Peptide Self-Assembly Facilitating DNA Transfection and the Application in Inhibiting Cancer Cells
Non-viral vectors have been developing in gene delivery due to their safety and low immunogenicity. But their transfection effect is usually very low, thus limiting the application. Hence, we designed eight peptides (compounds <b>1</b>–<b>8</b>). We compared their performance...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/5/932 |
| _version_ | 1797264150681878528 |
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| author | Jingyu Wang Min Ye Baokuan Zhu |
| author_facet | Jingyu Wang Min Ye Baokuan Zhu |
| author_sort | Jingyu Wang |
| collection | DOAJ |
| description | Non-viral vectors have been developing in gene delivery due to their safety and low immunogenicity. But their transfection effect is usually very low, thus limiting the application. Hence, we designed eight peptides (compounds <b>1</b>–<b>8</b>). We compared their performances; compound <b>8</b> had the best transfection efficacy and biocompatibility. The transfection effect was similar with that of PEI, a most-widely-employed commercial transfection reagent. Atomic force microscope (AFM) images showed that the compound could self-assemble and the self-assembled peptide might encapsulate DNA. Based on these results, we further analyzed the inhibitory result in cancer cells and found that compound <b>8</b> could partially fight against Hela cells. Therefore, the compound is promising to pave the way for the development of more effective and less toxic transfection vectors. |
| first_indexed | 2024-04-25T00:24:20Z |
| format | Article |
| id | doaj.art-5bf2f34cf97041dfa8924296bea6a160 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-25T00:24:20Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-5bf2f34cf97041dfa8924296bea6a1602024-03-12T16:50:26ZengMDPI AGMolecules1420-30492024-02-0129593210.3390/molecules29050932Peptide Self-Assembly Facilitating DNA Transfection and the Application in Inhibiting Cancer CellsJingyu Wang0Min Ye1Baokuan Zhu2School of Biomedical Engineering and Technology, Tianjin Medical University, Tianjin 300070, ChinaCollege of Pharmacy, Southern Medical University, Guangzhou 510280, ChinaSchool of Biomedical Engineering and Technology, Tianjin Medical University, Tianjin 300070, ChinaNon-viral vectors have been developing in gene delivery due to their safety and low immunogenicity. But their transfection effect is usually very low, thus limiting the application. Hence, we designed eight peptides (compounds <b>1</b>–<b>8</b>). We compared their performances; compound <b>8</b> had the best transfection efficacy and biocompatibility. The transfection effect was similar with that of PEI, a most-widely-employed commercial transfection reagent. Atomic force microscope (AFM) images showed that the compound could self-assemble and the self-assembled peptide might encapsulate DNA. Based on these results, we further analyzed the inhibitory result in cancer cells and found that compound <b>8</b> could partially fight against Hela cells. Therefore, the compound is promising to pave the way for the development of more effective and less toxic transfection vectors.https://www.mdpi.com/1420-3049/29/5/932transfectionself-assembled peptidecancer cells |
| spellingShingle | Jingyu Wang Min Ye Baokuan Zhu Peptide Self-Assembly Facilitating DNA Transfection and the Application in Inhibiting Cancer Cells Molecules transfection self-assembled peptide cancer cells |
| title | Peptide Self-Assembly Facilitating DNA Transfection and the Application in Inhibiting Cancer Cells |
| title_full | Peptide Self-Assembly Facilitating DNA Transfection and the Application in Inhibiting Cancer Cells |
| title_fullStr | Peptide Self-Assembly Facilitating DNA Transfection and the Application in Inhibiting Cancer Cells |
| title_full_unstemmed | Peptide Self-Assembly Facilitating DNA Transfection and the Application in Inhibiting Cancer Cells |
| title_short | Peptide Self-Assembly Facilitating DNA Transfection and the Application in Inhibiting Cancer Cells |
| title_sort | peptide self assembly facilitating dna transfection and the application in inhibiting cancer cells |
| topic | transfection self-assembled peptide cancer cells |
| url | https://www.mdpi.com/1420-3049/29/5/932 |
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