Evaluation of oxidative stress in seminal plasma of adolescents with varicocele
Varicocele, defined by a dilation of efferent testicular veins, is the most commonly identifiable, surgically correctable lesion associated with male-factor infertility, starts at puberty and causes a progressive decline in fertility potential. The pathophysiology of infertility caused by this disea...
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| Format: | Article |
| Language: | English |
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Bioscientifica
2021-05-01
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| Series: | Reproduction and Fertility |
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| Online Access: | https://raf.bioscientifica.com/view/journals/raf/2/2/RAF-20-0048.xml |
| _version_ | 1818608849533272064 |
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| author | Valéria Barradas Mariana Pereira Antoniassi Paula Intasqui Marcilio Nichi Ricardo Pimenta Bertolla Deborah Montagnini Spaine |
| author_facet | Valéria Barradas Mariana Pereira Antoniassi Paula Intasqui Marcilio Nichi Ricardo Pimenta Bertolla Deborah Montagnini Spaine |
| author_sort | Valéria Barradas |
| collection | DOAJ |
| description | Varicocele, defined by a dilation of efferent testicular veins, is the most commonly identifiable, surgically correctable lesion associated with male-factor infertility, starts at puberty and causes a progressive decline in fertility potential. The pathophysiology of infertility caused by this disease is still poorly understood, but it is suggested that the main mechanism is oxidative stress. Therefore, the aim of this study was to verify if the varicocele is associated with changes in enzymatic antioxidant mechanisms and seminal plasma lipid peroxidation levels in adolescents. We recruited 90 adolescents that were divided into control (C; n = 27); varicocele and normal semen (VNS; n =46); varicocele and altered semen (VAS; n =17). Seminal and serum levels of lipid peroxidation were quantified by thiobarbituric acid reactive substances (TBARS). Seminal plasma antioxidant profile was evaluated by the activities of catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD). The VAS group had increased lipid peroxidation levels when compared to the other groups. The levels of serum lipid peroxidation and activities of the enzymes SOD and GPx did not differ between groups. CAT was undetectable by the method used. In conclusion, in adolescents with varicocele and altered semen analysis, there is an increase in seminal lipid peroxidation levels compared to adolescents with varicocele and without seminal change and adolescents without evident varicocele. However, the observed oxidative stress is not caused by a decrease in superoxide dismutase and glutathione peroxidase activities, which did not differ between adolescents with and without evident varicocele. |
| first_indexed | 2024-12-16T14:49:11Z |
| format | Article |
| id | doaj.art-5bf6c9c9bda34dc0970c7f2dec3c0bb6 |
| institution | Directory Open Access Journal |
| issn | 2633-8386 2633-8386 |
| language | English |
| last_indexed | 2024-12-16T14:49:11Z |
| publishDate | 2021-05-01 |
| publisher | Bioscientifica |
| record_format | Article |
| series | Reproduction and Fertility |
| spelling | doaj.art-5bf6c9c9bda34dc0970c7f2dec3c0bb62022-12-21T22:27:40ZengBioscientificaReproduction and Fertility2633-83862633-83862021-05-0122141150https://doi.org/10.1530/RAF-20-0048Evaluation of oxidative stress in seminal plasma of adolescents with varicoceleValéria Barradas0Mariana Pereira Antoniassi1Paula Intasqui2Marcilio Nichi3Ricardo Pimenta Bertolla4Deborah Montagnini Spaine5Department of Surgery, Division of Urology, Human Reproduction Section, Universidade Federal de São Paulo, São Paulo, BrazilDepartment of Surgery, Division of Urology, Human Reproduction Section, Universidade Federal de São Paulo, São Paulo, BrazilDepartment of Surgery, Division of Urology, Human Reproduction Section, Universidade Federal de São Paulo, São Paulo, BrazilDepartment of Animal Reproduction, School of Veterinary Medicine and Animal Sciences, University of Sao Paulo, São Paulo, BrazilDepartment of Surgery, Division of Urology, Human Reproduction Section, Universidade Federal de São Paulo, São Paulo, Brazil; Hospital São Paulo, São Paulo, Brazil Department of Surgery, Division of Urology, Human Reproduction Section, Universidade Federal de São Paulo, São Paulo, BrazilVaricocele, defined by a dilation of efferent testicular veins, is the most commonly identifiable, surgically correctable lesion associated with male-factor infertility, starts at puberty and causes a progressive decline in fertility potential. The pathophysiology of infertility caused by this disease is still poorly understood, but it is suggested that the main mechanism is oxidative stress. Therefore, the aim of this study was to verify if the varicocele is associated with changes in enzymatic antioxidant mechanisms and seminal plasma lipid peroxidation levels in adolescents. We recruited 90 adolescents that were divided into control (C; n = 27); varicocele and normal semen (VNS; n =46); varicocele and altered semen (VAS; n =17). Seminal and serum levels of lipid peroxidation were quantified by thiobarbituric acid reactive substances (TBARS). Seminal plasma antioxidant profile was evaluated by the activities of catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD). The VAS group had increased lipid peroxidation levels when compared to the other groups. The levels of serum lipid peroxidation and activities of the enzymes SOD and GPx did not differ between groups. CAT was undetectable by the method used. In conclusion, in adolescents with varicocele and altered semen analysis, there is an increase in seminal lipid peroxidation levels compared to adolescents with varicocele and without seminal change and adolescents without evident varicocele. However, the observed oxidative stress is not caused by a decrease in superoxide dismutase and glutathione peroxidase activities, which did not differ between adolescents with and without evident varicocele.https://raf.bioscientifica.com/view/journals/raf/2/2/RAF-20-0048.xmlvaricoceleoxidative stressantioxidantsadolescentseminal analysis |
| spellingShingle | Valéria Barradas Mariana Pereira Antoniassi Paula Intasqui Marcilio Nichi Ricardo Pimenta Bertolla Deborah Montagnini Spaine Evaluation of oxidative stress in seminal plasma of adolescents with varicocele Reproduction and Fertility varicocele oxidative stress antioxidants adolescent seminal analysis |
| title | Evaluation of oxidative stress in seminal plasma of adolescents with varicocele |
| title_full | Evaluation of oxidative stress in seminal plasma of adolescents with varicocele |
| title_fullStr | Evaluation of oxidative stress in seminal plasma of adolescents with varicocele |
| title_full_unstemmed | Evaluation of oxidative stress in seminal plasma of adolescents with varicocele |
| title_short | Evaluation of oxidative stress in seminal plasma of adolescents with varicocele |
| title_sort | evaluation of oxidative stress in seminal plasma of adolescents with varicocele |
| topic | varicocele oxidative stress antioxidants adolescent seminal analysis |
| url | https://raf.bioscientifica.com/view/journals/raf/2/2/RAF-20-0048.xml |
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