S100A9 promotes apical-basal polarity and neural tube formation during differentiation of embryonic stem cells into neural progenitor cells
Objective To explore the role of S100A9 in the apical-basal polarity (ABP) and neural tube formation during the differentiation of embryonic stem cells (ESCs) into neural progenitor cells (NPCs) in vitro. Methods Immunofluorescence assay was used to observe the luminal arrangement, expression of Nes...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Journal of Army Medical University
2023-01-01
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| Series: | 陆军军医大学学报 |
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| Online Access: | http://202.202.232.58/Upload/rhtml/202204002.htm |
| _version_ | 1797955487813074944 |
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| author | ZHANG Xiang YUE Jianhe HUANG Ning |
| author_facet | ZHANG Xiang YUE Jianhe HUANG Ning |
| author_sort | ZHANG Xiang |
| collection | DOAJ |
| description | Objective To explore the role of S100A9 in the apical-basal polarity (ABP) and neural tube formation during the differentiation of embryonic stem cells (ESCs) into neural progenitor cells (NPCs) in vitro. Methods Immunofluorescence assay was used to observe the luminal arrangement, expression of Nestin and paired box protein 6 (Pax-6), and localization of S100A9, N-cadherin and Zonula occludens 1 (ZO-1) on days 1, 2, 3 and 6 of the ESCs differentiation into NPCs. In addition, the ESCs were treated with S100A9-downregulation lentivirus as well as subsequential exogenous S100A9 recombinant protein, then the localization changes of N-cadherin and ZO-1 were detected. The S100A9 expression in different time points during ESCs differentiation process was measured by Western blotting, and the effects of S100A9 on Notch1 and downstream centromere binding factor 1 (CBF1) were also tested. Results Nestin and Pax-6 were positively expressed after ESCs differentiation for 3 d, N-cadherin and ZO-1 were gathered at the apical side and presented the characteristic neural rosette structure of ABP, and the neural tubes were formed on day 6. The expression of S100A9 was upregulated significantly from day 3 after differentiation (P < 0.01) and was located on the apical side. Although ESCs were still able to differentiate into NPCs after down-regulation of S100A9, the number of neural rosettes was greatly reduced (P < 0.01), which could be partially restored by supplement of exogenous S100A9 recombinant protein (P < 0.01). Furthermore, declined S100A9 inhibited the expression of Notch 1 and downstream CBF1 during differentiation. Conclusion S100A9 may contribute to the ABP and the formation of neural lumen during the differentiation of ESCs into NPCs by regulating Notch 1/CBF1.
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| first_indexed | 2024-04-10T23:34:54Z |
| format | Article |
| id | doaj.art-5bf8480ac63e483fb033eb6294f1fbc6 |
| institution | Directory Open Access Journal |
| issn | 2097-0927 |
| language | zho |
| last_indexed | 2024-04-10T23:34:54Z |
| publishDate | 2023-01-01 |
| publisher | Editorial Office of Journal of Army Medical University |
| record_format | Article |
| series | 陆军军医大学学报 |
| spelling | doaj.art-5bf8480ac63e483fb033eb6294f1fbc62023-01-12T00:08:40ZzhoEditorial Office of Journal of Army Medical University陆军军医大学学报2097-09272023-01-01451101810.16016/j.2097-0927.202204002S100A9 promotes apical-basal polarity and neural tube formation during differentiation of embryonic stem cells into neural progenitor cellsZHANG Xiang0YUE Jianhe1HUANG Ning2Department of Neurosurgery, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, ChinaDepartment of Neurosurgery, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, ChinaDepartment of Neurosurgery, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, ChinaObjective To explore the role of S100A9 in the apical-basal polarity (ABP) and neural tube formation during the differentiation of embryonic stem cells (ESCs) into neural progenitor cells (NPCs) in vitro. Methods Immunofluorescence assay was used to observe the luminal arrangement, expression of Nestin and paired box protein 6 (Pax-6), and localization of S100A9, N-cadherin and Zonula occludens 1 (ZO-1) on days 1, 2, 3 and 6 of the ESCs differentiation into NPCs. In addition, the ESCs were treated with S100A9-downregulation lentivirus as well as subsequential exogenous S100A9 recombinant protein, then the localization changes of N-cadherin and ZO-1 were detected. The S100A9 expression in different time points during ESCs differentiation process was measured by Western blotting, and the effects of S100A9 on Notch1 and downstream centromere binding factor 1 (CBF1) were also tested. Results Nestin and Pax-6 were positively expressed after ESCs differentiation for 3 d, N-cadherin and ZO-1 were gathered at the apical side and presented the characteristic neural rosette structure of ABP, and the neural tubes were formed on day 6. The expression of S100A9 was upregulated significantly from day 3 after differentiation (P < 0.01) and was located on the apical side. Although ESCs were still able to differentiate into NPCs after down-regulation of S100A9, the number of neural rosettes was greatly reduced (P < 0.01), which could be partially restored by supplement of exogenous S100A9 recombinant protein (P < 0.01). Furthermore, declined S100A9 inhibited the expression of Notch 1 and downstream CBF1 during differentiation. Conclusion S100A9 may contribute to the ABP and the formation of neural lumen during the differentiation of ESCs into NPCs by regulating Notch 1/CBF1. http://202.202.232.58/Upload/rhtml/202204002.htmembryonic stem cellsneural progenitor cellsapical-basal polarityneural tubes100a9 |
| spellingShingle | ZHANG Xiang YUE Jianhe HUANG Ning S100A9 promotes apical-basal polarity and neural tube formation during differentiation of embryonic stem cells into neural progenitor cells 陆军军医大学学报 embryonic stem cells neural progenitor cells apical-basal polarity neural tube s100a9 |
| title | S100A9 promotes apical-basal polarity and neural tube formation during differentiation of embryonic stem cells into neural progenitor cells |
| title_full | S100A9 promotes apical-basal polarity and neural tube formation during differentiation of embryonic stem cells into neural progenitor cells |
| title_fullStr | S100A9 promotes apical-basal polarity and neural tube formation during differentiation of embryonic stem cells into neural progenitor cells |
| title_full_unstemmed | S100A9 promotes apical-basal polarity and neural tube formation during differentiation of embryonic stem cells into neural progenitor cells |
| title_short | S100A9 promotes apical-basal polarity and neural tube formation during differentiation of embryonic stem cells into neural progenitor cells |
| title_sort | s100a9 promotes apical basal polarity and neural tube formation during differentiation of embryonic stem cells into neural progenitor cells |
| topic | embryonic stem cells neural progenitor cells apical-basal polarity neural tube s100a9 |
| url | http://202.202.232.58/Upload/rhtml/202204002.htm |
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