Annexin A7 Regulates Endometrial Receptivity
A limited window of receptivity is a prerequisite of reproductive success. Indispensable receptivity genes include cyclooxygenase 2 (COX2), an enzyme accomplishing formation of prostaglandin E2 (PGE2). A powerful regulator of PGE2 formation is Annexin A7 (ANXA7). The present study thus explored whet...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-08-01
|
| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fcell.2020.00770/full |
| _version_ | 1819000950882304000 |
|---|---|
| author | Md Alauddin Madhuri S. Salker Anja T. Umbach Janet Rajaxavier Toshiyuki Okumura Toshiyuki Okumura Yogesh Singh Anna Wagner Sara Y. Brucker Diethelm Wallwiener Jan J. Brosens Jan J. Brosens Florian Lang |
| author_facet | Md Alauddin Madhuri S. Salker Anja T. Umbach Janet Rajaxavier Toshiyuki Okumura Toshiyuki Okumura Yogesh Singh Anna Wagner Sara Y. Brucker Diethelm Wallwiener Jan J. Brosens Jan J. Brosens Florian Lang |
| author_sort | Md Alauddin |
| collection | DOAJ |
| description | A limited window of receptivity is a prerequisite of reproductive success. Indispensable receptivity genes include cyclooxygenase 2 (COX2), an enzyme accomplishing formation of prostaglandin E2 (PGE2). A powerful regulator of PGE2 formation is Annexin A7 (ANXA7). The present study thus explored whether ANXA7 impacts on implantation and fertility. Here we show that ANXA7 is expressed in endometrial tissue and increases upon decidual transformation of human endometrial stromal cells (HESCs) in a time-dependent manner. Silencing ANXA7 significantly decreased the expression of PRL and IGFBP1, canonical decidual marker genes, but enhances COX2 and PGE2 levels. Genetic knockout of AnxA7 in mice significantly increases the number of implantation sites and litter sizes. Further, analysis of human endometrial biopsies showed that ANXA7 transcript and protein levels are decreased during the midluteal window of implantation in women suffering from recurrent pregnancy loss (RPL) when compared to subfertile patients. Taken together, the data indicate that ANXA7 has a conserved role in regulating endometrial receptivity and implantation. |
| first_indexed | 2024-12-20T22:41:28Z |
| format | Article |
| id | doaj.art-5bffca1bc42743c2aef8f4acc024adfa |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-20T22:41:28Z |
| publishDate | 2020-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-5bffca1bc42743c2aef8f4acc024adfa2022-12-21T19:24:27ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2020-08-01810.3389/fcell.2020.00770543794Annexin A7 Regulates Endometrial ReceptivityMd Alauddin0Madhuri S. Salker1Anja T. Umbach2Janet Rajaxavier3Toshiyuki Okumura4Toshiyuki Okumura5Yogesh Singh6Anna Wagner7Sara Y. Brucker8Diethelm Wallwiener9Jan J. Brosens10Jan J. Brosens11Florian Lang12Department of Women’s Health, Eberhard Karls University of Tübingen, Tübingen, GermanyDepartment of Women’s Health, Eberhard Karls University of Tübingen, Tübingen, GermanyDepartment of Physiology, Eberhard Karls University of Tübingen, Tübingen, GermanyDepartment of Women’s Health, Eberhard Karls University of Tübingen, Tübingen, GermanyDepartment of Women’s Health, Eberhard Karls University of Tübingen, Tübingen, GermanyDepartment of Obstetrics and Gynecology, Juntendo University School of Medicine, Tokyo, JapanInstitute of Medical Genetics and Applied Genomics, Eberhard Karls University of Tübingen, Tübingen, GermanyDepartment of Women’s Health, Eberhard Karls University of Tübingen, Tübingen, GermanyDepartment of Women’s Health, Eberhard Karls University of Tübingen, Tübingen, GermanyDepartment of Women’s Health, Eberhard Karls University of Tübingen, Tübingen, GermanyDivision of Biomedical Sciences, Warwick Medical School, Coventry, United KingdomTommy’s National Centre for Miscarriage Research, University Hospitals Coventry and Warwickshire NHS Trust, Coventry, United KingdomDepartment of Physiology, Eberhard Karls University of Tübingen, Tübingen, GermanyA limited window of receptivity is a prerequisite of reproductive success. Indispensable receptivity genes include cyclooxygenase 2 (COX2), an enzyme accomplishing formation of prostaglandin E2 (PGE2). A powerful regulator of PGE2 formation is Annexin A7 (ANXA7). The present study thus explored whether ANXA7 impacts on implantation and fertility. Here we show that ANXA7 is expressed in endometrial tissue and increases upon decidual transformation of human endometrial stromal cells (HESCs) in a time-dependent manner. Silencing ANXA7 significantly decreased the expression of PRL and IGFBP1, canonical decidual marker genes, but enhances COX2 and PGE2 levels. Genetic knockout of AnxA7 in mice significantly increases the number of implantation sites and litter sizes. Further, analysis of human endometrial biopsies showed that ANXA7 transcript and protein levels are decreased during the midluteal window of implantation in women suffering from recurrent pregnancy loss (RPL) when compared to subfertile patients. Taken together, the data indicate that ANXA7 has a conserved role in regulating endometrial receptivity and implantation.https://www.frontiersin.org/article/10.3389/fcell.2020.00770/fullendometriumCOX2implantationpregnancyPGE2 |
| spellingShingle | Md Alauddin Madhuri S. Salker Anja T. Umbach Janet Rajaxavier Toshiyuki Okumura Toshiyuki Okumura Yogesh Singh Anna Wagner Sara Y. Brucker Diethelm Wallwiener Jan J. Brosens Jan J. Brosens Florian Lang Annexin A7 Regulates Endometrial Receptivity Frontiers in Cell and Developmental Biology endometrium COX2 implantation pregnancy PGE2 |
| title | Annexin A7 Regulates Endometrial Receptivity |
| title_full | Annexin A7 Regulates Endometrial Receptivity |
| title_fullStr | Annexin A7 Regulates Endometrial Receptivity |
| title_full_unstemmed | Annexin A7 Regulates Endometrial Receptivity |
| title_short | Annexin A7 Regulates Endometrial Receptivity |
| title_sort | annexin a7 regulates endometrial receptivity |
| topic | endometrium COX2 implantation pregnancy PGE2 |
| url | https://www.frontiersin.org/article/10.3389/fcell.2020.00770/full |
| work_keys_str_mv | AT mdalauddin annexina7regulatesendometrialreceptivity AT madhurissalker annexina7regulatesendometrialreceptivity AT anjatumbach annexina7regulatesendometrialreceptivity AT janetrajaxavier annexina7regulatesendometrialreceptivity AT toshiyukiokumura annexina7regulatesendometrialreceptivity AT toshiyukiokumura annexina7regulatesendometrialreceptivity AT yogeshsingh annexina7regulatesendometrialreceptivity AT annawagner annexina7regulatesendometrialreceptivity AT saraybrucker annexina7regulatesendometrialreceptivity AT diethelmwallwiener annexina7regulatesendometrialreceptivity AT janjbrosens annexina7regulatesendometrialreceptivity AT janjbrosens annexina7regulatesendometrialreceptivity AT florianlang annexina7regulatesendometrialreceptivity |