Production and characterization of poly-3-hydroxybutyrate from crude glycerol by Bacillus sphaericus NII 0838 and improving its thermal properties by blending with other polymers
The aim of this work was to study the production of poly-3-hydroxybutyrate (PHB) under nitrogen limited conditions by Bacillus sphaericus NII 0838 using crude glycerol from biodiesel industry as sole carbon source. Effect of various process parameters on PHB production such as glycerol concentration...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2011-08-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400019 |
| _version_ | 1819264116000292864 |
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| author | Raveendran Sindhu Balakrishnan Ammu Parameswaran Binod Sreelatha K. Deepthi K. B. Ramachandran Carlos Ricardo Soccol Ashok Pandey |
| author_facet | Raveendran Sindhu Balakrishnan Ammu Parameswaran Binod Sreelatha K. Deepthi K. B. Ramachandran Carlos Ricardo Soccol Ashok Pandey |
| author_sort | Raveendran Sindhu |
| collection | DOAJ |
| description | The aim of this work was to study the production of poly-3-hydroxybutyrate (PHB) under nitrogen limited conditions by Bacillus sphaericus NII 0838 using crude glycerol from biodiesel industry as sole carbon source. Effect of various process parameters on PHB production such as glycerol concentration, inoculum size and pH of the medium were optimized. Characterization of extracted PHB was carried out by FT-IR, ¹H and 13C NMR. Results showed that the bacterial culture accumulated about 31% PHB in crude glycerol medium. The extracted PHB was blended with other polymers to improve its physical characteristics. The thermal properties of the polymer like melting temperature (Tm) and heat of fusion (ΔHf) were determined using DSC. |
| first_indexed | 2024-12-23T20:24:22Z |
| format | Article |
| id | doaj.art-5bfa3e2ed0f94bffa4e412bd031d5742 |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-12-23T20:24:22Z |
| publishDate | 2011-08-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-5bfa3e2ed0f94bffa4e412bd031d57422022-12-21T17:32:26ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242011-08-0154478379410.1590/S1516-89132011000400019Production and characterization of poly-3-hydroxybutyrate from crude glycerol by Bacillus sphaericus NII 0838 and improving its thermal properties by blending with other polymersRaveendran SindhuBalakrishnan AmmuParameswaran BinodSreelatha K. DeepthiK. B. RamachandranCarlos Ricardo SoccolAshok PandeyThe aim of this work was to study the production of poly-3-hydroxybutyrate (PHB) under nitrogen limited conditions by Bacillus sphaericus NII 0838 using crude glycerol from biodiesel industry as sole carbon source. Effect of various process parameters on PHB production such as glycerol concentration, inoculum size and pH of the medium were optimized. Characterization of extracted PHB was carried out by FT-IR, ¹H and 13C NMR. Results showed that the bacterial culture accumulated about 31% PHB in crude glycerol medium. The extracted PHB was blended with other polymers to improve its physical characteristics. The thermal properties of the polymer like melting temperature (Tm) and heat of fusion (ΔHf) were determined using DSC.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400019polyhydroxybutyrateBacillus sphaericuspolymer blendscrude glycerolbiodiesel industry |
| spellingShingle | Raveendran Sindhu Balakrishnan Ammu Parameswaran Binod Sreelatha K. Deepthi K. B. Ramachandran Carlos Ricardo Soccol Ashok Pandey Production and characterization of poly-3-hydroxybutyrate from crude glycerol by Bacillus sphaericus NII 0838 and improving its thermal properties by blending with other polymers Brazilian Archives of Biology and Technology polyhydroxybutyrate Bacillus sphaericus polymer blends crude glycerol biodiesel industry |
| title | Production and characterization of poly-3-hydroxybutyrate from crude glycerol by Bacillus sphaericus NII 0838 and improving its thermal properties by blending with other polymers |
| title_full | Production and characterization of poly-3-hydroxybutyrate from crude glycerol by Bacillus sphaericus NII 0838 and improving its thermal properties by blending with other polymers |
| title_fullStr | Production and characterization of poly-3-hydroxybutyrate from crude glycerol by Bacillus sphaericus NII 0838 and improving its thermal properties by blending with other polymers |
| title_full_unstemmed | Production and characterization of poly-3-hydroxybutyrate from crude glycerol by Bacillus sphaericus NII 0838 and improving its thermal properties by blending with other polymers |
| title_short | Production and characterization of poly-3-hydroxybutyrate from crude glycerol by Bacillus sphaericus NII 0838 and improving its thermal properties by blending with other polymers |
| title_sort | production and characterization of poly 3 hydroxybutyrate from crude glycerol by bacillus sphaericus nii 0838 and improving its thermal properties by blending with other polymers |
| topic | polyhydroxybutyrate Bacillus sphaericus polymer blends crude glycerol biodiesel industry |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400019 |
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