compared to those from HOSEPiC. For example, we noted that ChE and ZyE species were in general more abundant in exosomes from SKOV-3 than from HOSEPiC; Collagen type V alpha 2 chain (COL5A2) and lipoprotein lipase (LPL) were significantly higher in SKOV-3 derived exosomes than HOSEpic (p < 0.05). Conclusions Our research indicates the promising role of exosomal proteins and lipids in the early diagnosis of ovarian cancer.https://doi.org/10.1186/s13048-020-0609-yOvarian cancerExosomeProteomicsLipidomics |
| spellingShingle | Lin Cheng Kun Zhang Yunan Qing Dong Li Manhua Cui Peng Jin Tianmin Xu Proteomic and lipidomic analysis of exosomes derived from ovarian cancer cells and ovarian surface epithelial cells Journal of Ovarian Research Ovarian cancer Exosome Proteomics Lipidomics |
| title | Proteomic and lipidomic analysis of exosomes derived from ovarian cancer cells and ovarian surface epithelial cells |
| title_full | Proteomic and lipidomic analysis of exosomes derived from ovarian cancer cells and ovarian surface epithelial cells |
| title_fullStr | Proteomic and lipidomic analysis of exosomes derived from ovarian cancer cells and ovarian surface epithelial cells |
| title_full_unstemmed | Proteomic and lipidomic analysis of exosomes derived from ovarian cancer cells and ovarian surface epithelial cells |
| title_short | Proteomic and lipidomic analysis of exosomes derived from ovarian cancer cells and ovarian surface epithelial cells |
| title_sort | proteomic and lipidomic analysis of exosomes derived from ovarian cancer cells and ovarian surface epithelial cells |
| topic | Ovarian cancer Exosome Proteomics Lipidomics |
| url | https://doi.org/10.1186/s13048-020-0609-y |
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