<i>Mycobacterium chimaera</i> Identification Using MALDI-TOF MS Technology: A Practical Approach for the Clinical Microbiology Laboratories
<i>Mycobacterium chimaera</i> (MC) is an environmental, slowly growing, non-tuberculous mycobacterium (NTM) belonging to <i>Mycobacterium avium</i> complex (MAC), which recently has been linked to severe cardiovascular infections following open heart and vascular surgery. The...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/6/1184 |
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| author | Jessica Bagnarino Daniela Barbarini Giuseppe Russello Mariangela Siciliano Vincenzina Monzillo Fausto Baldanti Edoardo Carretto |
| author_facet | Jessica Bagnarino Daniela Barbarini Giuseppe Russello Mariangela Siciliano Vincenzina Monzillo Fausto Baldanti Edoardo Carretto |
| author_sort | Jessica Bagnarino |
| collection | DOAJ |
| description | <i>Mycobacterium chimaera</i> (MC) is an environmental, slowly growing, non-tuberculous mycobacterium (NTM) belonging to <i>Mycobacterium avium</i> complex (MAC), which recently has been linked to severe cardiovascular infections following open heart and vascular surgery. The majority of the diagnostic laboratory tests used in routine are not able to distinguish MC from <i>M. intracellulare</i> (MI), because of the great genetic similarity existing between these two species. The Genotype Mycobacterium NTM-DR™ represents a valid method to differentiate between these species, but it is expensive, requiring also specialized personnel. Recently, MALDI-TOF MS has been proposed to identify relevant NTM. However, a software implementation is required to distinguish between MC and MI, presenting the two microorganisms’ overlapping spectra. The present study evaluates the feasibility of applying a MALDI-TOF logarithmic-based analysis in the routine of a clinical microbiology laboratory, and proposes an easy-to-use template spreadsheet to make the results quickly interpretable. The protocol was previously validated through the identification of 87 strains of MC/MI collected from clinical and environmental samples, and it was identified using the GenoType Mycobacterium NTM-DR™ and/or WGS. The proposed protocol provides accurate identification for the isolates tested; moreover, it is less expensive and more rapid than sequencing methods and can be implemented with minimum effort. |
| first_indexed | 2024-03-09T22:59:59Z |
| format | Article |
| id | doaj.art-5bf9af0394f04bed94ef6f45a3e1ea58 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T22:59:59Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-5bf9af0394f04bed94ef6f45a3e1ea582023-11-23T18:04:23ZengMDPI AGMicroorganisms2076-26072022-06-01106118410.3390/microorganisms10061184<i>Mycobacterium chimaera</i> Identification Using MALDI-TOF MS Technology: A Practical Approach for the Clinical Microbiology LaboratoriesJessica Bagnarino0Daniela Barbarini1Giuseppe Russello2Mariangela Siciliano3Vincenzina Monzillo4Fausto Baldanti5Edoardo Carretto6Microbiology and Virology Unit, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyMicrobiology and Virology Unit, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyClinical Microbiology Laboratory, Arcispedale S. Maria Nuova, IRCCS Azienda Unità Sanitaria Locale, 42122 Reggio Emilia, ItalyMicrobiology and Virology Unit, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyMicrobiology and Virology Unit, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyMicrobiology and Virology Unit, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyClinical Microbiology Laboratory, Arcispedale S. Maria Nuova, IRCCS Azienda Unità Sanitaria Locale, 42122 Reggio Emilia, Italy<i>Mycobacterium chimaera</i> (MC) is an environmental, slowly growing, non-tuberculous mycobacterium (NTM) belonging to <i>Mycobacterium avium</i> complex (MAC), which recently has been linked to severe cardiovascular infections following open heart and vascular surgery. The majority of the diagnostic laboratory tests used in routine are not able to distinguish MC from <i>M. intracellulare</i> (MI), because of the great genetic similarity existing between these two species. The Genotype Mycobacterium NTM-DR™ represents a valid method to differentiate between these species, but it is expensive, requiring also specialized personnel. Recently, MALDI-TOF MS has been proposed to identify relevant NTM. However, a software implementation is required to distinguish between MC and MI, presenting the two microorganisms’ overlapping spectra. The present study evaluates the feasibility of applying a MALDI-TOF logarithmic-based analysis in the routine of a clinical microbiology laboratory, and proposes an easy-to-use template spreadsheet to make the results quickly interpretable. The protocol was previously validated through the identification of 87 strains of MC/MI collected from clinical and environmental samples, and it was identified using the GenoType Mycobacterium NTM-DR™ and/or WGS. The proposed protocol provides accurate identification for the isolates tested; moreover, it is less expensive and more rapid than sequencing methods and can be implemented with minimum effort.https://www.mdpi.com/2076-2607/10/6/1184<i>Mycobacterium chimaera</i><i>Mycobacterium avium</i> complexidentificationMALDI-TOFdiagnosis |
| spellingShingle | Jessica Bagnarino Daniela Barbarini Giuseppe Russello Mariangela Siciliano Vincenzina Monzillo Fausto Baldanti Edoardo Carretto <i>Mycobacterium chimaera</i> Identification Using MALDI-TOF MS Technology: A Practical Approach for the Clinical Microbiology Laboratories Microorganisms <i>Mycobacterium chimaera</i> <i>Mycobacterium avium</i> complex identification MALDI-TOF diagnosis |
| title | <i>Mycobacterium chimaera</i> Identification Using MALDI-TOF MS Technology: A Practical Approach for the Clinical Microbiology Laboratories |
| title_full | <i>Mycobacterium chimaera</i> Identification Using MALDI-TOF MS Technology: A Practical Approach for the Clinical Microbiology Laboratories |
| title_fullStr | <i>Mycobacterium chimaera</i> Identification Using MALDI-TOF MS Technology: A Practical Approach for the Clinical Microbiology Laboratories |
| title_full_unstemmed | <i>Mycobacterium chimaera</i> Identification Using MALDI-TOF MS Technology: A Practical Approach for the Clinical Microbiology Laboratories |
| title_short | <i>Mycobacterium chimaera</i> Identification Using MALDI-TOF MS Technology: A Practical Approach for the Clinical Microbiology Laboratories |
| title_sort | i mycobacterium chimaera i identification using maldi tof ms technology a practical approach for the clinical microbiology laboratories |
| topic | <i>Mycobacterium chimaera</i> <i>Mycobacterium avium</i> complex identification MALDI-TOF diagnosis |
| url | https://www.mdpi.com/2076-2607/10/6/1184 |
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