Role of LncRNA H19 in promoting endometrial deciduation
Objective To explore the effect of LncRNA H19 on endometrial decidualization. Methods The expression of LncRNA H19 in decidual tissues of spontaneous abortion and induced abortion was detected by RT-qPCR; The expression of LncRNA H19 in non-pregnant endometrium and decidual tissues of different gest...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Journal of Army Medical University
2023-08-01
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| Series: | 陆军军医大学学报 |
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| Online Access: | http://aammt.tmmu.edu.cn/html/202303092.htm |
| _version_ | 1797731221213544448 |
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| author | WANG Tingting HUANG Jinglin MAO Yanhua ZHANG Yingfeng SUN Congcong |
| author_facet | WANG Tingting HUANG Jinglin MAO Yanhua ZHANG Yingfeng SUN Congcong |
| author_sort | WANG Tingting |
| collection | DOAJ |
| description | Objective To explore the effect of LncRNA H19 on endometrial decidualization. Methods The expression of LncRNA H19 in decidual tissues of spontaneous abortion and induced abortion was detected by RT-qPCR; The expression of LncRNA H19 in non-pregnant endometrium and decidual tissues of different gestational weeks was detected; Using MPA and 8-Br-cAMP inducers to induce the transformed human endometrial stromal cells (THESCs), RT-qPCR was used to detect the expression of prolactin (PRL) and insulin-like growth factor binding protein 1 (IGFBP-1); The expression changes of LncRNA H19 and FOXO1 were detected after the process of decidualization; Lentivirus was used to overexpress and interfere with LncRNA H19 in THESCs, and the proliferation of THESCs was detected by CCK-8 assay. After successful lentivirus transfection, decidualization was induced. RT-qPCR was used to detect the expression of PRL, IGFBP-1 and FOXO1 in blank control group, LV-GFP induction group, LV-H19-RNAi induction group and LV-H19 induction group. Results The expression of LncRNA H19 in spontaneous abortion group was lower than that in induced abortion group (P < 0.01); LncRNA H19 reached its peak at the 7th week of decidual tissue in early pregnancy (P < 0.01); After the decidualization of THESCs, the levels of PRL, IGFBP-1, FOXO1 and LncRNA H19 were higher than those of the control group (P < 0.01).The results of CCK-8 assay showed that the proliferation of THESCs was inhibited after the interference with LncRNA H19 (P < 0.01), while the proliferation of THESCs was promoted after the overexpression of LncRNA H19 (P < 0.01). The decidualization induction was performed, after interfering with and overexpressing the LncRNA H19 of THESCs. Compared with the blank control group, the LV-GFP induction group showed the increased mRNA expression of PRL (P < 0.05), IGFBP-1 (P < 0.01) and FOXO1 (P < 0.01). Compared with LV-GFP induction group, the mRNA expression of PRL (P < 0.05) and IGFBP-1 (P < 0.01) in LV-H19 induction group was increased, while the mRNA expression of PRL (P < 0.05), IGFBP-1 (P < 0.01) and FOXO1 (P < 0.01) in LV-H19-RNAi induction group was decreased. Conclusion LncRNA H19 promotes the proliferation and decidualization of endometrial stromal cells.
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| first_indexed | 2024-03-12T11:55:40Z |
| format | Article |
| id | doaj.art-5bfcaab602a746e99ef738b22525addb |
| institution | Directory Open Access Journal |
| issn | 2097-0927 |
| language | zho |
| last_indexed | 2024-03-12T11:55:40Z |
| publishDate | 2023-08-01 |
| publisher | Editorial Office of Journal of Army Medical University |
| record_format | Article |
| series | 陆军军医大学学报 |
| spelling | doaj.art-5bfcaab602a746e99ef738b22525addb2023-08-31T01:13:17ZzhoEditorial Office of Journal of Army Medical University陆军军医大学学报2097-09272023-08-0145161722173010.16016/j.2097-0927.202303092Role of LncRNA H19 in promoting endometrial deciduationWANG Tingting0HUANG Jinglin1MAO Yanhua2ZHANG Yingfeng3SUN Congcong4Department of Obstetrics and Gynecology, the University-Town Hospital Affiliated to Chongqing Medical University, Chongqing, 401331, ChinaDepartment of Obstetrics and Gynecology, the University-Town Hospital Affiliated to Chongqing Medical University, Chongqing, 401331, ChinaDepartment of Obstetrics and Gynecology, the University-Town Hospital Affiliated to Chongqing Medical University, Chongqing, 401331, ChinaDepartment of Obstetrics and Gynecology, the University-Town Hospital Affiliated to Chongqing Medical University, Chongqing, 401331, ChinaDepartment of Obstetrics and Gynecology, the University-Town Hospital Affiliated to Chongqing Medical University, Chongqing, 401331, ChinaObjective To explore the effect of LncRNA H19 on endometrial decidualization. Methods The expression of LncRNA H19 in decidual tissues of spontaneous abortion and induced abortion was detected by RT-qPCR; The expression of LncRNA H19 in non-pregnant endometrium and decidual tissues of different gestational weeks was detected; Using MPA and 8-Br-cAMP inducers to induce the transformed human endometrial stromal cells (THESCs), RT-qPCR was used to detect the expression of prolactin (PRL) and insulin-like growth factor binding protein 1 (IGFBP-1); The expression changes of LncRNA H19 and FOXO1 were detected after the process of decidualization; Lentivirus was used to overexpress and interfere with LncRNA H19 in THESCs, and the proliferation of THESCs was detected by CCK-8 assay. After successful lentivirus transfection, decidualization was induced. RT-qPCR was used to detect the expression of PRL, IGFBP-1 and FOXO1 in blank control group, LV-GFP induction group, LV-H19-RNAi induction group and LV-H19 induction group. Results The expression of LncRNA H19 in spontaneous abortion group was lower than that in induced abortion group (P < 0.01); LncRNA H19 reached its peak at the 7th week of decidual tissue in early pregnancy (P < 0.01); After the decidualization of THESCs, the levels of PRL, IGFBP-1, FOXO1 and LncRNA H19 were higher than those of the control group (P < 0.01).The results of CCK-8 assay showed that the proliferation of THESCs was inhibited after the interference with LncRNA H19 (P < 0.01), while the proliferation of THESCs was promoted after the overexpression of LncRNA H19 (P < 0.01). The decidualization induction was performed, after interfering with and overexpressing the LncRNA H19 of THESCs. Compared with the blank control group, the LV-GFP induction group showed the increased mRNA expression of PRL (P < 0.05), IGFBP-1 (P < 0.01) and FOXO1 (P < 0.01). Compared with LV-GFP induction group, the mRNA expression of PRL (P < 0.05) and IGFBP-1 (P < 0.01) in LV-H19 induction group was increased, while the mRNA expression of PRL (P < 0.05), IGFBP-1 (P < 0.01) and FOXO1 (P < 0.01) in LV-H19-RNAi induction group was decreased. Conclusion LncRNA H19 promotes the proliferation and decidualization of endometrial stromal cells. http://aammt.tmmu.edu.cn/html/202303092.htmlncrna h19foxo1decidualizationspontaneous abortion |
| spellingShingle | WANG Tingting HUANG Jinglin MAO Yanhua ZHANG Yingfeng SUN Congcong Role of LncRNA H19 in promoting endometrial deciduation 陆军军医大学学报 lncrna h19 foxo1 decidualization spontaneous abortion |
| title | Role of LncRNA H19 in promoting endometrial deciduation |
| title_full | Role of LncRNA H19 in promoting endometrial deciduation |
| title_fullStr | Role of LncRNA H19 in promoting endometrial deciduation |
| title_full_unstemmed | Role of LncRNA H19 in promoting endometrial deciduation |
| title_short | Role of LncRNA H19 in promoting endometrial deciduation |
| title_sort | role of lncrna h19 in promoting endometrial deciduation |
| topic | lncrna h19 foxo1 decidualization spontaneous abortion |
| url | http://aammt.tmmu.edu.cn/html/202303092.htm |
| work_keys_str_mv | AT wangtingting roleoflncrnah19inpromotingendometrialdeciduation AT huangjinglin roleoflncrnah19inpromotingendometrialdeciduation AT maoyanhua roleoflncrnah19inpromotingendometrialdeciduation AT zhangyingfeng roleoflncrnah19inpromotingendometrialdeciduation AT suncongcong roleoflncrnah19inpromotingendometrialdeciduation |