Genomic Characterization of β-Glucuronidase–Positive Escherichia coli O157:H7 Producing Stx2a
Among Shiga toxin (Stx)–producing Escherichia coli (STEC) O157:H7 strains, those producing Stx2a cause more severe diseases. Atypical STEC O157:H7 strains showing a β-glucuronidase–positive phenotype (GP STEC O157:H7) have rarely been isolated from humans, mostly from persons with asymptomatic or mi...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2018-12-01
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| Series: | Emerging Infectious Diseases |
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| Online Access: | https://wwwnc.cdc.gov/eid/article/24/12/18-0404_article |
| _version_ | 1818326853060919296 |
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| author | Yoshitoshi Ogura Kazuko Seto Yo Morimoto Keiji Nakamura Mitsuhiko P. Sato Yasuhiro Gotoh Takehiko Itoh Atsushi Toyoda Makoto Ohnishi Tetsuya Hayashi |
| author_facet | Yoshitoshi Ogura Kazuko Seto Yo Morimoto Keiji Nakamura Mitsuhiko P. Sato Yasuhiro Gotoh Takehiko Itoh Atsushi Toyoda Makoto Ohnishi Tetsuya Hayashi |
| author_sort | Yoshitoshi Ogura |
| collection | DOAJ |
| description | Among Shiga toxin (Stx)–producing Escherichia coli (STEC) O157:H7 strains, those producing Stx2a cause more severe diseases. Atypical STEC O157:H7 strains showing a β-glucuronidase–positive phenotype (GP STEC O157:H7) have rarely been isolated from humans, mostly from persons with asymptomatic or mild infections; Stx2a-producing strains have not been reported. We isolated, from a patient with bloody diarrhea, a GP STEC O157:H7 strain (PV15-279) that produces Stx2a in addition to Stx1a and Stx2c. Genomic comparison with other STEC O157 strains revealed that PV15-279 recently emerged from the stx1a/stx2c-positive GP STEC O157:H7 clone circulating in Japan. Major virulence genes are shared between typical (β-glucuronidase–negative) and GP STEC O157:H7 strains, and the Stx2-producing ability of PV15-279 is comparable to that of typical STEC O157:H7 strains; therefore, PV15-279 presents a virulence potential similar to that of typical STEC O157:H7. This study reveals the importance of GP O157:H7 as a source of highly pathogenic STEC clones. |
| first_indexed | 2024-12-13T12:06:58Z |
| format | Article |
| id | doaj.art-5bfc9acb604c4160a41e76dc1835fd15 |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-13T12:06:58Z |
| publishDate | 2018-12-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-5bfc9acb604c4160a41e76dc1835fd152022-12-21T23:46:55ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592018-12-0124122219222710.3201/eid2412.180404Genomic Characterization of β-Glucuronidase–Positive Escherichia coli O157:H7 Producing Stx2aYoshitoshi OguraKazuko SetoYo MorimotoKeiji NakamuraMitsuhiko P. SatoYasuhiro GotohTakehiko ItohAtsushi ToyodaMakoto OhnishiTetsuya HayashiAmong Shiga toxin (Stx)–producing Escherichia coli (STEC) O157:H7 strains, those producing Stx2a cause more severe diseases. Atypical STEC O157:H7 strains showing a β-glucuronidase–positive phenotype (GP STEC O157:H7) have rarely been isolated from humans, mostly from persons with asymptomatic or mild infections; Stx2a-producing strains have not been reported. We isolated, from a patient with bloody diarrhea, a GP STEC O157:H7 strain (PV15-279) that produces Stx2a in addition to Stx1a and Stx2c. Genomic comparison with other STEC O157 strains revealed that PV15-279 recently emerged from the stx1a/stx2c-positive GP STEC O157:H7 clone circulating in Japan. Major virulence genes are shared between typical (β-glucuronidase–negative) and GP STEC O157:H7 strains, and the Stx2-producing ability of PV15-279 is comparable to that of typical STEC O157:H7 strains; therefore, PV15-279 presents a virulence potential similar to that of typical STEC O157:H7. This study reveals the importance of GP O157:H7 as a source of highly pathogenic STEC clones.https://wwwnc.cdc.gov/eid/article/24/12/18-0404_articleEscherichia coliGP STEC O157:H7genomeShiga toxinStx2abacteria |
| spellingShingle | Yoshitoshi Ogura Kazuko Seto Yo Morimoto Keiji Nakamura Mitsuhiko P. Sato Yasuhiro Gotoh Takehiko Itoh Atsushi Toyoda Makoto Ohnishi Tetsuya Hayashi Genomic Characterization of β-Glucuronidase–Positive Escherichia coli O157:H7 Producing Stx2a Emerging Infectious Diseases Escherichia coli GP STEC O157:H7 genome Shiga toxin Stx2a bacteria |
| title | Genomic Characterization of β-Glucuronidase–Positive Escherichia coli O157:H7 Producing Stx2a |
| title_full | Genomic Characterization of β-Glucuronidase–Positive Escherichia coli O157:H7 Producing Stx2a |
| title_fullStr | Genomic Characterization of β-Glucuronidase–Positive Escherichia coli O157:H7 Producing Stx2a |
| title_full_unstemmed | Genomic Characterization of β-Glucuronidase–Positive Escherichia coli O157:H7 Producing Stx2a |
| title_short | Genomic Characterization of β-Glucuronidase–Positive Escherichia coli O157:H7 Producing Stx2a |
| title_sort | genomic characterization of β glucuronidase positive escherichia coli o157 h7 producing stx2a |
| topic | Escherichia coli GP STEC O157:H7 genome Shiga toxin Stx2a bacteria |
| url | https://wwwnc.cdc.gov/eid/article/24/12/18-0404_article |
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